Phase Separation as a Molecular Thermosensor

Li, Changxuan; Fang, Xiuqi

doi:10.1016/j.devcel.2020.09.019

Cited by 4 publications

(1 citation statement)

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“…In metazoans, phase separation processes are used as temperature sensors in various contexts (Li and Fang, 2020). Plants, as sessile organisms, need mechanisms to acclimate to changing temperatures.…”

Section: Cp29a Droplets As a Compartment For Cold Acclimationmentioning

confidence: 99%

The prion-like domain of the chloroplast RNA binding protein CP29A is required for cold-induced phase separation next to nucleoids and supports RNA splicing and translation during cold acclimation

Legen,

Lenzen,

Kachariya

et al. 2023

Preprint

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Arabidopsis thalianais capable of producing photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif (RRM) domains of CP29A. The resulting droplets display liquid-like properties and are found in close proximity to chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role for plant cold resistance.

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Section: Cp29a Droplets As a Compartment For Cold Acclimationmentioning

confidence: 99%