Phase transferring luminescent gold nanoclusters via single-stranded DNA

Liu, Yu; Lü, Hui; Qu, Zhi; Li, Mingqiang; Zheng, Haoran; Gu, Peilin; Shi, Jiye; Li, Jiang; Li, Qian; Wang, Lihua; Chen, Jing; Fan, Chunhai

doi:10.1007/s11426-022-1238-2

Cited by 10 publications

(17 citation statements)

References 57 publications

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“…For nucleic acid chemistry, an increasing number of moieties and functional molecules have been developed, allowing for easy assembly and integration of diverse functions . The versatile intelligent DMMs were developed for a variety of biological applications, including biomolecular rulers, biosynthesis, biocomputing, and biosensing. , …”

Section: Discussionmentioning

confidence: 99%

“…77 The versatile intelligent DMMs were developed for a variety of biological applications, including biomolecular rulers, biosynthesis, biocomputing, and biosensing. 202,203 One of the most significant challenges for artificial DMMs is to approach the complexity and intricacy of natural proteins. Since current methods focus on substituting allosteric and mechano-gating coupling into DNA nanostructures, engineering mechanochemical coupling requires a diverse set of fully predictable monomeric components.…”

Section: Discussionmentioning

confidence: 99%

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DNA-Based Molecular Machines

Mao

Liu

et al. 2022

JACS Au

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Artificial molecular machines have found widespread applications ranging from fundamental studies to biomedicine. More recent advances in exploiting unique physical and chemical properties of DNA have led to the development of DNA-based artificial molecular machines. The unprecedented programmability of DNA provides a powerful means to design complex and sophisticated DNA-based molecular machines that can exert mechanical force or motion to realize complex tasks in a controllable, modular fashion. This Perspective highlights the potential and strategies to construct artificial molecular machines using double-stranded DNA, functional nucleic acids, and DNA frameworks, which enable improved control over reaction pathways and motion behaviors. We also outline the challenges and opportunities of using DNA-based molecular machines for biophysics, biosensing, and biocomputing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

DNA-Based Molecular Machines

Mao

Liu

et al. 2022

JACS Au

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“…Inspired by the structure of mechanosensitive PIEZO, we aim to devise a biomimetic mechanosensitive fluorescent probe to monitor membrane tension in lipid vesicles. We reason that self‐assembled DNA nanostructures hold great potential to mimic the structures and functions of PIEZO channels, especially given that they have been successfully utilized to design versatile nanomachines with predictable nanomechanical properties [15–19] . With appropriate computation design, DNA molecules can spontaneously fold into prescribed DNA nanostructures that have the potential to expand the toolkit of synthetic biology [20–24] .…”

Section: Introductionmentioning

confidence: 99%

“…We reason that self-assembled DNA nanostructures hold great potential to mimic the structures and functions of PIEZO channels, especially given that they have been successfully utilized to design versatile nanomachines with predictable nanomechanical properties. [15][16][17][18][19] With appropriate computation design, DNA molecules can spontaneously fold into prescribed DNA nanostructures that have the potential to expand the toolkit of synthetic biology. [20][21][22][23][24] For example, by attaching hydrophobic membrane anchors such as cholesterol (Chol) on the DNA scaffolds, a series of membrane-targeted DNA nanopores with customized parameters including diameter, height, wall thickness, and location of binding sites were designed to mimic the functions of natural protein channels.…”

Section: Introductionmentioning

confidence: 99%

A Membrane Tension‐Responsive Mechanosensitive DNA Nanomachine

Zheng

et al. 2023

Angew Chem Int Ed

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Membrane curvature reflects physical forces operating on the lipid membrane, which plays important roles in cellular processes. Here, we design a mechanosensitive DNA (MSD) nanomachine that mimics natural mechanosensitive PIEZO channels to convert the membrane tension changes of lipid vesicles with different sizes into fluorescence signals in real time. The MSD nanomachine consists of an archetypical six‐helix‐bundle DNA nanopore, cholesterol‐based membrane anchors, and a solvatochromic fluorophore, spiropyran (SP). We find that the DNA nanopore effectively amplifies subtle variations of the membrane tension, which effectively induces the isomerization of weakly emissive SP into highly emissive merocyanine isomers for visualizing membrane tension changes. By measuring the membrane tension via the fluorescence of MSD nanomachine, we establish the correlation between the membrane tension and the curvature that follows the Young‐Laplace equation. This DNA nanotechnology‐enabled strategy opens new routes to studying membrane mechanics in physiological and pathological settings.

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“…At a low ionic strength, the electrostatic repulsion between negatively charged amphiphilic nucleic acids is not sufficiently screened to promote the formation of micelles and most ssDNAs formed am-TDF via the Watson-Crick base pairing. However, under a high ionic strength, the electrostatic repulsion between nucleic acids is greatly screened, 25 and the hydrophobic forces drive the aggregation of amphiphilic ssDNAs to form micelles. Based on these results, the increased Mg 2+ could improve the energetic barrier between micelles and am-TDF (Fig.…”

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confidence: 99%