Phenolic Constituents from Alchemilla vulgaris L. and Alchemilla mollis (Buser) Rothm. at Different Dates of Harvest

Abstract

Acetone/water extracts from the leaves, including stalks, of Alchemilla vulgaris L. and A. mollis (Buser) Rothm. were investigated for their phenolic composition by liquid chromatography- tandem mass spectrometry (LC-MS/MS). A total of 24 and 27 compounds were detected for A. vulgaris and A. mollis, respectively. Pedunculagin and agrimoniin, as described in earlier reports for A. vulgaris, as well as other monomeric and oligomeric ellagitannins such as sanguiin H-10, castalagin/vescalagin, and galloyl-bis-hexahydroxydiphenoyl (HHDP) hexose constituted the major phenolic fraction of both plant species. Also, gallic and chlorogenic acids were found in both extracts. Interestingly, catechin and a procyanidin trimer were detected only in A. mollis. The fl avonoid fraction comprised quercetin glucuronide as major compound in addition to several other quercetin glycosides. Most interestingly, a tentatively identifi ed kaempferol glucuronide and a methylated quercetin glucuronide were exclusively found in A. mollis. Finally, the overall phenolic fingerprints of both Alchemilla species, harvested in May and August, i.e. at the beginning and the end of the flowering period, were compared. A general accumulation of phenolic constituents was observed later in the year, especially with regard to the ellagitannins.

Phenolome of Asian Agrimony Tea (Agrimonia asiatica Juz., Rosaceae): LC-MS Profile, α-Glucosidase Inhibitory Potential and Stability

“…A similar phenomenon was observed for the genus Alchemilla . In A. vulgaris and A. mollis the content of agrimoniin ( 36 ) increased by the end of the mass flowering period by more than 100% [ 77 ]. The presence of potentillin ( 32 ) in all seasonal samples is probably because it is a main intermediate metabolite in the production of agrimoniin.…”

Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties

“…The health-benets of this phenol acid is correlated to several health benets including antioxidant, anticancer, antimutagenic, anti-diabetic effects. 29,30 Duckstein et al 31 have detected in Alchemilla vulgaris extracts a signicant concentration of phenolic acids, such as chlorogenic, gallic, ellagic acids in lower concentrations. Besides phenolic acids, the avonoids catechin, and quercetin were recorded in signicant concentration in EA extract, where catechin was the most dominant (8144.98 mg g À1 ).…”

“…A plenty of studies supported various health benets. This phenolic compound is reputable as one of the most powerful antioxidant, 31,32 while several studies have shown the a strong connection between catechin and inhibition of cancerogenesis, such like suppression on breast and ovarian cancer cell growth. 33,34 Flavonoid glucosides were identied in all tested extracts, and generally their concentration were the most abundant in EA extract.…”

Effects of <i>Alchemilla mollis</i> and <i>Alchemilla persica</i> on the wound healing process

“…By using liquid chromatography-tandem mass spectrometry technique, monomeric and oligomeric ellagitannins were identified in acetone/water extracts obtained from the leaves of A. mollis (Duckstein et al, 2012). In our previous study, hyperoside and isoquercetin quantification in aqueous methanolic extracts of A. mollis and A. persica was conducted by HPLC.…”