Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1

Liu, Juan‐Ting; Balmori-Cedeño, Jaramar; Misk, Ehab; Lumsden, John S.

doi:10.1007/s11626-020-00490-1

Cited by 6 publications

(19 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…psychrophilum infection (Ryerse et al., 2015, 2016); however, the mechanism responsible for this phenomenon remains unknown. Deoxynivalenol, restricted nutrition and CQ influenced autophagy activity differently in RTgill‐W1 but all reduced the gene copy number and quantity of N‐protein of VHSV IVb in rainbow trout gill epithelial cells (Liu, Balmori‐Cedeno, et al., 2020; Liu, Pham, et al., 2020). The purpose of the present research was to identify the potential role of autophagy modulation in the susceptibility of rainbow trout to experimental infection with F. psychrophilum .…”

Section: Discussionmentioning

confidence: 99%

“…SDS‐PAGE and western blot assay to detect the target protein abundance in the tissues were performed as previously described (Liu, Balmori‐Cedeno, et al., 2020). Membranes with protein less than 25 kDa were incubated with rabbit anti‐LC3B antibody (New England Biolabs), while the membranes with protein more than 25 kDa were incubated with mouse anti‐β‐actin antibody (Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

“…The majority of research on autophagy has taken place in humans, laboratory animals and livestock. Autophagy research in fish is relatively sparse and has focused on a few fish species including zebrafish and rainbow trout (Liu, Balmori‐Cedeno, et al., 2020; Liu, Pham, et al., 2020; Seiliez et al., 2010; Varga et al., 2015; Xia et al., 2019). The research to date has been limited to the influence of autophagy on fish rhabdovirus infection in cell lines.…”

Section: Introductionmentioning

confidence: 99%

“…psychrophilum (Ryerse et al., 2015, 2016). However, reduced nutrition activates autophagy while DON inhibits it in rainbow trout cells in vitro (Liu, Balmori‐Cedeno, et al., 2020). We, therefore, examined autophagy in muscle, liver and spleen of rainbow trout after pretreatment with DON or a restricted ration and then infected similarly treated fish with F .…”

Section: Introductionmentioning

confidence: 99%

“…psychrophilum . In addition, chloroquine (CQ), which blocked autophagy and caused increased expression of autophagy genes in rainbow trout cell lines (Liu, Balmori‐Cedeno, et al., 2020, Balmori‐Cedeno et al 2021), was also used.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Autophagy modulation in rainbow trout Oncorhynchus mykiss L. and resistance to experimental infection with Flavobacterium psychrophilum

Liu

Pham

Lumsden

2022

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Previously, rainbow trout fed deoxynivalenol (DON) or partially fed (pair‐fed) for 4 weeks before and during experimental infection with Flavobacterium psychrophilum had significantly decreased mortality rates. Similar results were obtained in the present study after 12 days, but not after 6 days, feeding 5 ppm DON or pair‐fed before infection. Furthermore, feeding 250 ppm chloroquine (CQ) also reduced mortality (p = .052) compared with controls and may have promise for treatment of some fish disease. Parallel groups of fish were maintained on the respective treatments for 15 days, with an additional group that was fasted, but were not infected to monitor autophagy. Fish that were fasted or fed DON had significantly increased LC3II in the liver and fasted fish had significantly decreased LC3II in muscle compared with controls using western blot. There was no difference in LC3II signal in the spleen of any treatment group. Fish that were fasted or pair‐fed had significant up‐regulation of the Atg genes atg4, atg7, lc3, gabarap and atg12 in muscle using quantitative PCR. Less alteration of Atg expression was seen in liver. Fish treated with CQ had significantly increased expression of atg4, becn1, lc3 and atg12 in the liver. Fish fed DON for 15 days had few alterations of Atg genes in either the liver or muscle. It is still not clear if autophagy is responsible for the resistance of rainbow trout fed DON, CQ or pair‐fed before F. psychrophilum infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Autophagy modulation in rainbow trout Oncorhynchus mykiss L. and resistance to experimental infection with Flavobacterium psychrophilum

Liu

Pham

Lumsden

2022

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

Transcriptomic Points of Departure Calculated from Rainbow Trout Gill, Liver, and Gut Cell Lines Exposed to Methylmercury and Fluoxetine

Mittal

Ewald

Basu

2022

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Ethical and resource limitation concerns are pushing chemicals management to develop alternatives to animal testing strategies. The objective of our study was to determine whether transcriptomic point of departure (tPOD) values could be derived from studies that followed Organisation for Economic Co‐operation and Development (OECD) Test No. 249 (rainbow trout gill cell line), as well as from studies on trout liver and gut cells. Gill, liver, and gut cell lines were exposed to methylmercury and fluoxetine. Concentrations causing 50% cytotoxicity (LC50) were derived, the whole transcriptome was sequenced, and gene tPOD and pathway benchmark dose (BMD) values were derived from transcriptomic dose–response analysis. Differences in LC50 and transcriptomic responses across the cell lines were noted. For methylmercury, the tPODmode values were 14.5, 20.5, and 17.8 ppb for the gill, liver, and gut cells, respectively. The most sensitive pathway (pathway BMDs in parentheses) was ferroptosis in the gill (3.1 ppb) and liver (3.5 ppb), and glutathione metabolism in the gut (6.6 ppb). For fluoxetine, the tPODmode values were 109.4, 108.4, and 97.4 ppb for the gill, liver, and gut cells, respectively. The most sensitive pathway was neurotrophin signaling in the gill (147 ppb) and dopaminergic signaling in the gut (86.3 ppb). For both chemicals, the gene tPOD and pathway BMD values were lower than cytotoxic concentrations in vitro, and within 10‐fold below the in vivo LC50s. By bringing together transcriptomics and dose–response analysis with an OECD test method in three cell lines, the results help to establish an in vitro method yielding tPOD values that are hypothesized to be protective of in vivo concentrations associated with adverse outcomes, and also give insights into mechanisms of action. Environ Toxicol Chem 2022;41:1982–1992. © 2022 SETAC

show abstract

miR-199-5p mediates the regulation of autophagy by targeting mTOR signaling and involvement in ammonia detoxification under ammonia stress in yellow catfish (Pelteobagrus fulvidraco)

Wang,

Li,

Zhang

et al. 2024

Aquaculture

View full text Add to dashboard Cite

Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1

Cited by 6 publications

References 65 publications

Autophagy modulation in rainbow trout Oncorhynchus mykiss L. and resistance to experimental infection with Flavobacterium psychrophilum

Autophagy modulation in rainbow trout Oncorhynchus mykiss L. and resistance to experimental infection with Flavobacterium psychrophilum

Transcriptomic Points of Departure Calculated from Rainbow Trout Gill, Liver, and Gut Cell Lines Exposed to Methylmercury and Fluoxetine

miR-199-5p mediates the regulation of autophagy by targeting mTOR signaling and involvement in ammonia detoxification under ammonia stress in yellow catfish (Pelteobagrus fulvidraco)

Contact Info

Product

Resources

About