Pharmacological Studies of Tentacle Extract from the Jellyfish Cyanea capillata in Isolated Rat Aorta

Wang, Beilei; Zhang, Bo; Wang, Qianqian; Zhang, Zhi; Nie, Fei; Liu, Guoyan; Zheng, Jiemin; Liang, Xiao; Zhang, Liming

doi:10.3390/md11093335

Cited by 13 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A number of publications during 2012–2013 reported extracts or structurally uncharacterized marine compounds, with novel and interesting preclinical and/or clinical pharmacology: in vitro antimalarial activity in crude extracts from Fiji marine organisms using a semi-automated RNA fluorescence-based high-content live cell-imaging assay [10]; the first report of in vitro liver stage antiplasmodial activity and dual stage inhibitory potential of British seaweeds [11]; anti-hepatitis C virus activity affecting the viral helicase NS3 and replication, in crude extracts from the marine feather star Alloeocomatella polycladia [12]; anti-herpes simplex virus HSV-1 and HSV-2 activity in a purified sulfoglycolipid fraction from the Brazilian marine alga Osmundaria obtusiloba [13]; in vivo anti-inflammatory activity of a heterofucan from the Brazilian seaweed Dictyota menstrualis that inhibited leukocyte migration to sites of tissue injury by binding to the cell membrane [14]; in vivo antinociceptive and anti-inflammatory activity in a crude methanolic extract of the red alga Bryothamnion triquetrum [15]; in vivo anti-inflammatory activity in a sulfate polysaccharide fraction from the red alga Gracilaria caudata resulting in significant inhibition of neutrophil migration and cytokine release [16]; in vitro anti-inflammatory effect of a hexane-soluble fraction of the brown alga Laminaria japonica that inhibited nitric oxide, prostaglandin E 2 , interleukin (IL)-1β and IL-6 release from lipopolysaccharide-stimulated macrophages via inactivation of nuclear factor-κB transcription factor [17]; in vivo anti-inflammatory of a polysaccharide-rich fraction from the marine red alga Lithothamnion muelleri that reduced organ injury and lethality, as well as pro-inflammatory cytokines and chemokines, associated with graft-versus-host disease in mice [18]; in vivo clinical effectiveness in an osteoarthritis trial by PCSO-524 TM , a nonpolar lipid extract from the New Zealand marine green lipped mussel Perna canaliculus , which may offer “potential alternative complementary therapy with no side effects for osteoarthritis patients” [19]; enhanced antioxidant activity of chitosan nanoparticles as compared to chitosan on hydrogen peroxide-induced stress injury in mouse macrophages in vitro [20]; induction of concentration-dependent vasoconstrictive activity on isolated rat aorta by a tentacle extract from the jellyfish Cyanea capillata [21]; significant antioxidant effect of a sulfated-polysaccharide fraction of the marine red alga Gracilaria birdiae which prevented naproxen-induced gastrointestinal damage in rats by reversing glutathione depletion [22]; in vitro antioxidant properties of a polysaccharide from the brown seaweed Sargassum graminifolium (Turn.) that was also observed to inhibit calcium oxalate crystallization, a constituent of urinary kidney stones [23]; antioxidant activity in organic extracts from 30 species of Hawaiian marine algae, with the carotenoid fucoxanthin identified as the major bioactive antioxidant compound in the brown alga T. ornata [24]; screening of antioxidant activity in 18 cyanobacteria and 23 microalgae cell extracts identified Scenedesmus obliquus strai...…”

Section: Introductionmentioning

confidence: 99%

Marine Pharmacology in 2012–2013: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

et al. 2017

View full text Add to dashboard Cite

The peer-reviewed marine pharmacology literature from 2012 to 2013 was systematically reviewed, consistent with the 1998–2011 reviews of this series. Marine pharmacology research from 2012 to 2013, conducted by scientists from 42 countries in addition to the United States, reported findings on the preclinical pharmacology of 257 marine compounds. The preclinical pharmacology of compounds isolated from marine organisms revealed antibacterial, antifungal, antiprotozoal, antituberculosis, antiviral and anthelmitic pharmacological activities for 113 marine natural products. In addition, 75 marine compounds were reported to have antidiabetic and anti-inflammatory activities and affect the immune and nervous system. Finally, 69 marine compounds were shown to display miscellaneous mechanisms of action which could contribute to novel pharmacological classes. Thus, in 2012–2013, the preclinical marine natural product pharmacology pipeline provided novel pharmacology and lead compounds to the clinical marine pharmaceutical pipeline, and contributed significantly to potentially novel therapeutic approaches to several global disease categories.

show abstract

Section: Introductionmentioning

confidence: 99%

Marine Pharmacology in 2012–2013: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The venom of the giant jellyfish Nemopilema nomurai , administrated intravenously in rats, induces dose-dependent hypotension and bradycardia (0.1-2.4 mg protein/kg); this venom also produces contraction of isolated rat aortic rings [35]. Tentacle extract from the jellyfish Cyanea capillata induces contraction in both endothelium-intact and endothelium-denuded isolated aortic rings [36]. In other studies, it was found that aqueous extracts from the calcareous hydrozoans M. alcicornis and M. complanata induce vasoconstriction of isolated rat aortic rings [37, 38].…”

Section: Discussionmentioning

confidence: 99%

“…It has been proposed that the presence of PLA 2 enzymes in cnidarian venoms plays an important role in defense against predators, and in the immobilization and digestion of prey [20, 44]. Moreover, some studies have related PLA 2 activity to hemolysis [36, 46]. …”

