Hydroxyapatite based biocomposite scaffold: A highly biocompatible material for bone regeneration

Li, Ceng; Qin, Weiguang; Lakshmanan, Sivalingam; Ma, Xiaohui; Sun, Osbert Jianxin; Xu, Bo

doi:10.1016/j.sjbs.2020.05.029

Cited by 16 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…What is more, the corrosion resistance and biological activity of magnesium alloys and magnesium alloy composite materials can be improved as well. 24,37 . Mg-Sr alloys have been recently evaluated as potential materials for biodegradable implants.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo study on the osseointegration of magnesium and strontium ion with two different proportions of mineralized collagen and its mechanism

et al. 2021

View full text Add to dashboard Cite

To explore the optimal combination of Mg2+, Sr2+ and mineralized collagen (nHAC) with two different proportions of hydroxyapatite (HA) and collagen (COL) on differentiation of MC3T3-E1 and the underlying mechanism, as well as achieve bone osseointegration. MC3T3-E1 cells were cultured in a complete medium with Mg2+ at the concentration of 0, 4, 8, 12, 16, 20 mmol/L, Sr2+ at the concentration of 0, 3, 6, 12 mmol/L, and the impregnation solution of 3:7 and 5:5nHAC. The differentiation of MC3T3-E1 was measured by expression of osteogenic genes and proteins including Runx-2, BMP-2 and OCN and determined the activation of PI3K/AKT/GSK3β/β-catenin signaling pathway in 12 mmol/LMg 2 ++3 mmol/LSr 2 ++3:7nHAC group. Osteoporosis was induced in 18 female rats by means of ovariectomy, the implants were immersed in 60 mmol/LMg 2 ++15 mmol/LSr 2 ++3:7nHAC impregnation solution and implanted into the mesial alveolar fossa for immediate implantation. The osseointegration of the implants was observed by Confocal laser scanning microscopy (CLSM) and histology at 4 and 8 weeks. The groups cultured with 12 mmol/LMg 2 +, 3 mmol/LSr 2 + and 3:7nHAC impregnation solution showed the osteogenic genes and proteins were significantly higher respectively (P < 0.05), as well as p-Akt, p-GSK3β and β-catenin proteins (P < 0.05). CLSM and histology showed that the implant surface was surrounded by thick lamellar bone plate, and the trabecular bone were dense and continuous in the impregnation solution. These results found that magnesium and strontium ion-loaded mineralized collagen play an critical role in up-regulating the cells activity through PI3K/AKT/GSK3β/β-catenin signaling pathway and could be promote the formation of osseointegration.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo study on the osseointegration of magnesium and strontium ion with two different proportions of mineralized collagen and its mechanism

et al. 2021

View full text Add to dashboard Cite

show abstract

“…MHAP had great application potential as an engineering material for bone tissue repair, but its osteo-inductivity and osseointegration needed to be improved, so as to better meet the clinical needs. [30][31][32] Considering that MHAP had the ability to load drugs, we chose to load UA into it. The UA drug was extracted from many plants such as the whole grass of the Labiatae plant, Prunella vulgaris, and the leaves of the holly, holly, iron holly leaves.…”

Section: Morphologies and Structures Of Mhap Nanoparticlesmentioning

confidence: 99%

Ursolic Acid Loaded-Mesoporous Hydroxylapatite/ Chitosan Therapeutic Scaffolds Regulate Bone Regeneration Ability by Promoting the M2-Type Polarization of Macrophages

Yu¹,

Wang²,

Liu³

et al. 2021

IJN

View full text Add to dashboard Cite

Purpose Mesoporous hydroxylapatite (MHAP) might be important for bone regeneration, and ursolic acid (UA) has anti-inflammatory effects. Accordingly, we developed, for the first time, ursolic acid-loaded MHAP-chitosan (MHAP-CS-UA) scaffolds to treat bone defects. Methods In vitro, we synthesize biomaterial scaffolds. By SEM, XRD, EDS and FTIR, we test the performance of the hybrid scaffolds. By drug release, flow cytometry, immunofluorescence, alizarin red staining, and Western blotting, we test the anti-inflammatory and osteo-inductive properties of scaffolds. In vivo, we verify osseointegration ability and bone regeneration. Results The MHAP is a rod-shaped structure with a length of 100~300nm and a diameter of 40~60nm. The critical structure gives the micro-scaffold a property of control release due to the pore sizes of 1.6~4.3 nm in hydroxyapatite and the hydrogen bonding between the scaffolds and UA drugs. The released UA drugs could notably inhibit the polarization of macrophages to pro-inflammatory macrophages (M1 type) and promote the expression of osteogenic-related genes (COL1, ALP and OPG) and osteogenic-related proteins (BMP-2, RUNX2 and COL1). Conclusion The MHAP-CS-UA scaffolds have good anti-inflammatory, osseointegration, osteo-inductivity and bone regeneration. And they will be the novel and promising candidates to cure the bone disease.

show abstract

“…The mesoporous structure of MHAP nanoparticles provided good basic conditions for material degradation and drug loading. MHAP had great application potential as an engineering material for bone tissue repair, but its osteo-inductivity needed to be improved, so as to better meet the clinical needs [30][31][32]. Considering that MHAP had the ability to load drugs, we chose to load UA into it.…”

Section: Morphologies and Structures Of Mhap Nanoparticlesmentioning

confidence: 99%

Ursolic acid loaded-mesoporous hydroxylapatite/ chitosan therapeutic scaffolds promote the bone regeneration

Yu¹,

Wang²,

Liu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Mesoporous hydroxylapatite (MHAP) could play an important role in bone regeneration, and UA (Ursolic acid) also promote the osteogenic differentiation. Accordingly, we developed the UA loaded MHAP scaffolds to cure bone defects. In vitro, we synthesize biomaterial scaffolds. By SEM, XRD, EDS and FTIR, we test the performance of the hybrid scaffolds. By drug release, ALP staining, Alizarin red staining, and Western blotting, we test the osteo-inductive properties of scaffold materials. In vivo, We verify bone regeneration through a rat skull defect model.Results: The MHAP is a rod-shaped structure with a length of 100~300nm and a diameter of 40~60nm. The critical structure gives the micro scaffold a property of control release due to the pore sizes of 1.6~4.3 nm in hydroxyapatite and the hydrogen bonding between the scaffolds and UA drugs. The released UA drugs could notably promote the expression of osteogenic-related genes (COL1, ALP, OPG) and osteogenic-related proteins (BMP-2, RUNX2 and COL1). Both the images of μCT and the results of double fluorochrome labelling demonstrated that therapeutic scaffolds promoted the bone regeneration. We obtained the similar results through immunohistochemistry. Conclusions: The MHAP-CS-UA scaffolds have good osteo-inductivity and bone regeneration. And they will be the novel and promising candidates to cure the bone disease.

show abstract

Hydroxyapatite based biocomposite scaffold: A highly biocompatible material for bone regeneration

Cited by 16 publications

References 40 publications

In vitro and in vivo study on the osseointegration of magnesium and strontium ion with two different proportions of mineralized collagen and its mechanism

In vitro and in vivo study on the osseointegration of magnesium and strontium ion with two different proportions of mineralized collagen and its mechanism

Ursolic Acid Loaded-Mesoporous Hydroxylapatite/ Chitosan Therapeutic Scaffolds Regulate Bone Regeneration Ability by Promoting the M2-Type Polarization of Macrophages

Ursolic acid loaded-mesoporous hydroxylapatite/ chitosan therapeutic scaffolds promote the bone regeneration

Contact Info

Product

Resources

About