Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Abstract

<jats:p>Persicaria maculosa (Polygonaceae) (known as lady’s thumb) is an annual morphologically variable weed that is widely distributed in Chile. The purpose of this study was to investigate the antifeedant potential of methanolic (MeOH), ethanolic (EtOH), and dichloromethane (DCM) extracts from the aerial parts of this plant collected in the Valparaíso and Curicó provinces (Chile) and relate this activity to the antioxidant capacity and the presence of phenolic compounds in the extracts. A phenolic profile based on HPLC-ESI-MS/MS allowed the identification of 26 phenolic compounds, most of them glycosyl derivatives of isorhamnetin, quercetin, and kaempferol. In addition, the total phenolic content (TP), total flavonoids (TF), and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion scavenging (O2−), ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) of the extracts are reported. The antifeedant potentials of the plant extracts were tested against Epilachna paenulata, Pseudaletia adultera, Macrosiphum euphorbiae, and Diaphorina citri insects for the first time. The activity against the aphid M. euphorbiae was significant for the DCM extracts of plants from Valparaíso and Curicó (settling % = 23% ± 4% and 23% ± 5%, respectively). The antifeedant activities against the beetle E. paenulata and the lepidoptera P. adultera were significant for Valparaíso extracts, especially when tested against E. Paenulata (IFP = 1.0 ± 0.0). Finally, the MeOH and EtOH extracts from Valparaíso plants reduced the diet consumption of the psilid D. citri (p &lt; 0.05). The results showed that P. maculosa is a good source of flavonoids with some antioxidant capacities and has potential interest as botanical eco-friendly alternative with deterrent activity.</jats:p>

UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries

“…The results are summarized in Table 2 . To determine the antioxidant activity of a sample, it is important to combine several antioxidant experiments (e.g., DPPH, FRAP, and ORAC, among others) as these provide useful information on the interaction between radicals and samples, and also provide complementary information on reactive oxygen species (ROS) [ 23 , 58 ]. The results of TP, TF, TA, and the antioxidant activity were compared with previously published data related to G. phillyreifolia , G. poeppigii [ 11 ], G. shallon [ 59 ], and G. erecta [ 60 ] berries.…”

Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry

“…Supplementary Materials: The following are available online. Figure S1 (a-v): Full MS spectra and structures of compounds 1,3,7,9,12,13,15,25,27,28,29,35,37,42,43,44,46,48,51,53, and 55; Table S1: Binding energies obtained from docking experiments of most abundant compounds in G. sphacelata's fruit and the known cholinesterase inhibitor galantamine over acetylcholinesterase (TcAChE) and butyrylcholinesterase (hBuChE); Figure S2: Components of G. sphacelata fruits used in docking studies Figure S3: Predicted binding mode and predicted intermolecular interactions of all most abundant compounds in G. sphacelata pulp and seeds extracts and the residues of Torpedo californica acetylcholinesterase (TcAChE) catalytic site; Figure S4: Predicted binding mode and predicted intermolecular interactions of all most abundant compounds in G. sphacelata pulp and seeds extracts and the residues of human butyrylcholinesterase (hBuChE) catalytic site.…”

“…The total phenolic contents and total flavonoid content of the G. sphacelata fruits were measured using the Folin-Ciocalteu and FeCl 3 method previously described by our work with some modifications [35]. For TP, the results were expressed as mg of gallic acid equivalents per gram of dry fruit.…”