Pharmacological Approaches and Regeneration of Bone Defects with Dental Pulp Stem Cells

Adamička, M; Adamičková, A; Danišovič, Ľuboš; Gažová, Andrea; Kyselovič, Ján

doi:10.1155/2021/4593322

Cited by 8 publications

(11 citation statements)

References 52 publications

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“…Aspirin irreversibly acetylates cyclooxygenase, thereby inhibiting COX-1 and COX-2. In recent years, the preventive effect of aspirin on tumors has been widely recognized (Drew et al, 2016;Adamička et al, 2021). In addition, aspirin stimulates the production of endogenous anti-inflammatory and pro-resolution mediators, such as aspirin-triggered resolving (AT-RvDs) and lipoxins (AT-LXs).…”

Section: Influence Of Hydrogel Microtubes On Cell Adhesion Proliferation and Viabilitymentioning

confidence: 99%

Controlled Fabrication of Bioactive Microtubes for Screening Anti-Tongue Squamous Cell Migration Drugs

Tang

Shen

et al. 2022

Front. Chem.

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The treatment of tongue squamous cell carcinoma (TSCC) faces challenges because TSCC has an aggressive biological behavior and manifests usually as widespread metastatic disease. Therefore, it is particularly important to screen out and develop drugs that inhibit tumor invasion and metastasis. Two-dimensional (2D) cell culture has been used as in vitro models to study cellular biological behavior, but growing evidence now shows that the 2D systems can result in cell bioactivities that deviate appreciably the in vivo response. It is urgent to develop a novel 3D cell migration model in vitro to simulate the tumor microenvironment as much as possible and screen out effective anti-migration drugs. Sodium alginate, has a widely used cell encapsulation material, as significant advantages. We have designed a microfluidic device to fabricate a hollow alginate hydrogel microtube model. Based on the difference in liquid flow rate, TSCC cells (Cal27) were able to be evenly distributed in the hollow microtubes, which was confirmed though fluorescence microscope and laser scanning confocal microscope (LSCM). Our microfluidic device was cheap, and commercially available and could be assembled in a modular way, which are composed of a coaxial needle, silicone hose, and syringes. It was proved that the cells grow well in artificial microtubes with extracellular matrix (ECM) proteins by LSCM and flow cytometry. Periodic motility conferred a different motor state to the cells in the microtubes, more closely resembling the environment in vivo. The quantitative analysis of tumor cell migration could be achieved simply by determining the position of the cell in the microtube cross-section. We verified the anti-migration effects of three NSAIDs drugs (aspirin, indomethacin, and nimesulide) with artificial microtubes, obtaining the same results as conventional migration experiments. The results showed that among the three NSAIDs, nimesulide showed great anti-migration potential against TSCC cells. Our method holds great potential for application in the more efficient screening of anti-migration tumor drugs.

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Section: Influence Of Hydrogel Microtubes On Cell Adhesion Proliferation and Viabilitymentioning

confidence: 99%

Controlled Fabrication of Bioactive Microtubes for Screening Anti-Tongue Squamous Cell Migration Drugs

Tang

Shen

et al. 2022

Front. Chem.

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“…The regenerative potential of stem cells isolated from the periodontal ligament (PDLSCs), a highly vascularized connective tissue located between the cementum of the root and alveolar bone socket wall, has been highlighted in several studies [ 24 , 25 , 26 , 27 ]. PDLSCs have been reported to exhibit characteristics similar to BMMSCs, having the potential to differentiate into chondrocytes, osteoblasts, and adipocytes under suitable differentiation conditions [ 24 ]. Their periodontal tissue regeneration potential was confirmed in animal models of periodontal defects, as they were able to form periodontal ligament structures and cementum [ 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…PDLSCs have been reported to exhibit characteristics similar to BMMSCs, having the potential to differentiate into chondrocytes, osteoblasts, and adipocytes under suitable differentiation conditions [ 24 ]. Their periodontal tissue regeneration potential was confirmed in animal models of periodontal defects, as they were able to form periodontal ligament structures and cementum [ 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Botanicals and Oral Stem Cell Mediated Regeneration: A Paradigm Shift from Artificial to Biological Replacement

