Phenolic compounds of Triplaris gardneriana can protect cells against oxidative stress and restore oxidative balance

Triplaris gardneriana seeds extract exhibits in vitro anti-inflammatory properties in human neutrophils after oxidative treatment

“…Based on that, the promising activity of EETg against this oxidant species can serve as the basis for more elaborate antioxidant studies. Previous work showed that EETg has protected the MCF-7 (human breast adenocarcinoma) cell line against oxidative stress, as well as restored its redox balance after treatment with tert-butyl hydroperoxide (TBHP) solution or menadione (Almeida et al, 2017).…”

“…The moderate effect of EETg may be associated with its low permeability due to the insufficient quantity of lipophilic antioxidant compounds that could interact with the cell membrane and reach the cytosol of the tested cells (Tsumbu et al, 2012). Previous work identified the presence of quercetin as a constituent of EETg, as well as other flavonoids with intermediate lipophilic character such as catechin, quercitrin and kaempferol (Almeida et al, 2017). Thus, it is possible to infer that, perhaps, the mode of action of the extract is similar to that already described for quercetin purified or that is partly justified by the presence of quercetin in EETg composition.…”

“…Molecular docking was used to understand the binding modes of the major phenolic compounds from EETg (caffeic acid, catechin, chlorogenic acid, ellagic acid, epicatechin, gallic acid, kaempferol, quercetin, and quercitrin), previously identified and quantified by our research group (Almeida et al, 2017), in MPO protein. According to the docking, except for gallic acid, all the compounds interact in the active site of MPO (Fig.…”

“…Phytochemical investigations revealed terpenes, saponins and phenolic compounds in T. gardneriana seeds. Previous study from our research group was able to establish its phenolic profile using HPLC-DAD technique, revealing the presence of caffeic, ellagic and chlorogenic acids (phenolic acids) along with catechin, epicatechin, kaempferol, quercetin and quercitrin (flavonoids) as major phenolic entities (Almeida et al, 2017).…”

Caracterização farmacognóstica, térmica e morfológica das cascas e folhas de Triplaris gardneriana Weed

“…Recentemente, estudos por CLAE-DAD identificou ácidos fenólicos e flavonoides nas sementes (ALMEIDA et al, 2017;LOPES NETO et al, 2017). Em outros estudos, lupeol e quercetina foram isolados por cromatografia clássica e quercetina-hexosídeo, quercetina-pentosídeo, miricetinahexosídeo e quercetina-ramnobiosídeo foram identificados pela técnica hifenada LC-MS nas folhas (MACÊDO et al, 2015;MACÊDO et al, 2019), além da determinação da composição do óleo fixo das folhas e sementes por CG-MS (MACÊDO et al, 2016…”