Phase II randomized clinical trial of LC9018 concurrently used with radiation in the treatment of carcinoma of the uterine cervix. Its effect on tumor reduction and histology

Okawa, Tomohiko; Kita, Midori; Arai, Tatsuo; Iida, K; Dokiya, Takushi; Takegawa, Yoshihiro; Hirokawa, Yutaka; Yamazaki, Kazuto; Hashimoto, S

doi:10.1002/1097-0142(19891101)64:9<1769::aid-cncr2820640902>3.0.co;2-g

Cited by 35 publications

(11 citation statements)

References 14 publications

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“…1980). Moreover, use of LAB in patients with cervical cancer demonstrated a clear effect on tumour reduction compared with radiotherapy alone (Okawa et al . 1989).…”

Section: Resultsmentioning

confidence: 99%

Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Zabala

Martín

Haza

et al. 2001

Lett Appl Microbiol

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Aims: Twenty lactic acid bacteria strains were isolated from human faeces and tested by MTT assay for stimulation or inhibition of the proliferation of Vero and myeloma cells. Methods and Results: None of the strains significantly affected the proliferation of Vero cells. However, two isolates (HN1 and HA8) showed a strong inhibition of myeloma cell proliferation (16·7 and 5·0%, respectively) by MTT assay. Conclusions: Both strains have an anti‐proliferative effect on a tumoral cell line. Significance and Impact of the Study: The beneficial effect of LAB cancer therapy has been linked to their ability for immunomodulation.

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“…1980). Moreover, use of LAB in patients with cervical cancer demonstrated a clear effect on tumour reduction compared with radiotherapy alone (Okawa et al . 1989).…”

Section: Resultsmentioning

confidence: 99%

Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Zabala

Martín

Haza

et al. 2001

Lett Appl Microbiol

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“…4, 5), suggesting that inhibition of the growth of pathogenic bacteria resulted in the suppression of subsequent infection-induced inflammatory responses. L. casei Shirota reportedly exerts antitumor (4,32,45,54) and antimicrobial (47,52,53,70) activities in clinical and preclinical studies. Furthermore, nonspecific augmentation of components of the innate immune system, such as macrophages (34,47,63) and natural killer cells (33), has been thought to play important roles in these activities.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Intraurethrally Administered ProbioticLactobacillus caseiin a Murine Model ofEscherichia coliUrinary Tract Infection

Asahara

Nomoto

Watanuki

et al. 2001

Antimicrob Agents Chemother

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The antimicrobial activity of the intraurethrally administered probiotic Lactobacillus casei strain Shirota against Escherichia coli in a murine urinary tract infection (UTI) model was examined. UTI was induced by intraurethral administration of Escherichia coli strain HU-1 (a clinical isolate from a UTI patient, positive for type 1 and P fimbriae), at a dose of 1 ؋ 10 6 to 2 ؋ 10 6 CFU in 20 l of saline, into a C3H/HeN mouse bladder which had been traumatized with 0.1 N HCl followed immediately by neutralization with 0.1 N NaOH 24 h before the challenge infection. Chronic infection with the pathogen at 10 6 CFU in the urinary tract (bladder and kidneys) was maintained for more than 3 weeks after the challenge, and the number of polymorphonuclear leukocytes and myeloperoxidase activity in the urine were markedly elevated during the infection period. Urinary tract infection (UTI) is the most common bacterial infection seen in clinical practice. Human UTI comprises disease entities such as acute pyelonephritis with renal parenchymal involvement, cystitis limited to the urinary bladder, and asymptomatic bacteruria. Enterobacteriaceae such as Escherichia coli, which are normal inhabitants of human intestines, account for the vast majority of these uncomplicated infections (37,65). Appropriate hygiene and cleanliness of the genital area are therefore recommended for prevention of UTI. On the other hand, studies have shown a correlation between a loss or disruption of the normal genital microflora, in particular Lactobacillus species, and an increased incidence of genital and bladder infections (57). Preclinical and clinical reports have focused on lactobacillus strains, their possible prophylactic effects against experimental E. coli infection, and the use of these strains for the prevention of human urogenital infections (7,12,17,59,60).Suitable animal experimental models are required for appropriate preclinical studies of UTIs. Hagberg et al. were the first to show that mice could be challenged intravesically (by introducing pathogens directly into the bladder) without further manipulations of the urinary tract (18), and the murine model of ascending pyelonephritis has served as an excellent tool for defining the roles of individual virulence factors in the pathogenesis of UTI (18,23,25,26,28,61). It should be noted, however, that the inoculum doses used in murine models are very high (10 8 CFU). Furthermore, high bladder infection levels reportedly persisted over the 14-day study period only in C3H/HeJ and C3H/OuJ mice, which are lipopolysaccharide (LPS) nonresponder strains, while strains such as C3H/HeN, C57BL/6, BALB/c, DBA.1, DBA.2, and AKR showed progressive resolution of bladder infections over a 14-day period (23,24). Therefore, an appropriate model in which chronic UTI can be induced with a lower inoculum of E. coli, regardless of differences in genetic backgrounds, is needed.In the present report, we first describe an improved murine chronic infection model of UTI, in which the infection was induced by tr...

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“…Nevertheless, some early reports have suggested that heat-killed Lactobacillus casei Shirota could be useful in the treatment of carcinoma of the uterine cervix (44, 45) and secondary to lung cancer (46). In one preclinical study, viable L. casei was found to be more effective than heat-killed L. casei in the prevention of secondary tumours in preimmunized mice (47).…”

Section: Clinical Benefits Of Probiotics – Is Viability Essential?mentioning

confidence: 99%

Probiotic viability – does it matter?

Lahtinen¹

2012

Microbial Ecology in Health & Disease

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Phase II randomized clinical trial of LC9018 concurrently used with radiation in the treatment of carcinoma of the uterine cervix. Its effect on tumor reduction and histology

Cited by 35 publications

References 14 publications

Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Antimicrobial Activity of Intraurethrally Administered ProbioticLactobacillus caseiin a Murine Model ofEscherichia coliUrinary Tract Infection

Probiotic viability – does it matter?

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