Pharmacological modulation of fluid secretion in the pigmented rabbit conjunctiva

Nucleotide-Induced Restoration of Conjunctival Chloride and Fluid Secretion in Adenovirus Type 5-Infected Pigmented Rabbit Eyes

“…Net fluid secretion rate (J v ) of 4.70 Ϯ 0.25 l/(h ⅐ cm 2 ) (n ϭ 45) in the s-to-m direction at baseline conditions was not statistically significant from the previously reported value (Shiue et al, 2000). Instillation of 10 M UTP to the mucosal side of the excised conjunctiva resulted in a significant increase in net fluid secretion [⌬J v ϭ 6.02 Ϯ 0.02 l/(h ⅐ cm 2 ) (n ϭ 3, p Ͻ 0.05)] during 10-to 15-min postinstillation.…”

“…The eyeballs were then surgically removed from the socket, and the conjunctival tissues were carefully isolated and trimmed. For fluid secretion studies, the trimmed tissues were mounted on adaptors with a circular aperture of 0.385 cm 2 and placed between two Lucite chambers, as described by Shiue et al (2000). The tissue was bathed on both sides with BRS.…”

“…A pair of capacitance probes (ASP-10-CTA/SP; Mechanical Technology, Inc., Latham, NY) was used to measure the rate of fluid secretion (Shiue et al, 2000). The capacitance probes were equilibrated at 37°C for at least 1 h before the mounting of the tissues to prevent vapor condensation on the surface…”

“…The role of cystic fibrosis transmembrane conductance regulator in active Cl Ϫ transport and concomitant fluid secretion in the presence of 8-Br-cAMP or forskolin in the conjunctiva has been suggested previously (Shiue et al, 2000(Shiue et al, , 2002. Purinergic receptor agonists prove to be very useful in defective cystic fibrosis transmembrane conductance regulator conditions by offering a cAMP-independent, Ca 2ϩ -dependent alternative mechanism of Cl Ϫ and fluid secretion in the conjunctiva.…”

Functional Expression of P2Y Receptors in WERI-Rb1 Retinoblastoma Cells

“…P2Y2 receptors have been identified in a wide variety of ocular cell types, particularly, in retina [26], retinal pigment epithelium [27][28][29], cornea [30,31], conjunctiva [32], lens [33], and ciliary epithelia [9,34]. P2Y2 receptor signaling and its function are apparent in the eye [35], and regulates multiple cellular functions in ocular physiology by calcium mobilization [9] including ion transport and fluid absorption [27,29], and mucin discharge [36].…”