Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts

Abstract

Crataegus (Hawthorn) has long been used as a folk medicine and is widely utilized in pharmaceutical preparations mainly because of its neuro- and cardiosedative actions and its low toxicity. The pharmacological effects of Crataegus have mainly been attributed to the polyphenolic contents. In this study, the production of polyphenols by ten-year-old Crataegus monogyna calli was studied in relation to growth variation and antioxidant capacity within a subculture period. Assays based on the Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and stability in oil-in-water emulsion were used to characterize the antioxidant actions of the callus cultures. High TEAC (3.66 micromol/g dry weight) and FRAP (208.19 micromol Fe2+/g dry weight) values were observed when maximal growth was reached(days 30-35), and this seemed to be influenced by optimum total phenol (47.40 mg/g dry weight), proanthocyanidin (20.81 mg/g dry weight), flavonoid (7.01 mg/g dry weight), anthocyanin (6.18 mg/g dry weight), (-)-epicatechin (1.77 mgl/g dry weight), procyanidin B2 (3.97 mg/g dry weight), and chlorogenic acid (1.11 mg/g dry weight) production during that period. The TEAC values were strongly associated with total flavonoids and to a lesser extent with total phenols, anthocyanins and total proanthocyanidins. The FRAP antioxidant values correlated to total phenols, proanthocyanidins and flavonoids, respectively. The polyphenolic rich calli were as effective as butylated hydroxytoluene (BHT) in preventing hydroperoxide and conjugated diene formation in a 30% oil-in-water emulsion prepared with stripped sunflower oil, during 7days storage at 30 degrees C. Crataegus monogyna cell culture represents an important alternative source for natural antioxidants.

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

“…They found that the scavenging activities of the cell suspension extracts were similar to those of the flowers. Furthermore, some reports studied the antioxidant actions of Crataegus monogyna callus cultures and showed that a high TEAC values were observed when the culture medium contained 2.0 mg/L 2,4-D and 0.5 mg/L KIN, these values were strongly influenced by the total flavonoids content (Bahorun et al 1994(Bahorun et al , 2003. In addition, Bahri-Sahloul et al showed a high DPPH radical scavenging effect in Salvia officinalis shoot cultures grown on MS medium containing a high ratio of BAP over IAA which was comparable to that of collected plants.…”

Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts

“…The same procedure was repeated with all standard gallic acid solutions (10-200 µg/ml).The amount of total polyphenols in different extracts were expressed as µg of gallic acid equivalent (GAE)/ mg extract. Flavonoids were quantified using aluminum chloride reagent (AlCl3) method described by [10] . The flavonoids content was expressed as quercetin equivalents (QE).…”

Free radical-scavenging activities of Crataegus monogyna extracts

“…The main biologically active substances detected in the medicinal vegetal raw material of monogynous hawthorn are flavonoids and their glycosides: hyperoside (quercetin-3-galactoside), quercetin, vitexin, vitexin-O-rhamnoside, isovitexin-O-rhamnoside, acetylvitexin-O-rhamnoside, rutin, quercitrin (quercetin-3-rhamnoside), orientin, kaempferol, spireoside, saponaretin, oligomeric procyanidins, catechins, and phenolic acids (chlorogenic acid, caffeic acid, triterpene saponins, etc.) (3,4).…”