Phase-Contrast OCT Imaging of Transverse Flows in the Mouse Retina and Choroid

Fingler, Jeff; Readhead, Carol; Schwartz, Daniel M.; Fraser, Scott E.

doi:10.1167/iovs.07-1627

Cited by 77 publications

(45 citation statements)

References 8 publications

(12 reference statements)

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“…48 Figure 9 shows two pvOCM images acquired sequentially over exactly the same trunk area, separated in time by ∼90 s. Red arrows mark locations that have the phase variance contrast in one image but not the other, validating our speculation that the missing contrast was caused by the intrinsic pulsatile nature of blood flow. Combining data from two or more pvOCM images (either a maximum or an average value rendering for each pixel in the images) resulted in a more complete rendering of the blood flow.…”

Section: Discussionmentioning

confidence: 52%

Phase variance optical coherence microscopy for label-free imaging of the developing vasculature in zebrafish embryos

2016

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, "Phase variance optical coherence microscopy for labelfree imaging of the developing vasculature in zebrafish embryos," J. Abstract. A phase variance optical coherence microscope (pvOCM) has been created to image blood flow in the microvasculature of zebrafish embryos, without the use of exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2.8 μm in tissue and imaging depth of more than 100 μm. Images of 2 to 5 days postfertilization zebrafish embryos identified the detailed anatomical structure based on OCM intensity contrast. Phase variance contrast offered visualization of blood flow in the arteries, veins, and capillaries. The pvOCM images of the vasculature were confirmed by direct comparisons with fluorescence microscopy images of transgenic embryos in which the vascular endothelium is labeled with green fluorescent protein. The ability of pvOCM to capture activities of regional blood flow permits it to reveal functional information that is of great utility for the study of vascular development. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Discussionmentioning

confidence: 52%

Phase variance optical coherence microscopy for label-free imaging of the developing vasculature in zebrafish embryos

2016

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“…The PV-OCT method 47,[51][52][53][54][55][56][57][58][59][60][61][62][63][64][65][66] has been implemented to visualize the vasculature of zebrafish, 59 ocular circulation of the mouse, 60 and human retinal vasculature. 47,[61][62][63][64][65][66] This method identifies the phase difference between consecutive B-scans of the same transverse position and allows for the mapping of microvasculature, independent of the direction of flow relative to the imaging system.…”

Section: Introductionmentioning

confidence: 99%

“…47,[61][62][63][64][65][66] This method identifies the phase difference between consecutive B-scans of the same transverse position and allows for the mapping of microvasculature, independent of the direction of flow relative to the imaging system. 47,60 High-speed phase stable imaging systems and postimage processing can reduce motion artifacts, which may further advance this technique for use in clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Review of speckle and phase variance optical coherence tomography to visualize microvascular networks

et al. 2013

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Abstract. High-resolution mapping of microvasculature has been applied to diverse body systems, including the retinal and choroidal vasculature, cardiac vasculature, the central nervous system, and various tumor models. Many imaging techniques have been developed to address specific research questions, and each has its own merits and drawbacks. Understanding, optimization, and proper implementation of these imaging techniques can significantly improve the data obtained along the spectrum of unique research projects to obtain diagnostic clinical information. We describe the recently developed algorithms and applications of two general classes of microvascular imaging techniques: speckle-variance and phase-variance optical coherence tomography (OCT). We compare and contrast their performance with Doppler OCT and optical microangiography. In addition, we highlight ongoing work in the development of variance-based techniques to further refine the characterization of microvascular networks. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…We have developed a vascular visualization technique called phase variance contrast optical coherence tomography (PV-OCT), which identifies regions of mobile scatterers based upon the variance of phase changes. This contrast method has previously been demonstrated in several different imaging scenarios: visualizing vasculature of the zebrafish [2], retinal vasculature of the mouse [3], and identifying microvasculature in small regions of the human retina [4]. This manuscript will focus on the visualization capabilities and limitations of PV-OCT and the applicability for vascular imaging over large retinal areas using a SDOCT system with an acquisition speed of 25 kHz, a speed comparable to current commercially-available retinal OCT systems.…”

Section: Introductionmentioning

confidence: 99%

Extended volume retinal vascular imaging with phase variance contrast optical coherence tomography

Fingler

Schwartz

Fraser

2011

Ophthalmic Technologies XXI

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We present recent developments from a phase variance based motion contrast method of retinal vascular OCT imaging, called phase variance contrast optical coherence tomography (PV-OCT). Using a 25 kHz spectral domain optical coherence tomography (SDOCT) system, the vascular visualization capabilities of this contrast method are demonstrated with composite images created from multiple data sets. Wide field vascular images extending over the fovea and optic nerve head are presented as well as microvascular retinal images over the fovea to demonstrate the trade-offs between imaging speed and vascular visualization.

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Phase-Contrast OCT Imaging of Transverse Flows in the Mouse Retina and Choroid

Abstract: Phase-contrast OCT enables three-dimensional visualization of retinal and choroidal vasculature in vivo.

Cited by 77 publications

References 8 publications

Phase variance optical coherence microscopy for label-free imaging of the developing vasculature in zebrafish embryos

Phase variance optical coherence microscopy for label-free imaging of the developing vasculature in zebrafish embryos

Review of speckle and phase variance optical coherence tomography to visualize microvascular networks

Extended volume retinal vascular imaging with phase variance contrast optical coherence tomography

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