Section: Discussionmentioning

confidence: 99%

Composition and biological activities of the aqueous extracts of three scleractinian corals from the Mexican Caribbean: Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea

García-Arredondo

Rojas‐Molina

Ibarra‐Alvarado

et al. 2016

J Venom Anim Toxins Incl Trop Dis

View full text Add to dashboard Cite

BackgroundScleractinian corals (stony corals) are the most abundant reef-forming cnidarians found in coral reefs throughout the world. Despite their abundance and ecological importance, information about the diversity of their toxins and their biological activities is very scarce. In this study, the chemical composition and the biological activities of the aqueous extracts of Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea, three scleractinian corals from the Mexican Caribbean, have been assessed for the first time.MethodsToxicity of the extracts was assessed in crickets; the presence of cytolysins was detected by the hemolysis assay; the vasoconstrictor activity was determined by the isolated rat aortic ring assay; the nociceptive activity was evaluated by the formalin test. The presence of phospholipases A2 (PLA2), serine proteases, and hyaluronidases was determined by enzymatic methods. Low-molecular-weight fractions were obtained by gel filtration chromatography and ultrafiltration.ResultsExtracts from the three species were toxic to crickets, induced hemolysis in human and rat erythrocytes, produced vasoconstriction on isolated rat aortic rings, and presented phospholipase A2 and serine-protease activity. Despite the fact that these corals are not considered to be harmless to humans, the extracts generated significant nociceptive responses. The matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of the low-molecular-weight fractions revealed the presence of peptides within a mass range of 3000 to 6000 Da. These fractions were toxic to crickets and two of them induced a transitory vasoconstrictor effect on isolated rat aortic rings.ConclusionThis study suggests that scleractinian corals produce low-molecular-weight peptides that are lethal to crickets and induce vasoconstriction.

show abstract

“…The isolated tentacles were placed in plastic bags with dry ice and immediately shipped to Shanghai, where the samples were stored in a -80 °C freezer until use. The TE preparation procedure has been described in previous reports ( Wang et al 2013a;Wang et al 2013b;Wang et al 2013c;Wang et al 2013d;Zhang et al 2014). Briefly, tentacles at -80 °C were thawed and immersed in seawater (28 g/L NaCl, 5 g/L MgCl 2 •6H 2 O, 0.8 g/L KCl, 1.033 g/L CaCl 2 ) to allow tissue autolysis and stirred gently for 4 days.…”

Section: Preparation Of Tentacle Extract From the Jellyfish C Capillatamentioning

confidence: 99%

Peer Review #1 of "Intervention effects of five cations and their correction on hemolytic activity of tentacle extract from the jellyfish Cyanea capillata (v0.1)"

2017

View full text Add to dashboard Cite

Cations have generally been reported to prevent jellyfish venom-induced hemolysis through multiple mechanisms by spectrophotometry. Little attention has been paid to the potential interaction between cations and hemoglobin, potentially influencing the antagonistic effect of cations. Here, we explored the effects of five reported cations, La 3+ , Mn 2+ , Zn 2+ , Cu 2+ and Fe 2+ , on a hemolytic test system and the absorbance of hemoglobin, which was further used to measure their effects on the hemolysis of tentacle extract (TE) from the jellyfish Cyanea capillata. All the cations displayed significant dose-dependent inhibitory effects on TE-induced hemolysis with various dissociation equilibrium constant (K d) values as follows: La 3+ 1.5 mM, Mn 2+ 93.2 mM, Zn 2+ 38.6 mM, Cu 2+ 71.9 µM and Fe 2+ 32.8 mM. The transparent non-selective pore blocker La 3+ did not affect the absorbance of hemoglobin, while Mn 2+ reduced it slightly. Other cations, including Zn 2+ , Cu 2+ and Fe 2+ , greatly decreased the absorbance with K d values of 35.9 mM, 77.5 mM and 17.6 mM, respectively. After correction, the inhibitory K d values were 1.4 mM, 45.8 mM, 128.5 µM and 53.1 mM for La 3+ , Zn 2+ , Cu 2+ and Fe 2+ , respectively. Mn 2+ did not inhibit TE-induced hemolysis. Moreover, the inhibitory extent at the maximal given dose of all cations except La 3+ was also diminished. These corrected results from spectrophotometry were further confirmed by direct erythrocyte counting under microscopy. Our results indicate that the cations, except for La 3+ , can interfere with the absorbance of hemoglobin, which should be corrected when their inhibitory effects on hemolysis by jellyfish venoms are examined. The variation in the inhibitory effects of cations suggests that the hemolysis by jellyfish venom is mainly attributed to the formation of non-selective cation pore complexes over other potential mechanisms, such as phospholipases A2 (PLA2), polypeptides, protease and oxidation. Blocking the pore-forming complexes may be a primary strategy to improve the in vivo damage and mortality from jellyfish stings due to hemolytic toxicity.

show abstract

Pharmacological Studies of Tentacle Extract from the Jellyfish Cyanea capillata in Isolated Rat Aorta

Cited by 13 publications

References 40 publications

Marine Pharmacology in 2012–2013: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

Marine Pharmacology in 2012–2013: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

Composition and biological activities of the aqueous extracts of three scleractinian corals from the Mexican Caribbean: Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea

Peer Review #1 of "Intervention effects of five cations and their correction on hemolytic activity of tentacle extract from the jellyfish Cyanea capillata (v0.1)"

Contact Info

Product

Resources

About