Quantitative HPLC Analysis of Catechin in Wound- Associated Wood and Knots of Beech

Vek, Viljem; Oven, Primož; Poljanšek, Ida

doi:10.5552/drind.2013.1307

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Identification of extractives by HPLC: Extracts obtained by ASE were evaporated to dryness, dissolved in MeOH and filtered through a 0.22 μm polyamide filter. Chemical screening of the extractives and purity evaluation of PS and PSMME were done by HPLC-photodiode array (HPLC-PDA) analysis (Accela 600, Thermo Scientific, Waltham, MA, USA) according to Vek et al (2013) based on an octadecylsilyl (ODS) column (150 mm × 4.6 mm i.d., 2.6 μm particle size, Accucore, Thermo Scientific), in which water and MeOH served as the mobile phase (1000 μl min −1 , total analysis time of 30.2 min). The gradient system of elution: 5% → 95% solvent B in 20 min with the following steps: 0-1 min: 5% B constant; 1-21 min: 5%-95% phase B; 21-23 min: 95% phase B constant; 23-23.10 min: 95%-100% phase B; 23.10-25.10 min: 100% phase B constant; 25.10-25.20 min: 100%-5% phase B, and finally: 25.20-30.20 min 5% phase B constant before each new injection.…”

Section: Identification Of Extractives Using Gc-msmentioning

confidence: 99%

Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents

et al. 2018

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The aim of the study was to find appropriate non-chlorinated solvent systems for the extraction and separation of pure pinosylvin (PS) and pinosylvin monomethylether (PSMME) from Scots pine/Norway spruce wood residues. The extraction was performed in a pressurized solvent extractor and the purification and isolation of pure substances was done by flash chromatography. More precisely, pure PS was isolated via sequential flash chromatography in two repetitions in the gradient mode of the mobile phase, with cylcohexane (CX) and ethylacetate (EtOAc), beginning with 100% CX and ending up with 100% EtOAc. The extractives were qualitatively and quantitatively analyzed in terms of PS and PSMME contents by gas chromatography-flame ionization detector (GC-FID), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC) and proton nuclear magnetic resonance (1H-NMR) spectroscopy.

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Section: Identification Of Extractives Using Gc-msmentioning

confidence: 99%

Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents

et al. 2018

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“…It possess powerful radical scavenging activity due to the presence of a catechol moiety on ring B and hydroxyl groups on ring C [15]. Due to its antimicrobial and antifungal properties, catechin can potentially be applied as wood protection agent [16][17][18]. It is reported that the antioxidant and free radical scavenging activities of flavonoids, especially catechin, degrade wood polymers by inhibiting free radicals formed by wood rooting fungi, and participate in the natural durability of wood against fungi [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

One Step Regioselective Acylation of Polyphenolic Wood Extractive and Its Application for Wood Treatment

Sahmim¹,

Eid²,

Boer³

et al. 2022

Journal of Renewable Materials

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This study evaluated the methods of grafting commercial catechin with fatty acids, namely capric acid (C10), lauric acid (C12), and myristic acid (C14) through esterification. Specimens of beech wood (Fagus sylvatica L.) were impregnated with catechin and modified catechin-fatty acids, separately, at a 5% concentration diluted in ethanol using vacuum pressure treatment and subjected to leaching. The weight percentage gain before leaching (WPG), after leaching (WPGAL), and weight loss due to leaching (PL) were investigated. Both leached and unleached samples were tested against white-rot fungi (Trametes versicolor) in Petri-dishes for twelve weeks. Results show that samples treated with modified catechin-fatty acids provide improved resistance towards leaching. Catechin-C14 was found to be more promising, possibly due to its chain length. The decay weight loss for samples treated with modified catechin-fatty acids does not differ significantly between the samples that leached and not. Despite the antifungal properties of catechin, the treatment with catechin alone was insufficient to protect wood samples from fungi. Further, it is recommended to increase the concentration level of modified catechin to obtain a significant effect on the decay resistance.

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Quantitative HPLC Analysis of Catechin in Wound- Associated Wood and Knots of Beech

Cited by 2 publications

References 28 publications

Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents

Isolation of pure pinosylvins from industrial knotwood residue with non-chlorinated solvents

One Step Regioselective Acylation of Polyphenolic Wood Extractive and Its Application for Wood Treatment

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