Phenolic Profile, Antioxidant and Enzyme Inhibition Properties of the Chilean Endemic Plant Ovidia pillopillo (Gay) Meissner (Thymelaeaceae)

Abstract

Ovidia pillopillo (Lloime) is an endemic species of the Valdivian Forest of Chile. Little is known on the chemistry and biological activity of this plant. In this study, the phenolic profile, antioxidant capacities and enzyme inhibition capacities (against tyrosinase and cholinesterase) of the plant were investigated for the first time. The phenolic profile of the plant was obtained by UHPLC-MS fingerprinting with high resolution, which showed the presence of several flavonoids and coumarins. The antioxidant potential was measured by FRAP and ORAC (45.56 ± 1.32; 25.33 ± 1.2 μmol Trolox equivalents/g dry plant, respectively) plus ABTS and DPPH methods (IC50 = 9.95 ± 0.05 and 6.65 ± 0.5 μg/mL, respectively). Moreover, the flavonoid and phenolic contents were determined (57.33 ± 0.82 and 38.42 ± 1.32, μg of Trolox and quercetin equivalents/100 g dry weight, respectively). The ethanolic extract showed cholinesterase (IC50 = 1.94 ± 0.07 and 2.73 ± 0.05 μg/mL, for AChE and BuChE, respectively) and tyrosinase (4.92 ± 0.05 μg/mL) enzyme inhibition activities. Based on these in vitro studies, in silico simulations were performed, which determined that the major compounds as ligands likely docked in the receptors of the enzymes. These results suggest that Ovidia pillopillo produce interesting special coumarins and flavonoids, which are potential candidates for the exploration and preparation of new medicines.

Naturally Occurring Functional Ingredient from Filamentous Thermophilic Cyanobacterium Leptolyngbya sp. KC45: Phytochemical Characterizations and Their Multiple Bioactivities

“…Table 3 shows the comparison of identified compounds with previous studies [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ,…”

Screening of Monoamine Oxidase B Inhibitors from Fragaria nubicola by Ligand Fishing and Their Neuroprotective Effects

“…N5 by semipreparative HPLC (Figures S3 and S4) for NMR analysis. Finally, by comparing the MS, 1 H NMR, and 13 C NMR data (Figures S5–S8) with those reported in the literature, − the structures of compounds 1 and 2 were identified as quercetin-3- O - β - d -glucuronide-6″-methyl ester and kaempferol-3- O - β - d -glucuronide-6″-methyl ester. It is worth noting that this is the first time to isolate these two compounds from the title plant.…”

“…It is worth noting that this is the first time to isolate these two compounds from the title plant. Both compounds have been found in food or medicinal plants including Pimenta dioica, Syzygium aromaticum, Cornus officinalis, Rubus coreanum, Oxytropis lanata, Tamarix gallica, Polygonum perfoliatum, and Ovidia pillopillo. − However, their contents are rarely reported except that 51.8–374.5 μg/g of 1 in P. perfoliatum.…”

Phytochemical Characterization and In Vitro and In Silico Biological Studies from Ferns of Genus Blechnum (Blechnaceae, Polypodiales)

“…The results obtained from molecular docking were analyzed and visualized in the Discovery Studio Visualizer software [ 30 ]. Once the molecular docking was done, the best conformation at the catalytic site was analyzed by evaluating the best hydrogen bonding interactions or π interactions, including the free ligand binding energy (kcal/mol) [ 29 , 31 ].…”