Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation

Herath, Kalahe Hewage Iresha Nadeeka Madushani; Cho, Jin-Hee; Kim, Areum; Eom, Tae Kil; Kim, Ju-Sung; Kim, Jae-Bum; Doh, Yang Hoi; Jee, Youngheun

doi:10.1016/j.jep.2018.06.008

Cited by 24 publications

(21 citation statements)

References 39 publications

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“…The preventive effects of DMLE on alcohol-induced liver injury were, therefore, measured. The hepatoprotective effect of DMLE was investigated based on a previous study wherein human liver cancer cell line HepG2 cells were used and oxidative stress was induced with ethanol [5]. The result of the MTT assay showed that cytotoxicity was absent up to the DMLE concentration of 50 µg/mL, while cell survival rate began to fall from 100 µg/mL ( Figure 4A).…”

Section: Toxicity Of Etoh and Hepatoprotective Effects Of Dmlementioning

confidence: 99%

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Dendropanax morbifera Leaf Polyphenolic Compounds: Optimal Extraction Using the Response Surface Method and Their Protective Effects against Alcohol-Induced Liver Damage

Eom

Kim

2020

Antioxidants

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The response surface methodology was used to optimally extract the antioxidant substances from Dendropanax morbifera leaves. The central composite design was used to optimally analyze the effects of ethanol concentration, sample to solvent ratio, extraction temperature, and extraction time on the total flavonoids (TF) content, ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC). All three parameters were largely influenced by the ethanol concentration and extraction temperature, while TEAC was also influenced by the sample to solvent ratio. The maximum values of TF content, FRAP, and TEAC were achieved under the following extraction conditions: 70% ethanol, 1:10 sample to solvent ratio, 80 °C, and 14 h. The D. morbifera leaf extracts (DMLE) produced under these optimum extraction conditions were investigated to determine their preventive effects on alcohol-induced liver injury. The DMLE was shown to prevent liver injury by scavenging the reactive oxygen species generated by alcohol. In addition, composition analysis of DMLE found high contents of chlorogenic acid and rutin that were determined to inhibit alcoholic liver injury. The findings of this study suggest that DMLE could prove useful as a functional food product supplement to prevent liver injury caused by excessive alcohol consumption.

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Section: Toxicity Of Etoh and Hepatoprotective Effects Of Dmlementioning

confidence: 99%

“…If the liver injury persists, the condition advances to alcoholic fatty liver disease [3,4]. A large number of recent studies have focused on developing materials from various medicinal plants that can prevent alcoholic liver injury without side effects [5,6].…”

Section: Introductionmentioning

confidence: 99%

Dendropanax morbifera Leaf Polyphenolic Compounds: Optimal Extraction Using the Response Surface Method and Their Protective Effects against Alcohol-Induced Liver Damage

Eom

Kim

2020

Antioxidants

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“…HepG2 cells have high differentiation and show many characteristics of genotypes of normal human liver hepatocytes [26]. In consequence, HepG2 cells are an ideal model system applied for studying human liver metabolism in vitro, screening the cytotoxicity capacity of novel functional molecules at the lead generation stage, and detecting environmental and dietary cytotoxins and genotoxins [27,28].…”

Section: Effects Of Peptides (M1-m10) On Hepg2 Cell Viabilitymentioning

confidence: 99%

“…Down-regulating the expression level of the gene of HMGR can lead to the rapidly reducing cholesterol biosynthesis [35]. As a nuclear receptor, PPARα highly expresses in liver and plays a crucial role in the regulation of lipid metabolism [26]. Activation of PPARα can increase the expressions levels of genes drawn into FA oxidation and transport, such as FA transport protein (FATP) and CPT-1, which lead to an increased oxidation of TG and FA and decreased synthesis of TG and FA [38].…”

Section: Effects Of Viapw (M7) and Irwww (M10) On The Expression Levementioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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“…Besides these in vivo studies, some in vitro studies also demonstrated that AR inhibitors from nature, including myricitrin [62], puerarin [63], and isoliquiritigenin [64], protected ethanol-intoxicated hepatocytes by regulating the AMPK signaling pathway. In addition, phenolic acid and flavonoid rich ethyl acetate fraction of S. quelpaertensis extract (SQEA) was reported to exert cytoprotective effect against ethanol-induced toxicity in HepG2 cells and exhibit antisteatosis effect via the activation of AMPK signaling pathway in alcohol challenged livers [65]. Interestingly, p-coumaric acid and rutin, myricetin were the predominant phenolic acid and flavonoid in SQEA, and they are all AR inhibitors.…”

Section: Role Of Plant-derived Ar Inhibitors In the Prevention Andmentioning

confidence: 99%

Aldose Reductase Inhibitors of Plant Origin in the Prevention and Treatment of Alcoholic Liver Disease: A Minireview

Qiu

Guo

Hua

2019

BioMed Research International

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Alcoholic liver disease (ALD) is caused by heavy alcohol consumption over a long period. Acetaldehyde-mediated toxicity, oxidative stress, and imbalance of lipid metabolism are generally considered involved in the initiation of ALD. There is an increasing requirement for alternative and natural medicine to treat ALD. Recently, aldose reductase (AR) has been reported to be involved in the development of ALD by affecting inflammatory cytokines, oxidative stress, and lipid metabolism. Here, we review the effect of plant-derived AR inhibitors on ALD in rodents. And we conclude that AR inhibitors of plant origin may enhance antioxidant capacity, inhibit lipid peroxidation and inflammatory cytokines expression, and activate AMP-activated protein kinase thereby subsequently suppressing alcohol-induced lipid synthesis in liver to achieve ALD protection. This review reveals that natural AR inhibitor may be potential therapeutic agent for ALD.

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Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation

Cited by 24 publications

References 39 publications

Dendropanax morbifera Leaf Polyphenolic Compounds: Optimal Extraction Using the Response Surface Method and Their Protective Effects against Alcohol-Induced Liver Damage

Dendropanax morbifera Leaf Polyphenolic Compounds: Optimal Extraction Using the Response Surface Method and Their Protective Effects against Alcohol-Induced Liver Damage

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Aldose Reductase Inhibitors of Plant Origin in the Prevention and Treatment of Alcoholic Liver Disease: A Minireview

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