Pharmacological assessment of zebrafish-based cardiotoxicity models

Maciąg, Monika; Wnorowski, Artur; Mierzejewska, Małgorzata; Płazińska, Anita

doi:10.1016/j.biopha.2022.112695

Cited by 21 publications

(14 citation statements)

References 55 publications

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“…The use of zebrafish larva as a model for cardiotoxic drugs has been demonstrated in several studies ( Maciag et al, 2022 ; Zhu et al, 2014 ). Usually, the cardiotoxic effects are monitored by manually counting the heart rate.…”

Section: Resultsmentioning

confidence: 99%

A new software tool for computer assisted in vivo high-content analysis of transplanted fluorescent cells in intact zebrafish larvae

et al. 2022

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Acute myeloid leukaemia and myelodysplastic syndromes are cancers of the bone marrow with poor prognosis in frail and older patients. To investigate cancer pathophysiology and therapies, confocal imaging of fluorescent cancer cells and their response to treatments in zebrafish larvae yields valuable information. While zebrafish larvae are well suited for confocal imaging, the lack of efficient processing of large datasets remains a severe bottleneck. To alleviate this problem, we present a software tool to segment cells from confocal images and track characteristics such as volume, location in the larva and fluorescent intensity on a single-cell basis. Using this software tool, we were able to characterize the responses of the cancer cell lines Molm-13 and MDS-L to established treatments. By utilizing the computer-assisted processing of confocal images as presented here, more information can be obtained while being less time-consuming and reducing the demand of manual data handling, when compared to a manual approach, thereby accelerating the pursuit of novel anti-cancer treatments. The presented software tool is available as an ImageJ java-plugin at https://zenodo.org/ and the source code at https://github.com/Jfo004/ConfocalCellSegmentation.

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Section: Resultsmentioning

confidence: 99%

A new software tool for computer assisted in vivo high-content analysis of transplanted fluorescent cells in intact zebrafish larvae

et al. 2022

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“…This kind of toxicity is one of the most frequent reasons for drug withdrawals 46 . Harmful reactions identified with the zebrafish model before clinical trials reduce the cost of testing new drugs 45 , 47 . Optical approaches are often applied to evaluating the functioning of the D. rerio cardiovascular system for drug screening 47 – 49 .…”

Section: Discussionmentioning

confidence: 99%

“…Harmful reactions identified with the zebrafish model before clinical trials reduce the cost of testing new drugs 45 , 47 . Optical approaches are often applied to evaluating the functioning of the D. rerio cardiovascular system for drug screening 47 – 49 . Revealed advantages of SWIR imaging can provide an evaluation of the chronic cardiotoxicity in zebrafish with the non-invasive technique.…”

Section: Discussionmentioning

confidence: 99%

Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development

Volkov

Мачихин

Bukova

et al. 2022

Sci Rep

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Optical techniques are utilized for the non-invasive analysis of the zebrafish cardiovascular system at early developmental stages. Being based mainly on conventional optical microscopy components and image sensors, the wavelength range of the collected and analyzed light is not out of the scope of 400–900 nm. In this paper, we compared the non-invasive optical approaches utilizing visible and near infrared range (VISNIR) 400–1000 and the shortwave infrared range (SWIR) 900–1700 nm. The transmittance spectra of zebrafish tissues were measured in these wavelength ranges, then vessel maps, heart rates, and blood flow velocities were calculated from data in VISNIR and SWIR. An increased pigment pattern transparency was registered in SWIR, while the heart and vessel detection quality in this range is not inferior to VISNIR. Obtained results indicate an increased efficiency of SWIR imaging for monitoring heart function and hemodynamic analysis of zebrafish embryos and larvae and suggest a prolonged registration period in this range compared to other optical techniques that are limited by pigment pattern development.

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“…Myocardial cell death induced by ROS is thought to be the molecule mechanism by which Dox damages the myocardium, leading to life-threatening cardiomyopathy [25]. The ROS-staining results (Fig.…”

Section: Categorization Of Differential Metabolitementioning

confidence: 99%

Discovery of key lipids from Panax quinquefolius against doxorubicin-induced cardiotoxicity based on a zebrafish model

Wang

et al. 2023

Preprint

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Objective To discover novel pharmacodynamic substances from Panax quinquefolius against doxorubicin (Dox)-induced cardiotoxicity using a zebrafish model. Methods AB line zebrafish embryos at 30h post-fertilization (hdf) were exposed to Dox (30 µM) for 42h and the heart rate, stroke volume, cardiac area, and fractional shortening of larval zebrafish were used to assess cardiotoxicity. The lipid sample from Panax quinquefolius (PQL) was evaluated the protection of doxorubicin- induced cardiotoxicity compared with the lipids from soybean (SOL) and egg yolk (YOL). The three lipids were analysed using lipidomics techniques based on Q Exactive LC-MS/MS to screen differential lipids. The key lipid was verified the activity against doxorubicin- induced cardiotoxicity using the zebrafish model. Results PQL could significantly alleviate the Dox-induced the decreased heart rate, decreased stroke volume, and decreased fractional shortening (%) on the zebrafish model. 216 differential metabolites were identified, among which the unsaturated fatty acids were the crucial difference components between the three lipid samples. The 18 carbon fatty acids with four carbon–carbon double bonds (FA (18:4)) had been identified and be as a remarkable active compound with protection of Dox-induced cardiotoxicity on the zebrafish model. Conclusion In this research, PQL was discovered firstly to exhibit notable activity against Dox-induced cardiotoxicity in zebrafish, and FA (18:4) was identified as a novel key active component from PQ.

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Pharmacological assessment of zebrafish-based cardiotoxicity models

Cited by 21 publications

References 55 publications

A new software tool for computer assisted in vivo high-content analysis of transplanted fluorescent cells in intact zebrafish larvae

A new software tool for computer assisted in vivo high-content analysis of transplanted fluorescent cells in intact zebrafish larvae

Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development

Discovery of key lipids from Panax quinquefolius against doxorubicin-induced cardiotoxicity based on a zebrafish model

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