Pharmacological studies of the large-scaled purified genistein from Huaijiao (Sophora japonica – Leguminosae) on anti-osteoporosis

Wang, Z.L.; Sun, Jianfeng; Wang, D.N.; Xie, Youhua; Wang, S.W.; Zhao, Wenming

doi:10.1016/j.phymed.2005.09.005

Cited by 42 publications

(28 citation statements)

References 9 publications

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“…In addition, apigenin (8), quercitrin (9), and rutin (10) were also isolated from this plant. Isolation of all of the above nine compounds, but quercitrin (quercetin-3-Orhamnoside), was previously reported from this plant (Takeda et al, 1977;Tang et al, 2008;Wang et al, 2003;Wang et al, 2006). The effects of compounds 1-10 on rat platelet aggregation induced by AA (10-30 µM), collagen (2-3 µg/mL), U46619 (2-5 µM), and ADP (2-5 µM) were evaluated, and compared with those of acetyl salicylic acid (ASA), a well-known inhibitor of platelet aggregation (Table I).…”

Section: Resultsmentioning

confidence: 93%

“…Its buds and fruits have been used as haemostatic agents in traditional Korean medicine, and flavonoids from the buds were reported as haemostatic constituents (Ishida et al, 1989). Flavonol tetraglycosides (Wang et al, 2003), triterpene glycosides (Gorbacheva et al, 1996), phospholipids, alkaloids, amino acids, and polysaccharides (Grishkovets and Gorbacheva, 1995;Mukahamedova and Glushenkova, 1997;Sun et al, 2007) from its seeds, and isoflavones and isoflavone tetraglycosides from its small branches (Min et al, 2006;Tang et al, 2008;Wang et al, 2006) have been isolated. Several flavonoids and their glycosides, acting as anti-inflammatory components have also been isolated (Kim et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Kim

Yun‐Choi

2008

Arch. Pharm. Res.

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A methanol extract of Sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin A (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). The structure of each compound was determined by a variety of spectroscopic methods. Among the compounds, 1, 3, and 7 showed approximately 2.5-6.5 fold greater inhibitory effects on arachidonic acid (AA) and U46619 induced platelet aggregation (IC50: 19.9 and 99.8 microM; 20.3 and 53.8 microM; 25.9 and 123.4 microM, respectively) than acetylsalicylic acid (ASA, IC50: 63.0 and 350.0 microM). Compound 2 was an approximately 22-40 fold stronger inhibitor than ASA on AA and U46619 induced aggregation (IC50: 1.6 and 15.6 microM, respectively).

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Kim

Yun‐Choi

2008

Arch. Pharm. Res.

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“…Genistein from S. japonica was also reported to prevent osteoporosis [13]. Moreover, in-vivo studies have shown that S. japonica extracts prevented bone loss, partly by inhibiting osteoclastic activity [14].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Antiosteoporotic Compounds from Seeds of Sophora japonica

et al. 2014

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Chemical investigation of Sophora japonica seeds resulted in the isolation of seven metabolites identified as: genistin (1), sophoricoside (2), sophorabioside (3), sophoraflavonoloside (4), genistein 7,4’-di-O-β-D-glucopyransoide (5), kaempferol 3-O-α–L-rhamnopyranosyl(1→6)β-D-glucopyranosyl(1→2)β-D-glucopyranoside (6) and rutin (7). Compounds 1, 2 and 5 showed significant estrogenic proliferative effect in MCF-7 cell in sub-cytotoxic concentration range. Compounds 1 and 2 showed minimal cell membrane damaging effect using LDH leakage assay. Accordingly, compound 2 (sophoricoside, (SPH)) was selected for further in-vivo studies as a potential anti-osteoporosis agent. The anti-osteoporotic effect of SPH was assessed in ovarectomized (OVX) rats after oral administration (15 mg/kg and 30 mg/kg) for 45 days compared to estradiol (10 µg/kg) as a positive control. Only in a dose of 30 mg/kg, SPH regained the original mechanical bone hardness compared to normal non-osteoporotic group. However, SPH (15 mg/kg) significantly increased the level of alkaline phosphatase (ALP) to normal level. Treatment with SPH (30 mg/kg) increased the level of ALP to be higher than normal group. SPH (15 mg/kg) did not significantly increase the serum level of osteocalcin (OC) compared to OVX group. On the other hand, treatment with SPH (30 mg/kg) significantly increased the level of OC to 78% higher than normal non-ovarectomized animals group. In addition, SPH (15 mg/kg) decreased the bone resorption marker, acid phosphatase (ACP) to normal level and SPH (30 mg/kg) further diminished the level of serum ACP. Histopathologically, sophoricoside ameliorated the ovarectomy induced osteoporosis in a dose dependent manner. The drug showed thicker bony trabeculae, more osteoid, and more osteoblastic rimming compared to OVX group.

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“…Genistein can be used as a medicine for the prevention of osteoporosis. It also possesses various activities such as anticancer, reducing blood fat, anti-oxidation and estrogen effect [5][6][7]. Research reports also indicated that it can be used to improve certain symptoms of menopause women.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Fructus Sophorae Genistein and Physicochemical Properties of its Complex with Lecithin

Brad

Zhang

2017

MOJDDT

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Pharmacological studies of the large-scaled purified genistein from Huaijiao (Sophora japonica – Leguminosae) on anti-osteoporosis

Cited by 42 publications

References 9 publications

Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Isolation of Antiosteoporotic Compounds from Seeds of Sophora japonica

Extraction and Purification of Fructus Sophorae Genistein and Physicochemical Properties of its Complex with Lecithin

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