Rational design of artificial redox-mediating systems toward upgrading photobioelectrocatalysis

Weliwatte, N. Samali; Grattieri, Matteo

doi:10.1007/s43630-021-00099-7

Cited by 33 publications

(39 citation statements)

References 205 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…32−34 Quinone-based artificial redox mediating approaches have been reported for a variety of photosynthetic organisms due to their biomimetic properties, as they compete with the natural electron carriers in the microorganisms for photoinduced electron extraction. 35 At the same time, commercial polymers or bioinspired materials have been utilized to immobilize biocatalyst, either enzymes or whole bacteria, 36 maximizing stability of the biohybrid system. 37 However, the separate synthesis of redox polymers and immobilization matrices complicate the preparation of biohybrid electrochemical systems, limiting their utilization.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Recent reports highlighted the photoinduced electron transfer process (both anodic and cathodic) in purple bacteria, , and the approaches developed to accomplish and artificially tune the extracellular electron transfer (EET) with intact photosynthetic bacteria . These include utilizing artificial redox mediating systems based on Osmium, − or quinone-based redox polymers, electrode surface engineering, and synthetic biology. − Quinone-based artificial redox mediating approaches have been reported for a variety of photosynthetic organisms due to their biomimetic properties, as they compete with the natural electron carriers in the microorganisms for photoinduced electron extraction . At the same time, commercial polymers or bioinspired materials have been utilized to immobilize biocatalyst, either enzymes or whole bacteria, maximizing stability of the biohybrid system .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Interfacing intact and metabolically active photosynthetic bacteria with abiotic electrodes requires both establishing extracellular electron transfer and immobilizing the biocatalyst on electrode surfaces. Artificial approaches for photoinduced electron harvesting through redox polymers reported in literature require the separate synthesis of artificial polymeric matrices and their subsequent combination with bacterial cells, making the development of biophotoanodes complex and less sustainable. Herein, we report a one-pot biocompatible and sustainable approach, inspired by the byssus of mussels, that provides bacterial cells adhesion on multiple surfaces under wet conditions to obtain biohybrid photoanodes with facilitated photoinduced electron harvesting. Purple bacteria were utilized as a model organism, as they are of great interest for the development of photobioelectrochemical systems for H 2 and NH 3 synthesis, biosensing, and bioremediation purposes. The polydopamine matrix preparation strategy allowed the entrapment of active purple bacteria cells by initial oxygenic polymerization followed by electrochemical polymerization. Our results unveil that the deposition of bacterial cells with simultaneous polymerization of polydopamine on the electrode surface enables a 5-fold enhancement in extracellular electron transfer at the biotic/abiotic interface while maintaining the viability of the cells. The presented approach paves the way for a more sustainable development of biohybrid photoelectrodes.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…oneidensis . With the aim to provide both artificial redox mediation and adhesive properties to maintain the bacteria on the electrode surface, redox-adhesive polymeric matrices based on polydopamine have been recently developed for E-MET with various bacteria. − As photosynthetic bacteria have intrinsic limitations to the EET due to the location of the photosynthetic apparatus inside the inner membrane, the reader is referred to a recent review discussing this aspect and presenting the various artificial redox-mediating systems specifically developed for this class of bacteria …”

Section: Principles Of Bioelectrochemistrymentioning

confidence: 99%

“…79−81 As photosynthetic bacteria have intrinsic limitations to the EET due to the location of the photosynthetic apparatus inside the inner membrane, the reader is referred to a recent review discussing this aspect and presenting the various artificial redox-mediating systems specifically developed for this class of bacteria. 82 2.4. Synthetic Biology for Electrochemistry 2.4.1.…”

Section: Extracellular Direct and Mediated Electron Transfermentioning

confidence: 99%

Enzymatic and Microbial Electrochemistry: Approaches and Methods

et al. 2022

Self Cite

View full text Add to dashboard Cite

The coupling of enzymes and/or intact bacteria with electrodes has been vastly investigated due to the wide range of existing applications. These span from biomedical and biosensing to energy production purposes and bioelectrosynthesis, whether for theoretical research or pure applied industrial processes. Both enzymes and bacteria offer a potential biotechnological alternative to noble/rare metal-dependent catalytic processes. However, when developing these biohybrid electrochemical systems, it is of the utmost importance to investigate how the approaches utilized to couple biocatalysts and electrodes influence the resulting bioelectrocatalytic response. Accordingly, this tutorial review starts by recalling some basic principles and applications of bioelectrochemistry, presenting the electrode and/or biocatalyst modifications that facilitate the interaction between the biotic and abiotic components of bioelectrochemical systems. Focus is then directed toward the methods used to evaluate the effectiveness of enzyme/bacteria−electrode interaction and the insights that they provide. The basic concepts of electrochemical methods widely employed in enzymatic and microbial electrochemistry, such as amperometry and voltammetry, are initially presented to later focus on various complementary methods such as spectroelectrochemistry, fluorescence spectroscopy and microscopy, and surface analytical/characterization techniques such as quartz crystal microbalance and atomic force microscopy. The tutorial review is thus aimed at students and graduate students approaching the field of enzymatic and microbial electrochemistry, while also providing a critical and up-to-date reference for senior researchers working in the field.

show abstract

“…Upon illumination, these photosynthetic components convert absorbed light energy into electrical current collected by the anode. Similar to some MET type MFCs, the electron transfer from these photosynthetic components to the anode may be enabled or enhanced by the addition of an exogenous mediators such as potassium ferricyanide or quinones or via tightly bound redox polymers or nanoparticles (Weliwatte et al, 2021). Although isolated photosystems can theoretically provide a high concentration of photochemical reaction centers (and thus high current densities), their isolation is more complicated and expensive than the use of isolated photosynthetic membranes or intact organisms and therefore may be less attractive for practical applications.…”

Section: Introductionmentioning

confidence: 99%

Production of photocurrent and hydrogen gas from intact plant leaves

Shlosberg¹,

Meirovitch²,

Yehezkeli³

et al. 2021

Preprint

View full text Add to dashboard Cite

Efforts to replace fossil fuels with renewable energy technologies, especially solar energy conversion, continue to improve the potential to produce useful amounts of energy without significant pollution. Utilization of photosynthetic organisms in bio-photo electrochemical cells (BPECs) are a potentially important source of clean energy. Here, we show that it is possible to harvest photocurrent directly from unprocessed plant tissues in specialized BPECs. The source of electrons are shown to originate from the Photosystem II water-oxidation reaction that results in oxygen evolution. In addition to terrestrial and crop plants, we further demonstrate the ability of the desert plant Corpuscularia lehmannii to produce bias-free photocurrent without the addition of an external electrolyte. Finally, we show the use of pond-grown water lilies to generate photocurrent. Different leaves produce photocurrent densities in the range of 1 to 10 mA / cm2 which is significantly higher than microorganism-based BPECs. The relatively high photocurrent and the simplicity of the plants BPEC may pave the way toward the establishment of first applicative photosynthetic based energy technologies.

show abstract

Rational design of artificial redox-mediating systems toward upgrading photobioelectrocatalysis

Cited by 33 publications

References 205 publications

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Enzymatic and Microbial Electrochemistry: Approaches and Methods

Production of photocurrent and hydrogen gas from intact plant leaves

Contact Info

Product

Resources

About