Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Zabala, Adriana; Martín, Morales; Haza, Ana I.; Fernández, Leónides; Rodríguez, Juan M.; Morales, Paloma

doi:10.1046/j.1472-765x.2001.00910.x

Cited by 24 publications

(10 citation statements)

References 13 publications

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“…At bacterial concentrations of 10 6 -10 7 cfu/mL, a weak inhibition of cellular proliferation (70.9 ± 81.5% of survival) was observed for both strains. However, they displayed a strong inhibitory effect (16.7% and 5% for E. faecium HN1 and L. salivarius HA8, respectively) at a concentration of 10 8 cfu/mL (Zabala et al, 2001). We also observed a dose-dependent response for the antiproliferative abilities of P. pentosaceus, L. plantarum, and W. confusa in our study.…”

Section: Discussionsupporting

confidence: 67%

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Er¹,

Koparal²,

Kıvanç³

2015

Turk J Biol

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Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to investigate the antiproliferative effects of the cell-free filtrate and the cell-free lyophilized filtrate of 3 LAB (Pediococcus pentosaceus, Lactobacillus plantarum, and Weissella confusa) on human colorectal adenocarcinoma cell line Caco-2. The filtrates were found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that these strains might have use as probiotics in functional food or for colon cancer treatment. There are no other studies related to the anticancer activities of W. confusa in the literature.

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Section: Discussionsupporting

confidence: 67%

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Er¹,

Koparal²,

Kıvanç³

2015

Turk J Biol

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“…Leblanc et al 2005;Matsuzaki et al 2007). This activity has been related to the immunomodulatory activity they possess, and likely on the basis of the changes in the cytokine profiles they induce (Zabala et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma protective response

Castro

Molina

Sciullo

et al. 2010

Journal of Applied Microbiology

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Aims: To study the anti‐tumour effects of Enterococcus faecalis CECT7121 on LBC cells, an aggressive murine T‐cell lymphoma that kills the host in 18 days when is intraperitoneally (i.p.) administrated. Methods and Results: In vitro studies have shown that LBC cell proliferation was inhibited by Ent. faecalis CECT7121 stimulus in a dose‐dependent manner, inducing apoptosis. The production of ceramide was involved in the latter effect. To undertake in vivo studies, syngeneic BALB/c mice pre‐treated i.p. with Ent. faecalis CECT7121 (2·5 × 108 CFU) were challenged i.p. with LBC cells (1·0 × 106 cells) the day after. On day 30 post‐inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre‐treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re‐challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNγ, IL‐12 and IL‐10 levels were observed in surviving animals. Conclusions: Enterococcus faecalis CECT7121 affected multiple factors of the tumour establishment by the following methods: down‐regulating the LBC cell proliferation and inducing apoptosis in these cells; and enhancing the immune response that protects animals from lymphoma challenge and re‐challenge. Significance and Impact of the Study: This study demonstrate that Ent. faecalis CECT7121 has potential as a probiotic that could facilitate the development of novel complements to therapeutic strategies against oncological diseases.

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“…In a previous report, we showed that there was an antiproliferative effect of L. plantarum , Enterococcus faecium and L. salivarius strains isolated from ewe's milk cheese (Haza et al. , 2004) and human faeces (Zabala et al. , 2001a,b).…”

Section: Discussionmentioning

confidence: 99%

The inhibition of the viability of myeloma cells and the production of cytokine by two strains of Lactobacillus sakei from meat

Haza

Zabala

Arranz

et al. 2005

Int J of Food Sci Tech

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Ten strains of lactic acid bacteria were isolated from a dry fermented sausage and tested for stimulation or inhibition of the viability of Vero and myeloma cells. They did not significantly affect the viability of Vero cells but two isolates (CBL/H and CBL/K) showed a strong and moderate inhibition of the myeloma cell viability (at 10 8 CFU mL )1 , 17.6 and 33.2%, respectively, survival of myeloma cells). The isolates were identified as Lactobacillus sakei by DNA sequencing of the 16S rRNA products of a polymerase chain reaction. No protective effect was found, at the concentrations used, against cytotoxicity of N-nitrosamines. To test the effect of L. sakei CBL/H and CBL/K on cytokine production [tumour necrosis factor alpha (TNF-a), interleukin-1b (IL-1b) and interleukin-8 (IL-8)], the human macrophage cell line (THP-1) was cultured in the presence and absence of lipopolysaccharide (LPS). Lactobacillus sakei CBL/H and CBL/K induced IL-1b and IL-8 release when cells were stimulated with and without LPS. However there was TNF-a release only in the presence of the LPS.

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Anti-proliferative effect of two lactic acid bacteria strains of human origin on the growth of a myeloma cell line

Cited by 24 publications

References 13 publications

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma protective response

The inhibition of the viability of myeloma cells and the production of cytokine by two strains of Lactobacillus sakei from meat

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