Phenolic Content of Hypodaphnis Zenkeri and Its Antioxidant Effects against Fenton Reactions’ Mediated Oxidative Injuries on Liver Homogenate

Abstract

Under oxidative stress conditions, endogenous antioxidant defenses are unable to completely inactivate the free radicals generated by an excessive production of reactive oxygen species (ROS). This state causes serious cell damage leading to a variety of human diseases. Natural antioxidants can protect cells against oxidative stress. Hypaodaphnis zenkeri (H. zenkiri) is a plant consumed as a spice in the Cameroonian diet, and its bark has been used in traditional medicine for the treatment of several diseases. The present study aims at investigating the antioxidant activity, which includes free radical scavenging and protective properties of an extract from H. Zenkiri against oxidative damage on a liver homogenate. The free radical assays determined the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitrite oxide (NO) and 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals and the enzymes, whose protection was to be considered in the liver homogenate, including superoxide dismutase, catalase, and peroxidase. The antioxidative activities were studied using the ferric reducing antioxidant power (FRAP), reductive activity, and phosphomolybdenum antioxidant power (PAP) methods. In addition, the phenolic contents of the extracts were examined. The results showed that these extracts demonstrated significant scavenging properties and antioxidant activities, with the hydro-ethanolic extract of the bark of H. zenkeri (EEH) being the most potent. This extract had the highest total polyphenol (21.77 ± 0.05 mg caffeic acid (CAE)/g dried extract (DE)) and flavonoids (3.34 ± 0.13 mg quercetin (QE)/g dried extract) content. The same extract had significantly greater protective effects on enzyme activities compared to other extracts. The high performance liquied chromatography (HPLC) profile showed higher levels of caffeic acid, OH-tyrosol acid, and rutin in the leaves compared to the bark of H. zenkeri. In conclusion, the ethanolic and hydro-ethanolic extracts of the bark and leaves from H. zenkeri showed an antioxidant and protective potential against oxidative damage.

In VitroIon Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks ofTetrapleura tetrapteraand Their Protective Effects against Fenton Mediated Toxicity of Metal Ions on Liver Homogenates

“…The lipid hydroperoxides produced can undergo reductive cleavage by reduced metals such as Fe 2+ and produce lipid alkoxyl radical which can initiate additional chain reactions [33]. The final stable products of peroxidation are aldehydes which react with thiobarbituric acid (TBA) to form TBA-malonaldehyde adduct with an absorbance maximum at 532 nm [4, 13]. In the present study, T. tetraptera extracts showed a higher inhibition of the lipid peroxidation which characterized a decrease of the level of MDA.…”

“…These species are highly reactive and short-lived because they possess one or more unpaired electrons, an unpaired electron being one that is alone in an orbital. The ROS create a disequilibrium state in the cell named oxidative stress (OS) which is a cellular redox imbalance in favor of prooxidant [3, 4]. The OS in his turn causes the damage of proteins, the peroxidation of lipids, and the alteration of DNA [5].…”

“…Strong recommendations for consumption of nutraceuticals from plant origin have become progressively popular to improve health and to prevent and treat diseases [12]. Nutraceuticals are naturally derived bioactive compounds that are found in foods, dietary supplements, and herbal products and have health promoting, disease preventing, and medicinal properties [4, 13]. Food components have been for long studied for their biological activities including antioxidative and protective potential against ion-oxidative mediated toxicity [3, 14–17].…”

In vitro organo-protective effect of bark extracts from Syzygium guineense var macrocarpum against ferric-nitrilotriacetate-induced stress in wistar rats homogenates

“…The polyphenolic profile was determined according to a previously described method [28]. The analysis was performed on an Agilent Technologies 1200 HPLC system fitted with a SUPELCOSIL LC-18 column (length 250 mm, diameter 4.6 mm, packaging size 5 mm).…”

Analysis of in vitro antioxidant activity of Caryota urens L. leaves: A traditional natural remedy

“…Once an excess amount of free radicals is produced, they can reduce endogenous defensive enzymes and consequently generate deleterious cellular effects by oxidizing biomolecules like membrane lipids, cellular proteins, enzymes, DNA and RNA, later ending cellular respiration [42]. Numerous studies have revealed that antioxidants obtained from plant may be beneficial in preventing or slowing the destructive outcome of oxidative stress [43][44][45]. Herein, the antioxidant activity of CEE and its resulting fractions at several concentrations were tested.…”