Ahuja

Tyagi

Kumar

et al. 2022

Cells

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Stem cells are a well-known autologous pluripotent cell source, having excellent potential to develop into specialized cells, such as brain, skin, and bone marrow cells. The oral cavity is reported to be a rich source of multiple types of oral stem cells, including the dental pulp, mucosal soft tissues, periodontal ligament, and apical papilla. Oral stem cells were useful for both the regeneration of soft tissue components in the dental pulp and mineralized structure regeneration, such as bone or dentin, and can be a viable substitute for traditionally used bone marrow stem cells. In recent years, several studies have reported that plant extracts or compounds promoted the proliferation, differentiation, and survival of different oral stem cells. This review is carried out by following the PRISMA guidelines and focusing mainly on the effects of bioactive compounds on oral stem cell-mediated dental, bone, and neural regeneration. It is observed that in recent years studies were mainly focused on the utilization of oral stem cell-mediated regeneration of bone or dental mesenchymal cells, however, the utility of bioactive compounds on oral stem cell-mediated regeneration requires additional assessment beyond in vitro and in vivo studies, and requires more randomized clinical trials and case studies.

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“…Maxillofacial bone defects caused by trauma and tumors are major challenges faced by plastic surgeons, but satisfactory repair materials have not been found yet [1,2]. Titanium alloy, which has been widely used as bone repair material, has obvious demerits.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy

et al. 2021

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3D-printed titanium (Ti) materials have attracted much attention in the field of bone tissue repair. However, the combination strength of traditional alloy materials with bone tissue is lower, and the elastic modulus is higher than that of natural bone tissue, which makes the titanium alloy susceptible to stress shielding phenomena after implantation. Therefore, it is urgent to find better surface modification technology. In this study, the physical and chemical properties, toxicity, and proliferation of adipose stem cells of composite graphene-coated titanium alloy (Gr–Ti) were investigated using 3D-printed titanium alloy as a material model. Physical and chemical property tests confirmed that 3D printing could produce porous titanium alloy materials; the compressive strength and elastic modulus of the titanium alloy scaffolds were 91 ± 3 MPa and 3.1 ± 0.4 GPa, matching the elastic modulus of normal bone tissue. The surface characterization shows that graphene can be coated on titanium alloy by a micro-arc oxidation process, which significantly improves the surface roughness of titanium alloy. The roughness factor (Ra) of the Ti stent was 4.95 ± 1.12 μm, while the Ra of the Gr–Ti stent was 6.37 ± 0.72 μm. After the adipose stem cells were co-cultured with the scaffold for 4 h and 24 h, it was found that the Gr–Ti scaffold could better promote the early cell adhesion. CCK-8 tests showed that the number of ADSCs on the G–Ti scaffold was significantly higher than that on the Ti scaffold (p < 0.01). The relative growth rate (RGR) of ADSCs in Gr–Ti was grade 0–1 (non-toxic). In the in vivo experiment of repairing a critical bone defect of a rabbit mandible, the bone volume fraction in the Gr–Ti group increased to 49.42 ± 3.28%, which was much higher than that in the Ti group (39.76 ± 3.62%) (p < 0.05). In conclusion, the porous graphene–titanium alloy promotes the proliferation and adhesion of adipose stem cells with multidirectional differentiation potential, which has great potential for the application of bone tissue engineering in repairing bone defects in the future.

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Pharmacological Approaches and Regeneration of Bone Defects with Dental Pulp Stem Cells

Cited by 8 publications

References 52 publications

Controlled Fabrication of Bioactive Microtubes for Screening Anti-Tongue Squamous Cell Migration Drugs

Controlled Fabrication of Bioactive Microtubes for Screening Anti-Tongue Squamous Cell Migration Drugs

Botanicals and Oral Stem Cell Mediated Regeneration: A Paradigm Shift from Artificial to Biological Replacement

The Effects of Graphene on the Biocompatibility of a 3D-Printed Porous Titanium Alloy

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