Phenolic Compounds of Endemic Buxus Plants in Caspian Hyrcanian Forest (Buxus Hyrcana Pojark) and Their Biological Activities

Food Science & Nutrition

Oskoueian³

2022

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Background Artemisia aucheri contains antibacterial phenolic compounds. The current work was implemented to evaluate the effectiveness of a nanoliposome‐encapsulated phenolic‐rich fraction (PRF‐NLs), as a dietary phytobiotic derived from Artemisia aucheri's areal parts, on the inhibition of enteropathogenic Campylobacter jejuni (C. jejuni) infection in mice. Methods The phenolic‐rich fraction was loaded into the nanoliposome structure to obtain a nanometer‐scale size liposome with homogenous dispersion. Next, 40 white male balb/c mice were assigned to 4 treatment groups. The PRF‐NLs antibacterial potential was evaluated by evaluating the blood parameters, liver lipid peroxidation, and gene expression profiling in the mice challenged by C. jejuni infection. Results Mice infected by C. jejuni showed impairment in food intake, weight gain, liver function, ileum morphometric features, and ileum tissue inflammation. The diet of fortified food with the nonencapsulated and nanoliposome‐encapsulated phenolic compounds was found to improve these parameters at 10 mg TPC/kg BW/day concentration. Our data indicated that the nanoliposome‐encapsulated PRF was more effective in promoting the health parameters in mice as compared to nonencapsulated PRF. Conclusion It could be concluded that the liposomal encapsulation can promote the solubility, availability, and effectiveness of Artemisia aucheri phenolic compounds playing a key role as phytobiotic in mice intervened by enteropathogenic C. jejuni.

Section: Methodsmentioning

confidence: 99%

Nano‐liposomal encapsulation of Artemisia aucheri phenolics as a potential phytobiotic against Campylobacter jejuni infection in mice

Mehdizadeh

Food Science & Nutrition

Oskoueian³

2022

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“…We cleaned, separated, and freeze dried the freshly cut aerial parts of A. millefolium in the first step. Using the reflux method, 900 ml aqueous methanol (80% v/v) and 100 ml of 6 M HCl were added to 100 g of the dried powder for 2 h (Karimi et al, 2019) to do the extraction. An electronic rotary evaporator (Buchi, Flawil, Switzerland) was then used to evaporate the extract (60 °C).…”

Section: Fractionation and Total Phenolic Determinationmentioning

confidence: 99%

Nanoliposome-Encapsulated and Non-Encapsulated Phenolics From Achillea millefolium and Their Biological Function in Mice Challenged by Campylobacter jejuni: A Comparative Study

Nateghi

Oskoueian³

2022

Front. Mol. Biosci.

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The objective of this research was to develop the nanoliposome-encapsulated phenolic rich fraction from Achillea millefolium (A. millefolium) and to investigate its antibacterial and health-promoting activities in mice challenged by pathogenic foodborne Campylobacter jejuni. The A. millefolium was extracted and the ethyl acetate fraction was found to be the phenolic-rich fraction (PRF) containing 14.72 ± 2.39 mg gallic acid equivalent (GAE)/g dry weight (DM). Base on the results, the synthesized nanoliposome-loaded PRF (PRF-NLs) with the size of 187.2 nm exhibited homogeneous dispersion (PDI 0.213) and moderate stability behavior in colloidal dispersions (Zeta potential −37.45). The non-encapsulated PRF and PRF-NLs were gavaged orally in the mice for 28 days, and mice were challenged with C. jejuni on day 21. The results indicated that the dietary supplementation of non-encapsulated PRF and PRF-NLs significantly (p < 0.05) improved the average daily weight gain, food intake, liver function, antioxidant status, and morphostructural characteristics of the ileum. However, the PRF-NLs appeared to be more potent as compared to non-encapsulated PRF. The higher biological activity of PRF-NLs could be associated with the higher intestinal solubility and absorption of nanoliposome-encapsulated PRF. Thereby, the nanoliposome-encapsulated PRF could be considered as a natural antibiotic alternative called phytobiotic to prevent intestinal infection caused by enteropathogenic C. jejuni.

“…The dried leaves nely ground (powder form) using a grinder mill. Then, the 100g of the dried powder was extracted with 900 mL aqueous methanol (80% (v/v)) and 100 mL of 6 M HCl using the re ux method for 2 hours [31]. Finally, the extract was ltered and the ltrate was evaporated at the temperature of 60 o C by rotary evaporator (Buchi, Flawil, Switzerland).…”

Section: Fractionation and Total Phenolic Determinationmentioning

confidence: 99%

Nanoliposome-encapsulated Phenolic Rich Fraction From Alcea Rosea as a Dietary Phytobiotic in Mice Challenged by Escherichia Coli

Hassirian

Oskoueian

2021

Preprint

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Background: This research was performed to evaluate the antibacterial and health-promoting potentials of the nanoliposome-encapsulated phenolic rich fraction (PRF) from Alcea rosea leaves as a dietary phytobiotic in mice challenged by enteropathogenic Escherichia coli (E. coil; O157: H7). Results: The overall results demonstrated that the nanoliposome-loaded PRF contained gallic acid, salicylic acid, pyrogallol, cinnamic acid, catechin, naringin, ferulic acid. The E. coil challenge in mice impaired the weight gain, food intake, liver enzymes, lipid peroxidation, morphometric characteristics of the ileum, up-regulated the inflammatory genes (COX2, iNOS), down-regulated the antioxidant-related genes (SOD and GPx) and increased the population of E. coil in the ileum. The dietary inclusion of nonencapsulated PRF and nanoliposome-encapsulated PRF at the concentration of 10 mg TPC/kg BW/day improved these parameters however the nanoliposome-encapsulated PRF appeared to be more effective as compared to nonencapsulated PRF in improving the health parameters in mice. Conclusion: Consequently, the nanoliposome-encapsulated PRF could play a critical role as a promising phytobiotic against E. coil infection in mice.