Phenolic and Flavonoid Contents of Some Plant Extracts from Tunisia Southern Landscape by Using Different Extraction Techniques: The Case of Retama reatam

Abstract

Nowadays traditional medicine, a source of several bioactive molecules for therapeutic purposes, has become a cure for various diseases. In fact, the evaluation of plant exploitation has become progressively significant and this for their therapeutic effects of many traditional medicines may be due to the immense presence of natural antioxidants. In this respect, Retama reatam was chosen among six herbal Tunisian plants traditionally known for their therapeutic virtues, as the best source of total polyphenol content (1122,927g GEA/g extract) with an important extraction yield as ethanol is the solvent. The Soxhlet extraction always has the lowest value in total polyphenol content (164,857 GEA/g extract) for such solvent. The total flavonoid content of the different extracts is not significantly different from each other. However, the yield extraction remains higher for ethanolic extraction exceeding a value of 26.7%. Concerning antioxidant activity of Retama reatam, results suggest that supercritical CO 2 extraction can be used as an efficient alternative for pre-treatment to eliminate fatty compounds and therefore evaluating oxygen radical absorbance capacity values.

Ultrasonic-assisted extraction for phenolic compounds and antioxidant activity of Moroccan Retama sphaerocarpa L. leaves: Simultaneous optimization by response surface methodology and characterization by HPLC/ESI-MS analysis

“…3 c) confirms with a compromise of 99% that setting parameters at their maximum level leads to obtaining a maximum flavonoid content equal to 45 mg QE/g DE. This TFC value remains relatively high with respect to previously reported result obtained from R. sphaerocarpa by maceration [ 43 ], and largely high compared to that recorded through maceration of R. raetam [ 42 ]. According to a similar study, choosing the appropriate ratio of ethanol to water efficiently improved the extraction of valuable compounds like flavonoids [ 49 ].…”

“…Our optimized yield was higher than that of conventional extraction methods for other retama genus. For the species Retama raetam , Rejab et al [ 42 ] have found 15.36% and 2.55% as extraction yield obtained by maceration with ethanol and hexane, respectively. Moreover, it was reported that maceration with methanol 70% gave a yield of 23.26% and 18.13% from the stems and seeds of Retama monosperma , respectively [ 43 ].…”

Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia

“…Furthermore, Maulina et al also reported that temperature and drying methods resulted in different values of total phenolic content (24). The differences in maceration methods can also affect the value of total phenolic content (25).…”

Phytochemical diversity and biological activities of Curcuma species from the East Coast of Peninsular Malaysia

“…The total flavonoid content (TFC) was determined using the aluminum chloride (AlCl3) colorimetric technique following Rejab and Ksibi (2019) with a few minor changes. About 300 L of 10% w/v sodium carbonate (NaCO3) (Sigma-Aldrich, USA) was added to an aliquot of 100 L of extract with concentrations of 20-200 g/mL in 1 mL of distilled water.…”