Phenolome of Asian Agrimony Tea (Agrimonia asiatica Juz., Rosaceae): LC-MS Profile, α-Glucosidase Inhibitory Potential and Stability

Abstract

Functional beverages constitute the rapidly increasing part of the functional food section and represent an area with a wide range of products including herbal-based beverages. We carried out screening investigations of the extracts of 85 Rosaceous tea plants. Among the extracts analyzed Agrimonia asiatica herb extract demonstrated the highest inhibitory activity against the enzyme α-glucosidase (20.29 µg/mL). As a result of chromato-mass-spectrometric profiling of A. asiatica herb with high-performance liquid chromatography with photodiode array and electrospray triple quadrupole mass-spectrometric detection (HPLC-PDA-ESI-tQ-MS) 60 compounds were identified, including catechins, ellagitannins, flavones, flavonols, gallotannins, hydroxycinnamates, procyanidins, most for the very first time. The analysis of the seasonal variation of metabolites in A. asiatica herb demonstrated that the phenolic content was highest in summer samples and lower in spring and autumn. HPLC activity-based profiling was utilized to identify compounds of A. asiatica herb with the maximal α-glucosidase inhibitory activity. The most pronounced inhibition of α-glucosidase was observed for agrimoniin, while less significant results of inhibition were revealed for ellagic acid and isoquercitrin. The evaluation of phenolic content in A. asiatica herbal teas with the subsequent determination of α-glucosidase inhibiting potential was discovered. Maximum inhibition of α-glucosidase was observed for hot infusion (75.33 µg/mL) and the minimum for 30 min decoction (159.14 µg/mL). Our study demonstrated that A. asiatica herbal tea is a prospective functional beverage in which dietary intake may help to reduce blood glucose.

Water-Soluble Melanoidin Pigment as a New Antioxidant Component of Fermented Willowherb Leaves (Epilobium angustifolium)

“…The changes of low-molecular-weight phenolics before and after the fermentation of E. angustifolium leaves were precisely analysed by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA–ESI-tQ-MS). Finally, 59 compounds were identified after comparison of their chromatographic behaviour, ultraviolet, and mass spectra with reference substances (28 compounds) and literature data [ 27 , 28 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ] ( Figure 2 , Table 2 ). Only 23 of the compounds had been found in E. angustifolium previously [ 43 , 45 , 54 ]; 36 compounds were detected for the first time.…”

Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome

“…In R. matsumuranus leaves, 21 compounds were determined to be flavonols in glycoside form. By comparing their mass spectral data with those reported previously [25][26][27][28][29] and with reference standards, these flavonols were identified as derivatives of quercetin (12 compounds) and kaempferol (9 compounds). The quercetin group of flavonols was the largest with non-acylated and acylated fragments linked with carbohydrate fragments.…”

“…Infusions and decoctions of R. matsumuranus were prepared using the optimal parameters for the extraction of ellagitannin-containing raw materials [27]. To prepare the infusions, accurately weighed leaves (1 g) were mixed with 100 mL of water (20,50,80, or 100 • C) in a conical flask and agitated (40 min), followed by cooling at 20 • C (if required), filtering through a PTFE filter (0.45 µm) into a volumetric flask (100 mL), and reducing the final volume with distilled water.…”

Antioxidant, Antidiabetic, and Antiobesity Properties, TC7-Cell Cytotoxicity and Uptake of Achyrocline satureioides (Marcela) Conventional and High Pressure-Assisted Extracts

“…In accordance with the present work, quercetin has shown to possess more α-amylase inhibition capacity than acarbose [ 32 ]. Furthermore, other extracts from medicinal plants/herbs (Vietnamese and Amazonian plants, Agrimonia asiatica , species of Myrcia genus and Euphorbia hirta herbs) possessing phenolic acids (e.g., gallic acid) and flavonoids (quercetin and/or quercetin derivatives, and luteolin) like marcela extracts, have shown antidiabetic properties (α-amylase and α-glucosidase inhibition capacity) [ 33 , 34 , 35 , 36 ]. Particularly, Euphorbia hirta L. extract has shown to lower fast blood glucose level after 4 h and a significant reduction after 15 days treatment in streptozotocin-diabetic mice [ 36 ].…”