Phenol biodegradation by bacterial cultures encapsulated in 3D microfiltration-membrane capsules

Kurzbaum, Eyal; Raizner, Yasmin; Kuc, Martin Esteban; Kulikov, Anatoly; Hakimi, Ben; Iasur-Kruh, Lilach; Menashe, Ofir

doi:10.1080/09593330.2019.1587005

Cited by 15 publications

(8 citation statements)

References 28 publications

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“…This finding may indicate that the capsule, as well as the 2% R2A medium ensured the survival and growth of the bacterium, which through the microfiltration membrane could gradually adapt to the presence of BTEX. Once bacterial cells had been released, the contact area between the BTEX and the biomass (the bioavailability of BTEX) increased leading to the swift and almost complete degradation of the compounds within 40 h. It is noteworthy that the SBP encapsulation technology has already been applied successfully for the treatment of industrial (olive mill wastewater) and municipal wastewater and for the biodegradation of hydrophobic and hydrophilic compounds, such as ethynylestradiol (EE2) and phenolic compounds (Azaizeh et al 2015 ; Menashe and Kurzbaum 2014 ; Kurzbaum et al 2020a , b ; Miller et al 2020 ). It has also been demonstrated that the hydrophilic cellulose acetate microfiltration membrane of the SBP capsule allows the traversing of both hydrophilic (phenols) and hydrophobic molecules (EE2).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, through the pores of the capsule, the release of CO 2 originating from the microbial degradation of a given pollutant is also possible (Menashe et al 2020b ). Additionally, the SBP technology allows the simultaneous encapsulation of easily metabolizable nutrients together with the biomass, which could facilitate the growth and increase the survival and adaptation of the encapsulated and activated biomass introduced into the contaminated environment (Kurzbaum et al 2020b ; Miller et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites

et al. 2021

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Here, we report and discuss the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites. Strain BFB1_13 was capable of degrading all the six BTEX-compounds under both aerobic (O2 conc. 8 mg l−1) and micro-aerobic/oxygen-limited (O2 conc. 0.5 mg l−1) conditions using either individual (8 mg‧l−1) or a mixture of compounds (~ 1.3 mg‧l−1 of each BTEX compound). The BTEX biodegradation capability of SBP-encapsulated cultures (SBP—Small Bioreactor Platform) was also assessed. The fastest degradation rate was observed in the case of aerobic benzene biodegradation (8 mg l−1 per 90 h). Complete biodegradation of other BTEX occurred after at least 168 h of incubation, irrespective of the oxygenation and encapsulation. No statistically significant difference was observed between aerobic and microaerobic BTEX biodegradation. Genes involved in BTEX biodegradation were annotated and degradation pathways were predicted based on whole-genome shotgun sequencing and metabolic analysis. We conclude that V. paradoxus strain BFB1_13 could be used for the development of reactive biobarriers for the containment and in situ decontamination of BTEX contaminated groundwater plumes. Our results suggest that V. paradoxus strain BFB1_13—alone or in co-culture with other BTEX degrading bacterial isolates—can be a new and efficient commercial bioremediation agent for BTEX contaminated sites.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites

et al. 2021

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“…Encapsulation is aimed at immobilizing sensitive enzymes and cells’ solutions bounded in tiny vesicles having porous membranes. Sizable enzymes cannot move out or into the capsules, yet tiny substrates/products could move freely across a semipermeable membrane . Encapsulation maintains biological systems in a fine film so as to avert the biocatalysts from contact with the environment, which might damage their efficiency.…”

Section: Immobilization Of Enzymesmentioning

confidence: 99%

Enzyme Immobilization Technologies and Industrial Applications

et al. 2023

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Enzymes play vital roles in diverse industrial sectors and are essential components of many industrial products. Immobilized enzymes possess higher resistance to environmental changes and can be recovered/recycled easily when compared to the free forms. The primary benefit of immobilization is protecting the enzymes from the harsh environmental conditions (e.g., elevated temperatures, extreme pH values, etc.). The immobilized enzymes can be utilized in various large-scale industries, e.g., medical, food, detergent, textile, and pharmaceutical industries, besides being used in water treatment plants. According to the required application, a suitable enzyme immobilization technique and suitable carrier materials are chosen. Enzyme immobilization techniques involve covalent binding, encapsulation, entrapment, adsorption, etc. This review mainly covers enzyme immobilization by various techniques and their usage in different industrial applications starting from 1992 until 2022. It also focuses on the multiscale operation of immobilized enzymes to maximize yields of certain products. Lastly, the severe consequence of the COVID-19 pandemic on global enzyme production is briefly discussed.

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“…When the SBP is coated with a semipermeable membrane, penetration of UV light into the capsule medium is prevented, thus providing a protective shield against UV irradiation. The capsules, which work well at short hydraulic retention times (HRTs) typically <6 h, [24][25][26], allow only dissolved molecules to cross the membrane while retaining the microorganisms in the capsule [27][28][29]. This protective barrier allows the microbial culture to survive and prosper under the UV irradiation treatment commonly used in AOPs.…”

Section: Introductionmentioning

confidence: 99%

UV-LED Combined with Small Bioreactor Platform (SBP) for Degradation of 17α-Ethynylestradiol (EE2) at Very Short Hydraulic Retention Time

et al. 2021

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Degradation of 17α-ethynylestradiol (EE2) and estrogenicity were examined in a novel oxidative bioreactor (OBR) that combines small bioreactor platform (SBP) capsules and UV-LED (ultraviolet light emission diode) simultaneously, using enriched water and secondary effluent. Preliminary experiments examined three UV-LED wavelengths—267, 279, and 286 nm, with (indirect photolysis) and without (direct photolysis) H2O2. The major degradation wavelength for both direct and indirect photolysis was 279 nm, while the major removal gap for direct vs. indirect degradation was at 267 nm. Reduction of EE2 was observed together with reduction of estrogenicity and mineralization, indicating that the EE2 degradation products are not estrogens. Furthermore, slight mineralization occurred with direct photolysis and more significant mineralization with the indirect process. The physical–biological OBR process showed major improvement over other processes studied here, at a very short hydraulic retention time. The OBR can feasibly replace the advanced oxidation process of UV-LED radiation with catalyst in secondary sedimentation tanks with respect to reduction ratio, and with no residual H2O2. Further research into this OBR system is warranted, not only for EE2 degradation, but also to determine its capabilities for degrading mixtures of pharmaceuticals and pesticides, both of which have a significant impact on the environment and public health.

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Phenol biodegradation by bacterial cultures encapsulated in 3D microfiltration-membrane capsules

Cited by 15 publications

References 28 publications

Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites

Potential of Variovorax paradoxus isolate BFB1_13 for bioremediation of BTEX contaminated sites

Enzyme Immobilization Technologies and Industrial Applications

UV-LED Combined with Small Bioreactor Platform (SBP) for Degradation of 17α-Ethynylestradiol (EE2) at Very Short Hydraulic Retention Time

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