Ultrasonic-assisted extraction for phenolic compounds and antioxidant activity of Moroccan Retama sphaerocarpa L. leaves: Simultaneous optimization by response surface methodology and characterization by HPLC/ESI-MS analysis

Baakili, Aafaf El; Fadil, Mohamed El; Es‐Safi, Nour‐Eddine

doi:10.1016/j.heliyon.2023.e17168

Cited by 8 publications

(2 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leveraging precise mathematical and statistical techniques allows for the streamlined delineation of optimal conditions for extraction [19]. Moreover, it is extensively utilized to pinpoint the ideal conditions for operations [20–23]. In our study, we meticulously employed RSM to optimize the reflux extraction conditions for efficiently enriching target COX‐2 inhibitors from M. integrifolia .…”

Section: Introductionmentioning

confidence: 99%

Target‐guided isolation and purification of cyclooxygenase‐2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high‐speed counter‐current chromatography combined with ultrafiltration liquid chromatography

Li,

La,

Wang

et al. 2024

J of Separation Science

View full text Add to dashboard Cite

Meconopsis integrifolia (Maxim.) Franch. is used extensively in traditional Tibetan medicine for its potent anti‐inflammatory properties. In this study, six cyclooxygenase‐2 (COX‐2) inhibitors were purified from M. integrifolia using high‐speed counter‐current chromatography guided by ultrafiltration liquid chromatography (ultrafiltration‐LC). First, ultrafiltration‐LC was performed to profile the COX‐2 inhibitors in M. integrifolia. The reflux extraction conditions were further optimized using response surface methodology, and the results showed that the targeted COX‐2 inhibitors could be well enriched under the optimized extraction conditions. Then the six target COX‐2 inhibitors were separated by high‐speed countercurrent chromatography with a solvent system composed of ethyl acetate/n‐butanol/water (4:1:4, v/v/v. Finally, the six COX‐2 inhibitors, including 21.2 mg of 8‐hydroxyluteolin 7‐sophoroside, 29.6 mg of 8‐hydroxyluteolin 7‐[6′''‐acetylallosyl‐(1→2)‐glucoside], 42.5 mg of Sinocrassoside D3, 54.1 mg of Hypolaetin 7‐[6′′′‐acetylallosyll‐(l→2)‐3′′‐acetylglucoside, 30.6 mg of Hypolaetin 7‐[6′′′‐acetylallosyll‐(l→2)‐6′′‐acetylglucoside and 17.8 mg of Hypolaetin were obtained from 500 mg of sample. Their structures were elucidated by 1H‐NMR spectroscopy. This study reveals that ultrafiltration‐LC combined with high‐speed counter‐current chromatography is a robust and efficient strategy for target‐guided isolation and purification of bioactive molecules. It also enhances the scientific understanding of the anti‐inflammatory properties of M. integrifolia but also paves the way for its further medicinal applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Target‐guided isolation and purification of cyclooxygenase‐2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high‐speed counter‐current chromatography combined with ultrafiltration liquid chromatography

Li,

La,

Wang

et al. 2024

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Leveraging precise mathematical and statistical techniques, it allows for the streamlined delineation of optimal conditions for extraction [12]. Moreover, it is extensively utilized to pinpoint the ideal conditions for operation [13][14][15][16]. Consequently, we utilized RSM in this study to refine the conditions for ultrasonic-assisted extraction.…”

Section: Introductionmentioning

confidence: 99%

Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis

Li,

La,

Wang

et al. 2023

Molecules

View full text Add to dashboard Cite

Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in U. laetevirens. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/n-butanol/water (2:3:5, v/v/v) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by 1H NMR spectroscopy.

show abstract

Padina gymnospora biorefinery: coalescing ultrasonic-assisted phenolic recovery and citric acid–mediated alginate extraction

Mohanan,

Pavithra,

Kumar

et al. 2024

Biomass Conv. Bioref.

View full text Add to dashboard Cite

Ultrasonic-assisted extraction for phenolic compounds and antioxidant activity of Moroccan Retama sphaerocarpa L. leaves: Simultaneous optimization by response surface methodology and characterization by HPLC/ESI-MS analysis

Cited by 8 publications

References 76 publications

Target‐guided isolation and purification of cyclooxygenase‐2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high‐speed counter‐current chromatography combined with ultrafiltration liquid chromatography

Target‐guided isolation and purification of cyclooxygenase‐2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high‐speed counter‐current chromatography combined with ultrafiltration liquid chromatography

Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis

Padina gymnospora biorefinery: coalescing ultrasonic-assisted phenolic recovery and citric acid–mediated alginate extraction

Contact Info

Product

Resources

About