Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Kim, Jeong Mi; Yun‐Choi, Hye Sook

doi:10.1007/s12272-001-1242-1

Cited by 91 publications

(49 citation statements)

References 35 publications

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“…Antibody-mediated inhibition of PDI blocks platelet aggregation in vitro (2,4,5). Quercetin-3-rutinoside has previously been demonstrated to inhibit platelet aggregation induced by collagen or ADP (19,20). In anticipation of in vivo thrombus formation studies in mice, we evaluated the effect of quercetin-3-rutinoside on PAR4-mediated platelet aggregation.…”

Section: Quercetin-3-rutinoside Analogs As Pdi Inhibitorsmentioning

confidence: 99%

Protein disulfide isomerase inhibitors constitute a new class of antithrombotic agents

Jasuja

Passam²,

Kennedy³

et al. 2012

J. Clin. Invest.

262

345

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Thrombosis, or blood clot formation, and its sequelae remain a leading cause of morbidity and mortality, and recurrent thrombosis is common despite current optimal therapy. Protein disulfide isomerase (PDI) is an oxidoreductase that has recently been shown to participate in thrombus formation. While currently available antithrombotic agents inhibit either platelet aggregation or fibrin generation, inhibition of secreted PDI blocks the earliest stages of thrombus formation, suppressing both pathways. Here, we explored extracellular PDI as an alternative target of antithrombotic therapy. A high-throughput screen identified quercetin-3-rutinoside as an inhibitor of PDI reductase activity in vitro. Inhibition of PDI was selective, as quercetin-3-rutinoside failed to inhibit the reductase activity of several other thiol isomerases found in the vasculature. Cellular assays showed that quercetin-3-rutinoside inhibited aggregation of human and mouse platelets and endothelial cell-mediated fibrin generation in human endothelial cells. Using intravital microscopy in mice, we demonstrated that quercetin-3-rutinoside blocks thrombus formation in vivo by inhibiting PDI. Infusion of recombinant PDI reversed the antithrombotic effect of quercetin-3-rutinoside. Thus, PDI is a viable target for small molecule inhibition of thrombus formation, and its inhibition may prove to be a useful adjunct in refractory thrombotic diseases that are not controlled with conventional antithrombotic agents.

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Section: Quercetin-3-rutinoside Analogs As Pdi Inhibitorsmentioning

confidence: 99%

Protein disulfide isomerase inhibitors constitute a new class of antithrombotic agents

Jasuja

Passam²,

Kennedy³

et al. 2012

J. Clin. Invest.

262

345

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“…We tested many plant extracts in a search for new compounds that promoted POMC or CART expression for the de- Apigenin (4',5,7-trihydroxyflavone) is a common dietary flavonoid found in many fruits and vegetables, including parsley, onion, orange, tea, chamomile, and wheat sprouts, and is present in some seasonings. Apigenin is used as a food supplement and possesses antigenotoxic (Siddique et al, 2008;Siddique and Afzal, 2009), anti-inflammatory (Ko et al, 2004;Yin et al, 2008), antiplatelet (Kim and Yun-Choi, 2008), and antitumor effects (Fotsis et al, 1997;Gupta et al, 2001;Patel et al, 2007). However, the anorexigenic effects of apigenin have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Apigenin isolated from the seeds of Perilla frutescens britton var crispa (Benth.) inhibits food intake in C57BL/6J mice

Myoung¹,

Kim²,

Nam

2010

Arch. Pharm. Res.

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Energy balance is monitored by the hypothalamus, which responds to peripheral signals by releasing neuropeptides that regulate energy intake and expenditure. In this study, we constructed pro-opiomelanocortin (POMC) and "cocaine and amphetamine-related transcript" (CART) promoter-driven luciferase plasmids and transformed them permanently into both N29-2 neuronal cells and human SHSY5Y cells. Using reporter gene assays, we identified apigenin from the seeds of Perilla frutescens Britton var crispa (Benth.) using activity-guided fractionation. The 50% promoting concentrations (EC₅₀) of apigenin on POMC and CART were 0.93 μM and 0.67 μM, respectively, in N29-2 cells, without significant cytotoxic effects. Shortterm food intake was decreased in C57BL/6J mice after an intraperitoneal injection of apigenin (10 mg/kg; p < 0.05). Food intake and body weight gain for 30 days were also reduced slightly in mice fed a high-fat diet containing apigenin (0.05%, w/w; p < 0.05). These results indicate that apigenin increased POMC and CART gene expression in neuronal cells and significantly reduced food intake in C57BL/6 mice, which may be related to the anorexigenic neuropeptides POMC and CART.

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“…[63] and I. germanica L. [77]. From the pharmacological point of view, this compound shows several different medicinal properties i.e., antioxidant [36], anti-inflammatory, antiproliferative, antineoplastic [63], antiallergic, anti-platelet [78], hepato and neuroprotective [79,80]. Moreover, it's able to lower the level of glucose in the blood [36] presenting also a nutritional value.…”

Section: Resultsmentioning

confidence: 99%

Isoflavones and Other Compounds from the Roots of Iris marsica I. Ricci E Colas. Collected from Majella National Park, Italy

Venditti¹,

Frezza²,

Rai³

et al. 2017

Med chem (Los Angeles)

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In this study, a phytochemical analysis was performed, for the first time, on Iris marsica I. Ricci e Colas. In particular, the attention was focused on the constituents of the roots. Twenty-one compounds were isolated by column chromatography and were analyzed/identified by NMR spectroscopy and mass spectrometry. They all own chemotaxonomic, ethno-pharmacological and nutraceutical relevance which allowed us to provide a phytochemical rationale, for the correct botanical classification of this species, for the employment of its roots in folk medicine like for all the other species belonging to the Iris genus and, lastly, for their further uses as food with important healthy benefits. All of these parts were broadly discussed about within the text.

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Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Cited by 91 publications

References 35 publications

Protein disulfide isomerase inhibitors constitute a new class of antithrombotic agents

Protein disulfide isomerase inhibitors constitute a new class of antithrombotic agents

Apigenin isolated from the seeds of Perilla frutescens britton var crispa (Benth.) inhibits food intake in C57BL/6J mice

Isoflavones and Other Compounds from the Roots of Iris marsica I. Ricci E Colas. Collected from Majella National Park, Italy

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