Phenol and n-alkanes (C12 and C16) utilization: influence on yeast cell surface hydrophobicity

Recent biodegradation researches are focused on improving the deterioration efficiency and maintaining the stability of microbial activity during the process. Immobilizing bacterial cells by using the adhesive method is a simple way to achieve both objectives. The purpose of this study was to evaluate how the immobilization of bacterial cells by the biofilm forming method influences the biodegradation efficiency of two different bacterial consortia-low diesel oil degraders (base deterioration efficiency \ 40%) and high phenol degraders (base deterioration efficiency [ 90%). The degradation tests were carried out on four different carriers (expanded clay pellets, polypropylene, polyvinyl chloride rings and paperboard). The obtained results show that biofilms may considerably increase the efficiency of low degraders. The best diesel oil deterioration (80%) was achieved when paperboard was used as a carrier. However, the immobilization of high degraders did not significantly influence their base biodegradation potential. A 6% increase was noted only for the expanded clay pellets.

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“…Details for the GC analysis of diesel oil and phenol residues are given in the article by Chrzanowski et al (2008).…”

Section: Methodsmentioning

confidence: 99%

The influence of cell immobilization by biofilm forming on the biodegradation capabilities of bacterial consortia

Ławniczak

Kaczorek

Olszanowski

2010

World J Microbiol Biotechnol

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“…bio-Merieux, France) and molecular techniques. The yeast strains: C. maltosa EH15 and EH60 as well as Y. lipolytica EH59 and 425 were also used in experiments [10]. The mineral culture medium used throughout the studies consisted of (g/l): Na 2 ) was added.…”

Section: Microorganisms and Growth Conditionsmentioning

confidence: 99%

The Influence of Rhamnolipids on Aliphatic Fractions of Diesel Oil Biodegradation by Microorganism Combinations

et al. 2012

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Twelve different bacteria-yeast combinations were tested for determination of their ability to biodegrade diesel oil. The cell surface properties of the bacterial and yeast strains were correlated with the type of carbon source used in the experiments. The highest biodegradation of diesel oil after 7 days was obtained for the following combinations: Aeromonas hydrophila MR4-Yarrowia lipolytica EH 56 (87 %) and Xantomonas maltophila MRP7-Candida maltosa EH15 (90 %). Degradation performances of 10 of 12 combinations were enhanced by the presence of rhamnolipids. The highest increases were observed for A. hydrophila MR4-C. maltosa EH15 (from 34 to 67 %), A. hydrophila MR4-C. maltosa EH60 (from 47 to 76 %) and for Pseudomonas stutzeri MR7-C. maltosa EH60 (from 29 to 79 %). The addition of rhamnolipids to the system reduces the removal time of diesel oil from the contaminated water and changes the microbial adhesion to hydrocarbons. Modification of the cell surface of the tested strain during biodegradation is a very important factor determining the removal of hydrophobic compounds.

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“…The fact that they are nonpolar and inert organic compounds [ 6 ] represents a challenge in their use as substrates for microorganisms. The ability of microorganisms to utilize hydrocarbons as sole carbon source was first described in 1895 [ 7 ]. However, the bioavailability of hydrophobic organic compounds to microorganisms was found as a limiting step during the biodegradation process [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Using Odd-Alkanes as a Carbon Source to Increase the Content of Nutritionally Important Fatty Acids inCandida krusei, Trichosporon cutaneum,andYarrowia lipolytica

Maťátková

Gharwalová

Zimola

et al. 2017

International Journal of Analytical Chemistry

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We investigated the possibility of utilizing unusual carbon sources by three yeast strains: Candida krusei DBM 2136, Trichosporon cutaneum CCY 30-5-10, and Yarrowia lipolytica CCY 30-26-36. These strains are characterized by high biomass yield, ability to accumulate high amounts of lipids, and their potential as producers of dietetically important fatty acids. The aim of this work was the production of nutritionally important fatty acids by utilization of n-alkanes with an odd number of carbon atoms, alone and in combination with glucose and subsequent analysis of microbial lipids accumulation and fatty acid profile. All three yeast strains were able to grow and produce high amounts of the fatty acids of interest. Yarrowia lipolytica was found as the most suitable strain for the growth on n-alkanes (n-pentadecane and n-heptadecane) as the only source of carbon. The addition of biosurfactants rhamnolipids into the cultivation increased the ratio of heptadecenoic acid (up to 17.9% of total FAs in Y. lipolytica CCY 30-26-36, 14.9% in T. cutaneum CCY 30-5-10, and 17.5% in C. krusei DBM 2136) and the total biomass yield. The results show that, by manipulation of the initial cultivation conditions, the ratio of important fatty acids may be increased.

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Phenol and n-alkanes (C12 and C16) utilization: influence on yeast cell surface hydrophobicity

Cited by 30 publications

References 21 publications

The influence of cell immobilization by biofilm forming on the biodegradation capabilities of bacterial consortia

The influence of cell immobilization by biofilm forming on the biodegradation capabilities of bacterial consortia

The Influence of Rhamnolipids on Aliphatic Fractions of Diesel Oil Biodegradation by Microorganism Combinations

Using Odd-Alkanes as a Carbon Source to Increase the Content of Nutritionally Important Fatty Acids inCandida krusei, Trichosporon cutaneum,andYarrowia lipolytica

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