Pharmacological and Nutraceutical Activation of Rejuvenation, Geroprotection and Cytoprotection: Proofs of Concept

Lizard, Gérard; Hammami, Mohamed; Poli, Giuseppe

doi:10.3390/cells11233786

Cited by 1 publication

(1 citation statement)

References 38 publications

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“…In developing a basic approach and defining anti-aging and rejuvenation activity, it is possible to start from the human as a whole, organs and functional sub-systems, or from the cell level. Indeed, a broad literature is available on the cell-level anti-aging and rejuvenation strategies, methods and studies ( [31][32][33][34][35][36][37][38] can be suggested as more general publications and reviews relevant to the discussed topic). Large number of researchers directly correlate human aging to the processes at the cell level [39][40][41][42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Novel Strategy in Searching for Natural Compounds with Anti-Aging and Rejuvenating Potential

Koptyug

Sukhovei

Kostolomova

et al. 2023

IJMS

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We suggest a novel approach for searching natural compounds with anti-aging and rejuvenation potential using cell cultures, with a high potential for the further in vivo applications. The present paper discusses ways of defining age for cell populations with large numbers of cells and suggests a method of assessing how young or old a cell population is based on a cell age profile approach. This approach uses experimental distributions of the cells over the cell cycle stages, acquired using flow cytometry. This paper discusses how such a profile should evolve under homeostatic maintenance of cell numbers in the proliferation niches. We describe promising results from experiments on a commercial substance claiming rejuvenating and anti-aging activity acting upon the cultures of human mononuclear cells and dermal fibroblasts. The chosen substance promotes a shift towards larger proportion of cells in synthesis and proliferation stages, and increases cell culture longevity. Further, we describe promising in vivo testing results of a selected food supplement. Based on the described concept of cell age profile and available test results, a strategy to search for natural compounds with regenerative, anti-aging and rejuvenation potential is suggested and proposed for wider and thorough testing. Proposed methodology of age assessment is rather generic and can be used for quantitative assessment of the anti-aging and rejuvenation potential of different interventions. Further research aimed at the tests of the suggested strategy using more substances and different interventions, and the thorough studies of molecular mechanisms related to the action of the substance used for testing the suggested search methodology, are needed.

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