Phenolic compounds ameliorate the glucose uptake in HepG2 cells' insulin resistance via activating AMPK

Huang, Qun; Chen, Lei; Teng, Hui; Song, Hongbo; Wu, Xiaoqi; Xu, Meiyu

doi:10.1016/j.jff.2015.09.020

Cited by 80 publications

(64 citation statements)

References 30 publications

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“…pilosa , tested in this study were preliminarily and systematically identified using RP-HPLC with UV detector by comparing with authentic compounds isolated previously (Huang et al, 2015). AM and DM were found as effective scavengers for DPPH and ABTS free radicals.…”

Section: Resultsmentioning

confidence: 99%

“…Both AM and DM were previously isolated from A. pilosa (Huang et al, 2015). The purities of AM and DM (95%) were further confirmed by using NMR and MS spectra.…”

Section: Methodsmentioning

confidence: 99%

“…We have recently isolated symbolical compounds from agrimony by utilizing antioxidant activity-guided fractionation (Huang et al, 2015). It was found that agrimonolide (AM) and desmethylagrimonilide (DM) (Figure 1) were highly presented in this Chinese edible herb, showing a powerful antioxidant activity (Huang et al, 2015) and a strong anti-diabetic potential (Chen and Kang, 2014b), and hence contributing to restore the diminished AMP-activated protein kinase (p-AMPK) levels in high-glucose-incubated adipocyte (Chen and Kang, 2014b). Therefore, the aim of the present work was to investigate compare the antioxidant activities and superoxide dismutase (SOD)-like effect in regard to underlying antioxidant and hepatoprotective mechanism of AM and DM.…”

Section: Introductionmentioning

confidence: 99%

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Agrimonolide and Desmethylagrimonolide Induced HO-1 Expression in HepG2 Cells through Nrf2-Transduction and p38 Inactivation

et al. 2017

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Agrimonolide and desmethylagrimonolide are the main bioactive polyphenols in agrimony with well-documented antioxidant, anti-diabetic, and anti-inflammatory potential. We report here for the first time that agrimonolide and desmethylagrimonolide stimulate the expression of phase II detoxifying enzymes through the Nrf2-dependent signaling pathway. Agrimonolide and desmethylagrimonolide also possess considerable protective activity from oxidative DNA damage. In order to explore the cytoprotective potential of agrimonolide and desmethylagrimonolide on oxidative stress in liver, we developed an oxidative stress model in HepG2 cells, and check the hypothesis whether Nrf2 pathway is involved. Western blotting and luciferase assay revealed that exposure of HepG2 cells to agrimonolide or desmethylagrimonolide leads to increased heme oxygenase-1 (HO-1) expression by activating ARE through induction of Nrf2 and suppression of Kelch-like ECH-associated protein 1 (Keap1). Moreover, agrimonolide and desmethylagrimonolide also activated ERK signaling pathways and significantly attenuated individual p38 MAPK expression, subsequently leading to Nrf2 nuclear translocation. In conclusion, our results indicated that transcriptional activation of Nrf2/ARE is critical in agrimonolide and desmethylagrimonolide-mediated HO-1 induction, which can be regulated partially by the blockade of p38 MAPK signaling pathway and inhibiting nuclear translocation of Nrf2.

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Section: Resultsmentioning

confidence: 99%

“…Both AM and DM were previously isolated from A. pilosa (Huang et al, 2015). The purities of AM and DM (95%) were further confirmed by using NMR and MS spectra.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Agrimonolide and Desmethylagrimonolide Induced HO-1 Expression in HepG2 Cells through Nrf2-Transduction and p38 Inactivation

et al. 2017

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“…As shown in Figure 4, (Jia, Demarco, & Sowers, 2016;Yan, Gao, Zhang, & Zhang, 2017). An earlier study by Huang et al (2015) demonstrated that phenolic compounds such as agrimonolide and desmethylagrimonolide showed the inactivation of AMPK pathways, which ameliorated the glucose uptake by HepG2 cells. Thus, the regulation of glucose uptake could be beneficial for the prevention of diabetes.…”

Section: Glucose Consumption By Hepg2 Cellsmentioning

confidence: 82%

Assessment of antioxidant and antidiabetic properties of Agaricus blazei Murill extracts

Wei

Zhan

Chen

et al. 2019

Food Science & Nutrition

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Agaricus blazei Murill (ABM), a medicinal mushroom, has beneficial effects on various human metabolic diseases. The objective of this research was to evaluate the antioxidant and antidiabetic properties of ABM extracts (ethanol extract and ethyl acetate extract). The antioxidant activities of ABM ethanol extract (EE) and ethyl acetate extract (EA) were analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radical scavenging assays and the reducing power using K 3 Fe(CN) 6 in vitro. Moreover, the effects of EE and EA on α-glucosidase inhibitory activity and improving glucose uptake by HepG2 cells were investigated in vitro. The EA showed stronger antioxidant activity, as well as inhibition of α-glucosidase, compared to EE. The analysis of glucose uptake by HepG2 cells showed that EA had significant glucose-lowering activity and exhibited no difference compared to metformin. The results suggest that ABM extracts could improve the glucose uptake by HepG2 cells and thereby alleviate postprandial hyperglycemia. This investigation provides a strong rationale for further studies on the application of ABM to control type 2 diabetes. K E Y W O R D S Agaricus blazei Murill, antidiabetic activity, antioxidant activity, HepG2 cells, α-glucosidase How to cite this article: Wei Q, Zhan Y, Chen B, et al. Assessment of antioxidant and antidiabetic properties of Agaricus blazei Murill extracts. Food Sci Nutr. 2020;8:332-339. https ://doi.

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“…Glycogen content was determined as previously described, with slight modification (Ouyang et al, 2011;Huang et al, 2015). Briefly, HepG2 cells were seeded into 6-well plates at a concentration of 2×10 5 cells/well and then treated with TBFE, TBFD, or metformin (2 mmol/L) for 24 h. Cells were collected and adjusted to the same concentration.…”

Section: Glycogen Contentmentioning

confidence: 99%

Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion

Bao

Wang²,

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography (HPLC) analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH) radical, and ferric reducing antioxidant power (FRAP)) of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species (ROS) production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in HepG2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.

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Phenolic compounds ameliorate the glucose uptake in HepG2 cells' insulin resistance via activating AMPK

Cited by 80 publications

References 30 publications

Agrimonolide and Desmethylagrimonolide Induced HO-1 Expression in HepG2 Cells through Nrf2-Transduction and p38 Inactivation

Agrimonolide and Desmethylagrimonolide Induced HO-1 Expression in HepG2 Cells through Nrf2-Transduction and p38 Inactivation

Assessment of antioxidant and antidiabetic properties of Agaricus blazei Murill extracts

Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion

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