Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, <scp><i>Plutella xylostella</i></scp>

Liu, Tiansheng; Zhan, Xue; Yu, Yuan; Wang, Shaozhen; Lu, Cong; Lin, Guifang; Zhu, Xiangyu; He, Weiyi; You, Minsheng; You, Shijun

doi:10.1002/ps.6481

Cited by 9 publications

(14 citation statements)

References 107 publications

(126 reference statements)

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“…Except for AaOAα1-R , all other receptors showed an expression pattern characterized by high levels of transcript in eggs, followed by a decline in larval stages and then high levels in pupae and adults. A similar dynamic expression pattern was observed in Plutella xylostella , in which both OA and TA receptors showed the highest expression levels in eggs and pupae, compared with larvae and adult [ 57 ]. Furthermore, in D .…”

Section: Discussionsupporting

confidence: 54%

Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles

et al. 2023

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In insects, the biogenic amines octopamine (OA) and tyramine (TA) are involved in controlling several physiological and behavioural processes. OA and TA act as neurotransmitters, neuromodulators or neurohormones, performing their functions by binding to specific receptors belonging to the G protein-coupled receptor (GPCR) superfamily. OA and TA along with their receptors are involved in reproduction, smell perception, metabolism, and homeostasis. Moreover, OA and TA receptors are targets for insecticides and antiparasitic agents, such as the formamidine Amitraz. In the dengue and yellow fever vector, Aedes aegypti, limited research has been reported on their OA or TA receptors. Here, we identify and molecularly characterize the OA and TA receptors in A. aegypti. Bioinformatic tools were used to identify four OA and three TA receptors in the genome of A. aegypti. The seven receptors are expressed in all developmental stages of A. aegypti; however, their highest transcript abundance is observed in the adult. Among several adult A. aegypti tissues examined, including the central nervous system, antennae and rostrum, midgut, Malpighian tubules, ovaries, and testes, the type 2 TA receptor (TAR2) transcript is most abundant in the ovaries and the type 3 TA receptor (TAR3) is enriched in the Malpighian tubules, leading us to propose putative roles for these receptors in reproduction and diuresis, respectively. Furthermore, a blood meal influenced OA and TA receptor transcript expression patterns in adult female tissues at several time points post blood meal, suggesting these receptors may play key physiological roles associated with feeding. To better understand OA and TA signalling in A. aegypti, the transcript expression profiles of key enzymes in their biosynthetic pathway, namely tyrosine decarboxylase (Tdc) and tyramine β-hydroxylase (Tβh), were examined in developmental stages, adult tissues, and brains from blood-fed females. These findings provide information for better understanding the physiological roles of OA, TA, and their receptors in A. aegypti, and additionally, may help in the development of novel strategies for the control of these human disease vectors.

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Section: Discussionsupporting

confidence: 54%

Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles

et al. 2023

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“…Importantly, we found the toxicity activity of bifenazate to this destructive pest, suggesting it could be viewed as an alternative insecticide to control P. xylostella . Previous studies revealed that P. xylostella had evolved high resistance to various classes of insecticides, and sublethal selective pressure could promote the evolution of insecticide resistance in different insects [ 21 , 22 , 23 ]. Additionally, it is known that the identification of sublethal pesticide-responsive genes is required to help us better understand molecular mechanisms of insecticide action and the potential resistance of target insects [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis to Identify Responsive Genes under Sublethal Concentration of Bifenazate in the Diamondback Moth, Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae)

