Phase Transition Detection in Accumulation of a Potential Anticancer Drug Cl-IPBD with DNA: Supercoiled and Linear pUC19 Plasmids

Janiszek, Dominika; Karpińska, Monika; Niewiadomy, Andrzej; Girstun, Agnieszka; Elżanowska, H.; Maj-Żurawska, Magdalena; Kulesza, Paweł J.

doi:10.1016/j.electacta.2016.05.159

Cited by 5 publications

(1 citation statement)

References 58 publications

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“…Several reviews about chemo/biosensors based on graphene can be found in the literature . One of the major aims of biosensor technology is to determine specific DNA sequences for different applications such as drugs synthesis, distinction of diseases, food technologies, and forensic science . However, the high stability of graphene surface makes the immobilization of DNA strands challenging.…”

Section: Introductionmentioning

confidence: 99%

Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor

et al. 2018

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We report the effect of electrochemical anodization on the properties of monolayer graphene as the main aim of this research and consequently using the resulting label‐free impedimetric biosensor for DNA sequences detection. Monolayer graphene was grown by chemical vapor deposition (CVD) with methane as precursor on copper foil, transferred onto a glassy carbon electrode and electrochemically anodized. Raman spectroscopy and X‐Ray photo electron spectroscopy revealed enhancement of defect density, roughness and formation of C−O−C, C−O−H and C=O functional groups after anodization. Amine‐terminated poly T probe was linked covalently to the carboxylic groups of anodized graphene by the zero‐length linker to fabricate the impedance‐based DNA biosensor. The anodized graphene electrode demonstrated a superior performance for electrochemical impedance detection of DNA. The DNA biosensor showed a large linear dynamic range from 2.0×10−18 to 1.0×10−12 M with a limit of detection of 1.0×10−18 M using electrochemical impedance spectroscopy (EIS) method. Equivalent circuit modeling shows that DNA hybridization is detected through a change in charge transfer resistance.

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Section: Introductionmentioning

confidence: 99%

Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor

et al. 2018

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Differences in electrochemical response of prospective anticancer drugs IPBD and Cl-IPBD, doxorubicin and Vitamin C at plasmid modified glassy carbon

Janiszek

Karpińska

Niewiadomy

et al. 2021

Bioelectrochemistry

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A novel quantitative electrochemical method to monitor DNA double-strand breaks caused by a DNA cleavage agent at a DNA sensor

Banasiak¹,

Cassidy²,

Colleran³

2018

Biosensors and Bioelectronics

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To date, DNA cleavage, caused by cleavage agents, has been monitored mainly by gel and capillary electrophoresis. However, these techniques are time-consuming, non-quantitative and require gel stains. In this work, a novel, simple and, importantly, a quantitative method for monitoring the DNA nuclease activity of potential anti-cancer drugs, at a DNA electrochemical sensor, is presented. The DNA sensors were prepared using thiol-modified oligonucleotides that self-assembled to create DNA monolayers at gold electrode surfaces. The quantification of DNA double-strand breaks is based on calculating the DNA surface coverage, before and after exposure to DNA cleavage agents, using a method developed by a Tarlov group. The nuclease properties of a model DNA cleavage agent, copper bisphenanthroline ([Cu II (phen) 2 ] 2+), that cleaves DNA in a Fenton-type reaction, were quantified electrochemically. The DNA surface coverage decreased on average by 21 % after immersing the DNA sensor in a nuclease assay containing [Cu II (phen) 2 ] 2+ , a reductant and an oxidant. This percentage indicates that 6 base pairs were cleaved in the nuclease assay from the immobilised 30 base pair strands. The DNA cleavage can be also induced electrochemically in the absence of a chemical reductant. [Cu II (phen) 2 ] 2+ , intercalates between DNA base pairs and, on application of a suitable potential, can be reduced to [Cu I (phen) 2 ] + , with solution oxygen acting as the required oxidant. This reduction process is facilitated through DNA strands via long-range electron transfer, resulting in DNA cleavage of 23 %. The control measurements for both chemically and electrochemically induced cleavage revealed that DNA strand breaks did not occur under experimental conditions in the absence of [Cu II (phen) 2 ] 2+ .

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Phase Transition Detection in Accumulation of a Potential Anticancer Drug Cl-IPBD with DNA: Supercoiled and Linear pUC19 Plasmids

Cited by 5 publications

References 58 publications

Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor

Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor

Differences in electrochemical response of prospective anticancer drugs IPBD and Cl-IPBD, doxorubicin and Vitamin C at plasmid modified glassy carbon

A novel quantitative electrochemical method to monitor DNA double-strand breaks caused by a DNA cleavage agent at a DNA sensor

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