Pharmacological analysis of vasoconstrictor responses of isolated and perfused human umbilical arteries

Yoshikawa, Fumihiko; Chiba, Shigetoshi

doi:10.1007/bf02125097

Cited by 10 publications

(12 citation statements)

References 22 publications

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“…These results are in agreement with those previously published by Somlyo et al [21] and more recently by Yoshikawa and Chiba [22].…”

Section: Discussionsupporting

confidence: 83%

Effects of Magnesium on Human Umbilical Arteries: Role of Intracellular Calcium Stores

Tiritilli

1998

Pharmacology

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The purpose of this investigation on human umbilical arteries was: (1) to analyze the effects of magnesium (Mg²⁺) in the prevention of contractions induced by 5-hydroxytryptamine (5-HT), KCl, Bay K 8644 and BaCl₂; (2) to determine whether the presence of Mg²⁺ could modulate the response to caffeine in Ca²⁺-free medium at 25 and 37°C, and (3) to examine the influence of Mg²⁺ and temperature in the release of intracellular calcium induced by 5-HT in Ca²⁺-free medium. Human umbilical arteries were dissected and mounted in organ baths for isometric measurement of force. Our results showed: (1) Mg²⁺ is a potent inhibitor of smooth muscle contraction of human umbilical arteries caused by 5-HT, KCl, Bay K 8644 and BaCl₂, and that this effect might be due to the inhibition of the transmembrane calcium influx. (2) The relaxant effect of Mg²⁺ did not seem to be mediated by a blockade of the release of calcium from an intracellular store. (3) Moreover, our results suggest an effect of temperature independent from the action of Mg²⁺ on 5-HT-induced contraction.

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“…These results are in agreement with those previously published by Somlyo et al [21] and more recently by Yoshikawa and Chiba [22].…”

Section: Discussionsupporting

confidence: 83%

Effects of Magnesium on Human Umbilical Arteries: Role of Intracellular Calcium Stores

Tiritilli

1998

Pharmacology

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“…In the present study, ACh‐induced vasoconstriction of control HUV rings was not influenced by endothelium. Similar results were reported in human umbilical artery ( Yoshikawa & Chiba, 1991 ). Our results are in accordance with previous studies employing a bioassay cascade perfusion technique, which showed that ACh did not release endothelium‐derived relaxing factor in HUV ( Van de Voorde et al ., 1987 ; Chaudhuri et al ., 1991 ).…”

Section: Discussionmentioning

confidence: 99%

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pujol-Lereis

Hita

Gobbi

et al. 2006

British J Pharmacology

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1 The present study attempted to pharmacologically characterize the muscarinic receptor subtypes mediating contraction of human umbilical vein (HUV). 2 HUV rings were mounted in organ baths and concentration-response curves were constructed for acetylcholine (ACh) (pEC 50 : 6.1670.04; maximum response 80.0071.98% of the responses induced by serotonin 10 mM). The absence of endothelium did not modify the contractile responses of ACh in this tissue. 3 The role of cholinesterases was evaluated: neither neostigmine (acetylcholinesterase inhibitor) nor iso-OMPA (butyrylcholinesterase inhibitor) modified ACh responses. When both enzymes were simultaneously inhibited, a significantly but little potentiation was observed (control: pEC 50 6.3370.03; double inhibition: pEC 50 6.5770.05). 4 Atropine, nonselective muscarinic receptors antagonist, inhibited ACh-induced contraction (pK B 9.67). The muscarinic receptors antagonists pirenzepine (M 1 ), methoctramine (M 2 ) and pFHHSiD (M 3 ) also antagonized responses to ACh. The affinity values estimated for these antagonists against responses evoked by ACh were 7.58, 6.78 and 7.94, respectively. On the other hand, PD 102807 (M 4 selective muscarinic receptors antagonist) was ineffective against ACh-induced contraction. 5 In presence of a blocking concentration of pirenzepine, pFHHSiFD produced an additional antagonism activity on ACh-induced responses. 6 The M 1 muscarinic receptors agonist McN-A-343 produced similar maximum but less potent responses than ACh in HUV. The calculated pA 2 for pirenzepine against McN-A-343 induced responses was 8.54. 7 In conclusion, the data obtained in this study demonstrate the role of M 1 muscarinic receptor subtypes and suggest the involvement of M 3 muscarinic receptor subtypes in ACh-induced vasoconstriction in HUV rings. In addition, the vasomotor activity evoked by ACh does not seem to be modulated by endothelial factors, and their enzymatic degradation appears to have little functional relevance in this tissue.

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“…Catecholamines exert different effects on arteries and veins due to different artery–vein differences in α‐adrenoceptor expression . Previous data has shown that human umbilical arteries also exhibit different pharmacological responses from those of vessels of other organs . The contractile adrenoreceptors in the human umbilical artery smooth musculature comprise α‐adrenoceptors (α1‐ and α2‐subtypes) and D1 receptors .…”

Section: Introductionmentioning

confidence: 99%

“…3,4 Previous data has shown that human umbilical arteries also exhibit different pharmacological responses from those of vessels of other organs. 5 The contractile adrenoreceptors in the human umbilical artery smooth musculature comprise α-adrenoceptors (α1-and α2-subtypes) and D1 receptors. 6,7 On the other hand, in umbilical veins, both functional βand α2-adrenoreceptors are absent, and the α1-receptors are responsible for the vasoconstrictor effects of catecholamines in the human umbilical vein.…”

Section: Introductionmentioning

confidence: 99%

Effects of propofol and sevoflurane on isolated human umbilical arteries pre‐contracted with dopamine, adrenaline and noradrenaline

Gunduz

Arun

Bagci

et al. 2014

J of Obstet and Gynaecol

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Dopamine, adrenaline and noradrenaline elicit contractions in human umbilical arteries, and noradrenaline causes the highest contraction. Both propofol and sevoflurane inhibit these contractions in a dose-dependent manner. Propofol caused greater relaxation in the contractions elicited by dopamine and adrenaline while sevoflurane caused greater relaxation in the contraction elicited by noradrenaline.

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Pharmacological analysis of vasoconstrictor responses of isolated and perfused human umbilical arteries

Cited by 10 publications

References 22 publications

Effects of Magnesium on Human Umbilical Arteries: Role of Intracellular Calcium Stores

Effects of Magnesium on Human Umbilical Arteries: Role of Intracellular Calcium Stores

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Effects of propofol and sevoflurane on isolated human umbilical arteries pre‐contracted with dopamine, adrenaline and noradrenaline

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