Phenolic composition, antioxidant and anti-acetylcholinesterase activities of the TunisianScabiosa arenaria

Abstract

Context: There is a need for the discovery of novel natural antioxidants and acetylcholinesterase inhibitors (AChEIs) that are safe and effective at a global level. This is the first study on antioxidant and anti-acethylcholinesterase activity of Scabiosa arenaria Forssk (Dipsacaceae). Objective: The antioxidant potential and anti-acetylcholinesterase (AChE) activity of S. arenaria were investigated. Material and methods: The crude, ethyl acetate (EtOAc), butanol (n-BuOH) and water extracts prepared from flowers, fruits and stems and leaves of S. arenaria were tested to determine their total polyphenol content (TPC), total flavonoid content (TFC), total condensed tannin content (CTC) and their antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and b-carotene bleaching inhibition activity. Anti-AChE activity was also determined. Results: EtOAc and n-BuOH fractions of fruits had both the highest (TPC) (269.09 mg gallic acid equivalents/g dry weight). The crude extract of stems and leaves had the highest TFC (10.9 mg quercetin equivalent/g dry weight). The n-BuOH fraction of stems and leaves had the highest CTC (489.75 mg catechin equivalents/g dry weight). The EtOAc fraction of flowers exhibit a higher activity in each antioxidant system with a special attention for DPPH assay (IC 50 ¼ 0.017 mg/mL) and reducing power (EC 50 ¼ 0.02 mg/mL). The EtOAc and n-BuOH fractions of stems and leaves showed strong inhibition of AChE (IC 50 ¼ 0.016 and 0.029 mg/ mL, respectively). Discussion and conclusions: These results suggest the potential of S. arenaria as a possible source of novel compounds and as an alternative antioxidant and AChEIs.

Characterisation of phenolic antioxidants in Scabiosa arenaria flowers by LC–ESI-MS/MS and NMR

“…The combined extract was dried on a rotavapor and further fractionated with ethyl acetate and butanol. The S. arenaria ethyl acetate fraction of flowers, object of this work, was maintained at 4°C before analysis …”

“…According to these results, the ethyl acetate fraction of flowers showed a higher activity in each antioxidant system with a special attention for DPPH assay (IC50 = 0.017 ± 0.001 mg/ml) which are higher than the positive control, BHT (IC50 = 0.018 ± 0.001 mg/ml). A significant correlation was observed between the IC50 values of DPPH radical scavenging activity of the different extracts of S. arenaria flowers and the total phenolic content (TPC), r 2 = 0.711 …”

“…Previously, we have shown that Scabiosa arenaria Forssk. extracts had an interesting antioxidant activity using four assays (2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2,2‐azinobis 3‐ethyl benzo thiazoline‐6‐sulfonic acid (ABTS), reducing power and β‐carotene bleaching inhibition activity) …”

Antioxidant and anti α-glucosidase of luteolin and luteolin 7-O-glucoside isolated from Scabiosa arenaria Forssk

“…(Dipsacaceae) (S. arenaria) [14][15][16][17]. Thus, the main objective of this paper was to evaluate the antioxidant and α-glucosidase inhibitory activities of luteolin and luteolin 7-O-glucoside isolated from S. arenaria.…”

Larvicidal efficacy of Scabiosa arenaria Forssk. (Dipsacaceae) organs extracts against Culex pipiens L.

“…(S. arenaria) extracts were studied such as their antioxidant, antimicrobial, anti-glucosidase and anti-acetylcholinesterase [22][23][24][25]. However, to the best of our knowledge, no species of the family Dipsacaceae has been examined as a potential source of mosquitocides.…”