Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems

Clifford, Eleanor R.; Bradley, Robert W.; Wey, Laura T.; Lawrence, Joshua M.; Chen, Xiaolong; Howe, Christopher J.; Zhang, Jenny

doi:10.1039/d0sc05655c

Cited by 54 publications

(69 citation statements)

References 61 publications

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“…As an example, 2,6‐DCBQ is a quinone that prevents the cell growth at relatively low concentration. A similar effect was recently observed in the case of cyanobacteria [6b] . The results are in agreement with a redox potential‐activity relationship.…”

Section: Resultssupporting

confidence: 91%

Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions

et al. 2021

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Among all the chemical and biotechnological strategies implemented to extract energy from oxygenic photosynthesis, several concern the use of intact photosynthetic organisms (algae, cyanobacteria…). This means rerouting (fully or partially) the electron flow from the photosynthetic chain to an outer collecting electrode thus generating a photocurrent. While diverting photosynthetic electrons from living biological systems is an encouraging approach, this strategy is limited by the need to use an electron shuttle. Redox mediators that are able to interact with an embedded photosynthetic chain are rather scarce. In this respect, exogenous quinones are the most frequently used. Unfortunately, some of them also act as poisoning agents within relatively long timeframes. It thus raises the question of the best quinone. In this work, we use a previously reported electrochemical device to analyze the performance of different quinones. Photocurrents (maximum photocurrent, stability) were measured from suspensions of Chlamydomonas reinhardtii algae/quinones by chronoamperometry and compared to parameters like quinone redox potentials or cytotoxic concentration. From these results, several quinones were synthesized and analyzed in order to find the best compromise between bioelectricity production and toxicity.

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Section: Resultssupporting

confidence: 91%

Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions

et al. 2021

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“…In 2021, Zhang et al reported the first use of phenazines as diffusible redox mediators specifically in conjunction with cyanobacteria ( Synechocystis sp. PCC 6803) in biophotovoltaics [ 100 ]. Redox potentials of phenazines generally range high enough to facilitate electron transfer from the cyanobacteria and low enough to cause marginal energy losses in overpotential.…”

Section: Exogenous E-met Via Diffusible Redox Mediatorsmentioning

confidence: 99%

Rational design of artificial redox-mediating systems toward upgrading photobioelectrocatalysis

Weliwatte

Grattieri

2021

Photochem Photobiol Sci

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Photobioelectrocatalysis has recently attracted particular research interest owing to the possibility to achieve sunlight-driven biosynthesis, biosensing, power generation, and other niche applications. However, physiological incompatibilities between biohybrid components lead to poor electrical contact at the biotic-biotic and biotic-abiotic interfaces. Establishing an electrochemical communication between these different interfaces, particularly the biocatalyst-electrode interface, is critical for the performance of the photobioelectrocatalytic system. While different artificial redox mediating approaches spanning across interdisciplinary research fields have been developed in order to electrically wire biohybrid components during bioelectrocatalysis, a systematic understanding on physicochemical modulation of artificial redox mediators is further required. Herein, we review and discuss the use of diffusible redox mediators and redox polymer-based approaches in artificial redox-mediating systems, with a focus on photobioelectrocatalysis. The future possibilities of artificial redox mediator system designs are also discussed within the purview of present needs and existing research breadth.

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“…Several studies have examined the impact of both naturally occurring as well as artificially synthesized phenazine derivatives on bacterial interaction and metabolism ( Askitosari et al., 2019 ; Pierson and Pierson 2010 ; Simoska et al., 2021 ; Venkataraman et al., 2011 ). Additionally, phenazine-based redox mediators have been exogenously added to improve the overall performance of microbial electrochemical systems ( Clifford et al., 2021 ; Simoska et al., 2021 ). For example, research has demonstrated that exogenously added phenazines improve electron transfer rates in microbial fuel cells ( Rabaey et al., 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, phenazines have been used as a mediator system in microbial bioelectrochemical systems with other non-pathogen microorganisms, such as obligate aerobic Pseudomonas putida ( Askitosari et al., 2019 ; Schmitz et al., 2015 ), certain E. coli strains ( Feng et al., 2018 ), and cyanobacterium Synechocystis sp. ( Clifford et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Using structure-function relationships to understand the mechanism of phenazine-mediated extracellular electron transfer in Escherichia coli

et al. 2021

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Summary Phenazines are redox-active nitrogen-containing heterocyclic compounds that can be produced by either bacteria or synthetic approaches. As an electron shuttles (mediators), phenazines are involved in several biological processes facilitating extracellular electron transfer (EET). Therefore, it is of great importance to understand the structural and electronic properties of phenazines that promote EET in microbial electrochemical systems. Our previous study experimentally investigated a phenazine-based library as an exogenous mediator system to facilitate EET in Escherichia coli . Herein, we combine our experimental data with density functional theory (DFT) calculations and multivariate linear regression modeling to understand the structure-function relationships in phenazine-based mediated EET. These calculations demonstrate that the computed redox properties of phenazines in lipophilic environments (e.g., cell membrane) correlate to experimental mediated current densities. Additional DFT-derived molecular properties were considered to develop a predictive model, which could be used in metabolic engineering approaches to introduce phenazines as endogenous mediators into bacteria.

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Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems

Abstract: Phenazines were explored as novel low-midpoint potential molecules for wiring cyanobacteria to electrodes.

Cited by 54 publications

References 61 publications

Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions

Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions

Rational design of artificial redox-mediating systems toward upgrading photobioelectrocatalysis

Using structure-function relationships to understand the mechanism of phenazine-mediated extracellular electron transfer in Escherichia coli

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