PHBV/bioglass composite scaffolds with co-cultures of endothelial cells and bone marrow stromal cells improve vascularization and osteogenesis for bone tissue engineering

Abstract

PHBV + 10% BG composite scaffolds stimulated osteogenic differentiation and angiogenic differentiation of co-cultures of HBMSCs and HUVECs by enhancing paracrine effects between the two types of cells.

Enhanced biomineralization and protein adsorption capacity of 3D chitosan/hydroxyapatite biomimetic scaffolds applied for bone-tissue engineering

“…The porosity of the CS and CS/HAp scaffolds was determined by the liquid substitution method, which was described previously. 11 DI water was used as the displacement liquid because it can easily move into scaffold pores without a dissolution reaction. Briey, a dry sample of each scaffold was weighed (W 1 ), aer that it was immersed in a known volume (V 1 ) of DI water inside a cylinder for 1 h to pervade water into the pores of the scaffold.…”

“…[1][2][3] At present, numerous materials have been used for the preparation of scaffolds for bone tissue regeneration; three main types of materials have been classied, including polymers, 4,5 bioceramics, [6][7][8] and composites. [9][10][11][12] Natural bone exhibits a hierarchical structure consisting of collagen brils, and hydroxyapatite nanocrystallites (nHAp), which are the organic and inorganic components, respectively. 13 For the past years, the development of articial scaffolds that mimic the native bone in terms of the complex structure has attracted scientists in the eld of BTE.…”

Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering

“…Through the formation of surface hydroxy carbonate apatite (HCA) layer, these ceramic bonds to both hard and soft tissues (Lizzi et al, 2017;Salinas & Vallet-Regí, 2013;Siqueira et al, 2017). Taking into account the previous art, bioactive glass ceramic affects the cell adhesion, proliferation, differentiation and colonization on the surface of implants (Kargozar et al, 2017;Shamsi et al, 2017;Wu, Wu, Xue, Li, & Liu, 2017;Wu, Zhou, Chang, & Xiao, 2013). Various composite hydrogels based on the bioactive glass have been synthesized recently, using biodegradable natural polymers including:…”

Hydroxyapatite based biocomposite scaffold: A highly biocompatible material for bone regeneration

“…Also, the cells were recolored utilizing the Live/dead cell measure packs for four hours. The recolored tests were washed twice with phosphate buffer solution and were seen under a fluorescent magnifying instrument ( Wu et al, 2017 ).…”