Phenolic extractives of wound-associated wood of beech and their fungicidal effect

Yield of Stirred Cold Maceration and Extraction of Milled European Black Alder Wood and Bark using Different Solvents

“…Total soluble flavonoid (TSF) levels in the extracts were measured using the AlCl3 spectrophotometric method (Brighente et al 2007;Diouf et al 2009;Vek et al 2013b) with some minor modifications. Prior to analysis, extracts were tempered in a water bath at 20 °C.…”

Content of Total Phenols in Red Heart and Wound-Associated Wood in Beech (Fagus sylvatica L.)

“…Hydrophilic extractives from wound-associated wood of beech (Fagus sylvatica L.) could represent attractive substances for industrial production. Our recent research showed that extracts of wound-associated wood of beech inhibited the growth of some wood decaying fungi (Vek et al, 2013) and, therefore, might be utilized as wood preservatives. It was also proven that some wood extractives have antioxidative properties (Pietarinen et al, 2006) and can potentially be used as technical antioxidants.…”

Quantitative HPLC Analysis of Catechin in Wound- Associated Wood and Knots of Beech

“…Ključne riječi: katehin, tekućinska kromatografija, ranjeno drvo, kvrge, Fagus sylvatica that fungicidal properties on wood decaying fungi can be ascribed to extracts of wound-wood, as well as to that of healthy sapwood of beech (Vek et al, 2013a). Recent chromatographic research on hydrophilic extractives of discolored and normal wood demonstrated that catechin is the dominant phenolic compound in beechwood (Baum and Schwarze, 2002 Jamalirad et al, 2011;, but there is no information on the occurrence of catechin in wound-associated beechwood.…”

“…This includes the development of discoloration and eventual decay, accompanied by the formation of reaction zones, which separate compromised and sound sapwood, and the formation of wound-wood, which attempts to overgrow the wound. These compartmentalization barriers that try to block spreading of negative consequences of wounding towards intact and vital tissues, are generally described by anatomical alterations and cell necrosis accompanied with the accumulation of antimicrobial compounds and formation of polyphenol deposits (Shortle, 1979;Bauch, 1984;Shigo, 1986 It has been recently showed by spectrophotometric analysis that the content of total phenols, flavonoids and proanthocyanidins is markedly lower in red heart than in reaction zones and wound-wood extracts (Vek et al, 2013a;2013b). It was reported that differences in the content of total phenols in reaction zones indicate differences in their formation process, differences in alterations to surrounding tissues and in the characteristics of individual trees (Vek et al, 2013b).…”

“…It appears that a high content of catechin in the tissue could have an important function in restricting the growth of wood decaying fungi. It has been recently demonstrated that methanolic extracts of wound-wood and sapwood inhibited the growth of wood decaying fungi, whereas extracts of reaction zones did not have an inhibitory effect (Vek et al, 2013a). Extract of knots were not included in our antifungal experiment.…”