Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis

Xu, Gang; Wu, Shun‐Fan; Gu, Gui-Xiang; Teng, Ziwen; Yè, Gōngyín; Huang, Jia

doi:10.1016/j.ibmb.2017.03.004

Cited by 13 publications

(17 citation statements)

References 99 publications

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“…5). This finding agrees with the general perception of BARs as being preferentially expressed in the neurons of the central nervous system and is consistent with previous studies in insects 89–93 . Most genes showed significantly high expression in the hemolymph tissues (Fig.…”

Section: Discussionsupporting

confidence: 93%

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Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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BACKGROUD Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G‐protein‐coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests. RESULTS In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5‐HT1A, Px5‐HT1B, Px5‐HT2A, Px5‐HT2B, and Px5‐HT7). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2. CONCLUSIONS Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species. © 2021 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 93%

“…7(a)). Therefore, we inferred that PxDOP2 is a functional dopamine receptor with high affinity for dopamine and coupled to G s protein, similar to its insect orthologs 31,90 . The EC 50 value was 3.451 μM for the dopamine‐induced cAMP increase.…”

Section: Discussionmentioning

confidence: 89%

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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“…Since the 1990s, a number of dopamine receptor genes have been identified and isolated from insects including Drosophila melanogaster 14 , 18 , 21 , Apis mellifera 20 , Periplaneta Americana 19 , Ctenocephalides felis 15 , Papilio xuthus 17 , Tribolium castaneum 23 , Chilo suppressalis 24 , 25 and Bombyx mori 26 , 27 . Interestingly, DmDopEcr, a GPCR receptor activated by steroid ecdysone, can be activated by dopamine to increase cAMP levels 28 .…”

Section: Introductionmentioning

confidence: 99%

“…In flies, DmDop2 is expressed in the larval and adult nervous system, and is shown to regulate locomotion 30 . In Lepidoptera, CsDop2 gene in rice stem borer is identified and pharmacologically characterized in the recent report 25 , but the knowledge about D2-like receptors is still lacking. In this study, we cloned D2-like receptor genes from the silkworm, Bombyx mori , a Lepidopteran model, analyzed their evolutionary relationship to metazoan homologs, and identified their function in human embryonic kidney (HEK) 293 cells.…”

Section: Introductionmentioning

confidence: 99%

Two dopamine D2-like receptor genes from the silkworm (Bombyx mori) and their evolutionary history in metazoan

Chen

et al. 2017

Sci Rep

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Dopamine is widely distributed in metazoans and is implicated in many physiological functions. Dopaminergic signaling is mediated through two classes of dopamine receptors, D1-like and D2-like. Phylogeny analysis reveals that, the dopamine receptors probably appeared ahead of the cnidarian divergence, two distinct classes of dopamine receptors likely formed prior to the separation of deuterostomes and protostomes, and INDRs probably split from its ancestor before the emergence of nematodes. Two D2-like genes are closely linked on the same scaffold, and the chromosome region around D2-like gene loci show colinearity among different species within Lepidoptera. These indicate two D2-like and their adjunction genes are likely Lepidoptera-specific orthologs, and occur by gene duplication event taken place after Lepidoptera ancestor split from the common ancestor of Lepidoptera and Diptera. In silkworm, two D2-like genes were expressed in examined tissues, and encoded BmDop2R2 having all the features of D2-like receptors and BmDop2R1 being a truncated variant without the region of N-terminal to TM II. Only dopamine distinctly lowered cAMP levels in BmDop2R2-expressing cells, whereas all tested amines for BmDop2R1 had not markedly effect in pharmacological test. These suggest there is functional difference between the two genes, which are likely resulted from subfunctionalization of gene duplication.

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“…Conventional insecticides have been frequently used as the most popular chemical controls to manage the pests in paddy fields. As a result, pests may become resistant to insecticides, and a number of undesirable impacts on environment, natural enemies and outbreak of secondary pests may occur (Tang et al., ; Xu et al., ). In Iran, six stem borer species are widely distributed in paddy fields, among which the striped stem borer, Chilo suppressalis (Walker) (Lep.…”

Section: Introductionmentioning

confidence: 99%

Inferring climatic controls of rice stem borers’ spatial distributions using maximum entropy modelling

Jalaeian

Golizadeh

Sarafrazi

et al. 2018

J Applied Entomology

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Accurate assessment of pest potential distributions is needed to identify their establishment risks that play a key role in pest management in agricultural ecosystems. We used a correlative niche modelling method (Maxent) to predict and map the spatial distributions of two important rice stem borers, Chilo suppressalis and Sesamia cretica, in paddy fields of Iran. In total, 195 presence occurrence records (101 records for C. suppressalis and 94 records for S. cretica) were compiled. A set of environmental and topographic variables, with the highest effects on the species distributions and the lowest correlations among themselves, were used. The results showed that mainly the northern parts of Iran were the most suitable areas for C. suppressalis, and north, north‐east and south‐west of Iran as the most suitable areas for S. cretica. Both models performed well, with an area under the receiver operating characteristic curve (AUC) of 0.983 and 0.786 for C. suppressalis and S. cretica, respectively. The Maxent models showed higher accuracy for predicting the distribution of the specialist pest with the small range sizes compared to the generalist species. Assessing the importance of environmental variables, which were derived from the jackknife test, showed the precipitation as the variable with the highest contribution (66%) in explaining the spatial distribution of C. suppressalis compared to the other variables. The distribution of S. cretica was influenced by a set of variables derived from both the precipitation and temperature. The Maxent predictions were useful to map the geographical distributions of the risk for both rice stem borers that is needed to develop effective management strategies.

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Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis

Cited by 13 publications

References 99 publications

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Two dopamine D2-like receptor genes from the silkworm (Bombyx mori) and their evolutionary history in metazoan

Inferring climatic controls of rice stem borers’ spatial distributions using maximum entropy modelling

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