Phase-contrast microtomography using an X-ray interferometer having a 40-μm analyzer

Momose, Atsushi; Koyama, Ichiro; Hamaishi, Yoshitaka; Yoshikawa, Hiroshi; Takeda, Takeshi; Wu, Jin; Itai, Yuji; Takai, Kengo; Uesugi, K.; Suzuki, Yoshio

doi:10.1051/jp4:20030153

Cited by 18 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phase-contrast X-ray imaging system was previously described in detail (19,38). It consists of a monolithic X-ray interferometer, a phase shifter, an object cell, and an X-ray detector.…”

Section: Methodsmentioning

confidence: 99%

Atherosclerotic plaque imaging using phase-contrast X-ray computed tomography

Shinohara

Yamashita

Tawa

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

Reliable, noninvasive imaging modalities to characterize plaque components are clinically desirable for detecting unstable coronary plaques, which cause acute coronary syndrome. Although recent clinical developments in computed tomography (CT) have enabled the visualization of luminal narrowing and calcified plaques in coronary arteries, the identification of noncalcified plaque components remains difficult. Phase-contrast X-ray CT imaging has great potentials to reveal the structures inside biological soft tissues, because its sensitivity to light elements is almost 1,000 times greater than that of absorption-contrast X-ray imaging. Moreover, a specific mass density of tissue can be estimated using phase-contrast X-ray CT. Ex vivo phase-contrast X-ray CT was performed using a synchrotron radiation source (SPring-8, Japan) to investigate atherosclerotic plaque components of apolipoprotein E-deficient mice. Samples were also histologically analyzed. Phase-contrast X-ray CT at a spatial resolution of 10 -20 m revealed atherosclerotic plaque components easily, and thin fibrous caps were detected. The specific mass densities of these plaque components were quantitatively estimated. The mass density of lipid area was significantly lower (1.011 Ϯ 0.001766 g/ml) than that of smooth muscle area or collagen area (1.057 Ϯ 0.001407 and 1.080 Ϯ 0.001794 g/ml, respectively). Moreover, the three-dimensional assessment of plaques could provide their anatomical information. Phasecontrast X-ray CT can estimate the tissue mass density of atherosclerotic plaques and detect lipid-rich areas. It can be a promising noninvasive technique for the investigation of plaque components and detection of unstable coronary plaques. synchrotron radiation; atherosclerotic plaque component; tissue-mass density of plaque component ATHEROSCLEROSIS IS A SYSTEMIC disease in which the clinical manifestations weakly correlate with its extent and with the severity of luminal stenosis. Coronary plaque disruption and subsequent thrombosis in coronary atherosclerosis lead to a potentially life-threatening disease, acute coronary syndrome (11,15). Clinical evidence suggests that atherosclerotic plaque components are important predictors of plaque stability and clinical events. The risk of plaque rupture appears to depend on the plaque components rather than the severity of stenosis.Most ruptures occur in "unstable" plaques containing a soft lipid-rich core, covered by a thin and inflamed cap of fibrous tissue (39).Invasive techniques, such as coronary angiography, intravascular ultrasound, and optical coherent tomography, can reveal the luminal diameter or stenosis, wall thickness, and plaque volume and components (16). However, to detect an unstable plaque that leads to acute coronary syndrome, reliable, noninvasive imaging modalities for the characterization of plaque components are clinically desirable. Currently, two emerging and promising techniques, namely, computed tomography (CT) and magnetic resonance imaging (MRI), are widely accepted by the medic...

show abstract

Section: Methodsmentioning

confidence: 99%

Atherosclerotic plaque imaging using phase-contrast X-ray computed tomography

Shinohara

Yamashita

Tawa

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…To achieve higher spatial resolution imaging, Momose et al developed a triple Laue-case (LLL) X-ray interferometer with a 0.04-mm thick analyzer [16,17]. In this study, we performed the comparative study on the micro-phase-contrast CT images in contrast with the optical microscopic images as confirmation of the applicability of this technique for basic biomedical researches.…”

Section: Introductionmentioning

confidence: 82%

“…In addition, the high spatial resolution as by micro x-ray imaging leads to the less integrated linear attenuation in target regions, and this also induces the difficulty in detecting the minute density difference of the inner structures on the object. On the other hand, the phase-contrast X-ray imaging technique has high sensitive to depict the inner soft tissue structures of human and animals [6][7][8][9][10][11][12][13], and recently we can observe the biological object with higher spatial resolution by the micro-phase-contrast CT [16,17,25].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

Takeda

Lwin

et al. 2004

SPIE Proceedings

View full text Add to dashboard Cite

Micro-phase-contrast X-ray computed tomography with an X-ray interferometer (micro-phase-contrast CT) is in operation to obtain high spatial resolution images of less than 0.01 mm at the undulator beam-line 20XU of SPring-8, Japan, and we applied micro-phase-contrast CT to observe the organs of rats and hamsters. The excised kidney and spleen fixed by formalin were imaged. The fine inner-structures such as vessels, glomeruli of kidney and white and red pulps of spleen were visualized clearly about 0.01-mm spatial resolutions without using contrast agent or staining procedure. The results were very similar to those by optical microscopic images with 20-fold magnification. These results suggest that the micro-phase tomography might be a useful tool for various biomedical researches.

show abstract

“…Its high sensitivity to the structures in biological soft tissues is noteworthy [2,[4][5][6] even compared with other phasesensitive methods [9]. The details of the interferometer and an experimental setup were described elsewhere [8]. In order to reduce the effect, a LLL X-ray interferometer having a 40-µm lamella, which was conventionally 1 mm, was fabricated.…”

Section: Phase Tomography With Crystal X-ray Interferometermentioning

confidence: 99%

Development of Apparatus for Phase Imaging Using X-Ray Interferometers at BL20XU of SPring-8

Momose¹

2004

AIP Conference Proceedings

Self Cite

View full text Add to dashboard Cite

Phase-contrast X-ray imaging, including phase tomography, was performed at the medium-length beamline BL20XU of SPring-8, where undulator X rays were available at 245 m from the source point. A crystal X-ray interferometer and an X-ray Talbot interferometer with transmission gratings were used to perform different kinds of phasecontrast imaging. The crystal X-ray interferometer was devised in that a crystal lamella for beam recombination was thinned down to 40 µm to achieve high-resolution imaging. A quick fringe-scanning mechanism was also constructed enabling the phase measurement with a few second and therefore phase tomography with several minutes. The X-ray Talbot interferometer was studied as a novel and simple method that generated a contrast corresponding to differential phase. Phase tomography was also attained with the X-ray Talbot interferometer. FIGURE 1.Three-dimensional images obtained by phase tomography: (a) an image of a tissue of rat kidney obtained with 0.1nm X rays using an X-ray interferometer having a 40-µm lamella, (b) an image of a tissue of human kidney obtained with 0.07nm X rays [11] using an X-ray interferometer having a 1-mm lamella.

show abstract

Phase-contrast microtomography using an X-ray interferometer having a 40-μm analyzer

Cited by 18 publications

References 0 publications

Atherosclerotic plaque imaging using phase-contrast X-ray computed tomography

Atherosclerotic plaque imaging using phase-contrast X-ray computed tomography

Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

Development of Apparatus for Phase Imaging Using X-Ray Interferometers at BL20XU of SPring-8

Contact Info

Product

Resources

About