Phase-sensitive lock-in detection for high-contrast mid-infrared photothermal imaging with sub-diffraction limited resolution

Samolis, Panagis; Sander, Michelle Y.

doi:10.1364/oe.27.002643

Cited by 48 publications

(40 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work, we additionally harness the capability of QPI to computationally synthesize the 3D spatial-frequency aperture of the imaging system 2,22 . This enables us to further achieve (3) the higher lateral spatial resolution by the expanded bandwidth of the synthetic aperture that surpasses the numerical aperture (NA) of a single objective lens which poses the diffraction limit in other farfield MVI techniques [6][7][8][9][10][11][12][13][14][15][16][17][18]20 and (4) decoupling the undesired MIR absorption effect of the surrounding aqueous medium, which is the well-known problem of MIR microscopy in general, by the depth-resolving power offered by the axial bandwidth of the 3D synthetic aperture.…”

Section: Mv-qpi the Concept Of Mv-qpi Is Illustrated Inmentioning

confidence: 99%

Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Tamamitsu¹,

Toda²,

Shimada³

et al. 2020

Optica

View full text Add to dashboard Cite

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morphology and biochemistry, respectively, that have pioneered numerous applications along their independent technological maturity over the past few decades. However, the distinct label-free contrasts are inherently complementary and difficult to integrate due to the use of different light-matter interactions. Here, we present a unified imaging scheme that realizes simultaneous and in-situ acquisition of MV-fingerprint contrasts of single cells in the framework of QPI utilizing the mid-infrared photothermal effect. The fully label-free and robust integration of subcellular morphology and biochemistry would have important implications, especially for studying complex and fragile biological phenomena such as drug delivery, cellular diseases and stem cell development, where long-time observation of unperturbed cells are needed under low phototoxicity.

show abstract

Section: Mv-qpi the Concept Of Mv-qpi Is Illustrated Inmentioning

confidence: 99%

Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Tamamitsu¹,

Toda²,

Shimada³

et al. 2020

Optica

View full text Add to dashboard Cite

show abstract

“…The active research activities of IR spectroscopic imaging in areas like life scicence, polymers, perovskites, and plasmonic metasurface have paved the way for spatial optical property mapping of 2D materials . The development and applications of AFM‐IR have been reviewed in the fields of polymers, cells and tissues, and energy materials .…”

Section: Spectroscopic Imagingmentioning

confidence: 99%

Microscale Spectroscopic Mapping of 2D Optical Materials

Dong

Yetisen

Köhler

et al. 2019

Advanced Optical Materials

View full text Add to dashboard Cite

be used for flexible touch screens and displays, printable electronics, and thinfilm photovoltaics, [4] while FETs made of graphene can be used for highly sensitive biosensors. [5] Another 2D material MoS 2 , a direct-bandgap semiconductor, has applications in ultrasensitive photodetectors, transistors, and gas-sensing devices. [6][7][8][9] Other 2D materials have also shown potential in photovoltaic devices, memory, and light-emitting diodes (LEDs). [10,11] Broad application potential of 2D materials in optoelectronic devices, photonic devices, and optical sensors is mainly based on their unique optical performances. [12] 2D materials can be fabricated based on their layered structure. In the molecular structures of these materials, the atoms are bonded hard in the same plane, but the bond effect between two lateral layers is weak due to van der Waals forces. [13] 2D materials can be roughly categorized as semimetal like graphene, semiconductor like transition metal dichalcogenides (TMDs), and insulator like hexagonal boron nitride (hBN) from the aspect of bandgap differences. [14] Due to the experimentally practical manipulation of monolayer and multilayer 2D materials, remarkable optical performances can be realized for a wide range of optical applications. For example, the number of layers of 2D materials can strongly influence the photoluminescence performance. [15] The unconventional optical performances including adsorption and emission, light sensitivity, as well as plasmonic effects of 2D materials are closely related to their inner physical properties such as carrier mobility, density of states, and band structure based on the interaction of atoms, structural symmetry, and stacking patterns. [16,17] Through the control of layer number which could influence the bandgap value, 2D materials could react to different spectrum ranging from ultraviolet to infrared light. [18] Doping strategies are also effective to modify intrinsic 2D semiconductors and improve the response with respect to an extended range of spectrum. [19,20] The assessment of the optical properties of 2D materials is indispensable for device and sensor development. [21] Based on detailed surface mapping and spectral information, the suitability of 2D materials for optical devices or sensor applications can be thoroughly studied. [22,23] Microscopy, direct 2D mapping of materials, can provide basic spatial information, [24] while spectroscopy can offer one more dimensional spectral information which is 2D materials have unique optical properties due to their ultrathin layered structures, and are emerging as promising materials for optoelectronic devices. To characterize and evaluate these properties, diffraction-limited spectroscopic mapping, also known as microscale spectroscopic imaging, has been developed to provide abundant spatial and spectral information. The usefulness of microscale spectroscopic mapping for unique property study of 2D optical materials is addressed. Advances in both mature and growing microscale spectroscopic mapping...

show abstract

“…Schnell et al ( 35 ) has improved the spatial resolution of IR imaging by coupling a visible light-emitting diode (LED) into the system and using low-coherence interferometry to detect the surface deformation. Samolis et al ( 60 ) showed improved MIP image contrast with the readout from lock-in amplifier phase channels and imaged protein distribution inside fibroblast cells embedded in collagen matrix ( 61 ). Shi et al ( 31 ) recently demonstrated mid-IR imaging of tissue with a spatial resolution of 260 nm by using ultraviolet (UV)–excited photoacoustic as detection method.…”

Section: Introductionmentioning

confidence: 99%

Bond-selective imaging by optically sensing the mid-infrared photothermal effect

2021

View full text Add to dashboard Cite

Mid-infrared (IR) spectroscopic imaging using inherent vibrational contrast has been broadly used as a powerful analytical tool for sample identification and characterization. However, the low spatial resolution and large water absorption associated with the long IR wavelengths hinder its applications to study subcellular features in living systems. Recently developed mid-infrared photothermal (MIP) microscopy overcomes these limitations by probing the IR absorption–induced photothermal effect using a visible light. MIP microscopy yields submicrometer spatial resolution with high spectral fidelity and reduced water background. In this review, we categorize different photothermal contrast mechanisms and discuss instrumentations for scanning and widefield MIP microscope configurations. We highlight a broad range of applications from life science to materials. We further provide future perspective and potential venues in MIP microscopy field.

show abstract

Phase-sensitive lock-in detection for high-contrast mid-infrared photothermal imaging with sub-diffraction limited resolution

Cited by 48 publications

References 42 publications

Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Microscale Spectroscopic Mapping of 2D Optical Materials

Bond-selective imaging by optically sensing the mid-infrared photothermal effect

Contact Info

Product

Resources

About