Hou¹,

Zhang²,

Zhu³

et al. 2022

IJMS

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Bifenazate is a novel acaricide that has been widely used to control spider mites. Interestingly, we found bifenazate had a biological activity against the diamondback moth (Plutella xylostella), one of the most economically important pests on crucifer crops around the world. However, the molecular mechanisms underlying the response of P. xylostella to bifenazate treatment are not clear. In this study, we first estimated the LC30 dose of bifenazate for third-instar P. xylostella larvae. Then, in order to identify genes that respond to the treatment of this insecticide, the comparative transcriptome profiles were used to analyze the gene expression changes in P. xylostella larvae after exposure to LC30 of bifenazate. In total, 757 differentially expressed genes (DEGs) between bifenazate-treated and control P. xylostella larvae were identified, in which 526 and 231 genes were up-regulated and down-regulated, respectively. The further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the xenobiotics metabolisms pathway was significantly enriched, with ten detoxifying enzyme genes (four P450s, five glutathione S-transferases (GSTs), and one UDP-Glucuronosyltransferase (UGT)) were up-regulated, and their expression patterns were validated by qRT-PCR as well. Interestingly, the present results showed that 17 cuticular protein (CP) genes were also remarkably up-regulated, including 15 CPR family genes. Additionally, the oxidative phosphorylation pathway was found to be activated with eight mitochondrial genes up-regulated in bifenazate-treated larvae. In contrast, we found some genes that were involved in tyrosine metabolism and purine pathways were down-regulated, indicating these two pathways of bifenazate-exposed larvae were significantly inhibited. In conclusion, the present study would help us to better understand the molecular mechanisms of sublethal doses of bifenazate detoxification and action in P. xylostella.

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“…To meet this opportunity, determining the molecular and pharmacological data of OAR subtypes is an important step. OARs have been characterized in D. melanogaster [ 33 , 40 , 54 ], Bombyx mori [ 55 , 56 ], Periplaneta americana [ 57 , 58 ], A. mellifera [ 41 , 59 , 60 ], N. lugens [ 42 , 49 ], and P. xylostella [ 43 , 46 , 61 , 62 ]. Here, we cloned three OARs from C. chilonis , investigated their expression profiles, and pharmacologically characterized them.…”

Section: Discussionmentioning

confidence: 99%

“…Investigating the properties of OARs can contribute to understand the potential functions of octopaminergic systems in insects. Although the β-adrenergic-like OAR family has been characterized in Drosophila melanogaster [ 40 ], Apis mellifera [ 41 ], Nilaparvata lugens [ 42 ], and Plutella xylostella [ 43 ], the molecular information, expression patterns and pharmacological properties of the β-adrenergic-like OAR family from C. chilonis remain poorly known. The β-adrenergic-like OAR family generally has three subtypes, including Octβ1R, Octβ2R, and Octβ3R.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Pharmacological Characterization of β-Adrenergic-like Octopamine Receptors in the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae)

Zhang

et al. 2022

IJMS

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Octopamine (OA) is structurally and functionally similar to adrenaline/noradrenaline in vertebrates, and OA modulates diverse physiological and behavioral processes in invertebrates. OA exerts its actions by binding to specific octopamine receptors (OARs). Functional and pharmacological characterization of OARs have been investigated in several insects. However, the literature on OARs is scarce for parasitoids. Here we cloned three β-adrenergic-like OARs (CcOctβRs) from Cotesia chilonis. CcOctβRs share high similarity with their own orthologous receptors. The transcript levels of CcOctβRs were varied in different tissues. When heterologously expressed in CHO-K1 cells, CcOctβRs induced cAMP production, and were dose-dependently activated by OA, TA and putative octopaminergic agonists. Their activities were inhibited by potential antagonists and were most efficiently blocked by epinastine. Our study offers important information about the molecular and pharmacological properties of β-adrenergic-like OARs from C. chilonis that will provide the basis to reveal the contribution of individual receptors to the physiological processes and behaviors in parasitoids.

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Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Cited by 9 publications

References 107 publications

Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles

Octopamine and tyramine signalling in Aedes aegypti: Molecular characterization and insight into potential physiological roles

Transcriptome Analysis to Identify Responsive Genes under Sublethal Concentration of Bifenazate in the Diamondback Moth, Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae)

Molecular and Pharmacological Characterization of β-Adrenergic-like Octopamine Receptors in the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae)

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