Pharmacological Investigation of the Wound Healing Activity of <i>Cestrum nocturnum</i> (L.) Ointment in Wistar Albino Rats

Nagar, Hemant; Srivastava, Amit; Srivastava, Rajnish; Kurmi, Madan Lal; Chandel, Harinarayan Singh; Ranawat, Mahendra Singh

doi:10.1155/2016/9249040

Cited by 78 publications

(69 citation statements)

References 26 publications

(26 reference statements)

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“…A. maurorum extract and the alginate aqueous solution were used for the MTT assay. Although some of the previous studies directly used the herb extracts (Nagar et al, ) or polymers (Roh et al, ) for in vivo assays, in this research, the nontoxic concentration of each material was determined first. Then, the substance was used for in vivo analyses, in its calculated nontoxic dose.…”

Section: Discussionmentioning

confidence: 99%

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Pourali

Yahyaei

2018

Dermatologic Therapy

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Although alginate has been known to be a good wound dressing, it does not have antimicrobial properties, has low availability, and is expensive. To overcome these problems, the present study was conducted, where the extraction of this material from an available small factory Pseudomonas aeruginosa and the improvement of its wound healing property by its combination with herb extract, Alhagi maurorum, done. Nineteen P. aeruginosa strains were isolated and identified from burned skin, and the one isolated strain with the highest ability of alginate production was selected. A. maurorum aqueous extract was prepared, and the toxicity of each material was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide assay. A mixture of nontoxic doses of each substance was then prepared. Thirty-two Wistar rats were divided into four groups (n = 8). The control group and the rest three groups, which were treated by alginate, A. maurorum extract, and alginate-A. maurorum extract. Throughout the 21 days of treatment, the open wound sites were checked. Finally, the rats were sacrificed and the effect of each substance on their skin tissue was evaluated. The results showed that the high alginate production without any toxic effect was obtained from the P. aeruginosa strain K1. A. maurorum aqueous extract had dose-dependent toxicity. The aqueous solution of alginate-A. maurorum extract complex group showed the best wound healing activity in both macroscopic and microscopic examinations. Recent research has introduced a new type of wound dressing with high wound healing properties. This could decrease the time for re-epithelialization and increase wound contraction percentage. K E Y W O R D Salginate, Alhagi maurorum, Pseudomonas aeruginosa, wound healing

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Section: Discussionmentioning

confidence: 99%

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Pourali

Yahyaei

2018

Dermatologic Therapy

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“…This could be due to in lammatory mediators released from mast cells, causing in lammation and healing of burns. In lammatory mediators (TNF-α, IL-1), platelet-derived growth factor (PDGF) growth factors (TGF-β1), and proteases are released during tissue injury (Nagar et al, 2016).…”

Section: Simple Metal Plate Contact Burn Model Of Wistar Ratmentioning

confidence: 99%

A study on new experimental induction of wound using metal surface contact in a rat model

Balaji

Srinivasan

Prabhu

et al. 2020

ijrps

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Burns injury is a global issue; when burns left unmonitored, it may lead to sepsis & hypovolemia. It majorly contributing to the country's morbidity and mortality. Accidental burns are quite common throughout the world. The degree of burns differs variably, and to identify a possible therapeutic option, it is of great importance for the researchers and scientists. Achieving a uniform burn wound model is thus required to study the burns injury at different levels involving epidermis, dermis, and hypodermis. The goal of this study is to create a simple third-degree contact burn in Wistar albino rats. Third-degree contact burn wound was monitored on 7th, 14th, and 21st day for natural wound healing under gross and histopathological observation. Gross observation reported damaged skin to appear dry, leathery, and charred. Wound closure was observed to be delayed and incomplete on day 21. Histopathology revealed a wound healing process by hematoxylin and eosin method. This metal surface contact method revealed a simplified and cost-effective method for inducing a third degree of a wound in Wistar rats. The study is thus a milestone that could enable research progress in the area of wound healing.

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“…[24][25][26] The hairs on the skin of animals were removed and disinfected using ethanol. 27,28 Then area of wound to be produced was marked with a circular stainless-steel stencil on the dorsal thoracic region on the back of the rats with methylene blue. Using toothed forceps and pointed scis-sors, a circular fragment (300mm 2 in area) in length and 0.2 cm depth of total thick skin was excised.…”

Section: Stability Studymentioning

confidence: 99%

Development of Sheep Butter Based Cream for Dermal Wound Healing

Pratikcha

Das

Ghosh

et al. 2020

IJPI

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Objectives: Sheep butter has been traditionally used in the local villages of upper Himalayan region for its various dermatological benefits. In the current study, sheep butter cream was formulated and evaluated for its wound healing properties for the purpose of scientific validation, standardization, safety, efficacy evaluation. Methods: Nine formulations were developed with varying concentrations of ingredients and evaluated for different parameters. Optimized formulations showing neutral pH, good homogeneity and texture, viscosity, spreadability, consistency and antibacterial activity were selected for evaluation of wound healing property in excision wound model and were compared to standard marketed product. Results: From the results obtained, it was observed that formulated creams enabled easy application and removal from the skin without any evidence of irritation, erythema or leftover residue. Amongst all formulations, F-11, F-12 and F-13 showed the most satisfactory properties and were studied in details. Formulations F-12 and F-13 containing 7.5 g and 10g sheep butter respectively were chosen for evaluation of wound healing activity in excision wound model in albino rats. It was noted that on the 15 th post-wounding day, F-12 and F-13 showed 94.4±0.3% and 96.9±0.6% wound contraction respectively, which were found to be significantly higher when compared with the wound contraction of control group (79.3±7.5%) and was comparable with the standard group. Conclusion: Thus sheep butter appears to be effective agent in the management of wound healing activities.

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Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

Cited by 78 publications

References 26 publications

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

A study on new experimental induction of wound using metal surface contact in a rat model

Development of Sheep Butter Based Cream for Dermal Wound Healing

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