Pharmacologic Alternatives to Riboflavin Photochemical Corneal Cross-Linking: A Comparison Study of Cell Toxicity Thresholds

Abstract

There are significant differences in cell toxicity among potential topical cross-linking compounds. The balance between cross-linking of tissue and cell toxicity should be borne in mind as compounds and strategies to improve mechanical tissue properties through therapeutic tissue cross-linking continue to develop.

Cosmetic Preservatives as Therapeutic Corneal and Scleral Tissue Cross-Linking Agents

“…Formaldehyde-releasing agent cell cytotoxicity assays were performed as we have previously described. 33 Briefly, healthy human skin fibroblasts (HSFs) from American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in dermal cell basal media (ATCC) using a serum-free fibroblast growth kit provided by the company (ascorbic acid, EGF/TGF-b1, glutamine, hydrocortisone, insulin, and fibroblast growth supplements). Cells were grown in 5% CO 2 and 95% ambient air at 378C until confluent.…”

Global Consensus on Keratoconus and Ectatic Diseases

“…The majority of this research focuses on new antiinflammatory molecules or innovative technologies to induce transepithelial CXL. 50,51 It is possible that in the future, the researchers will find a topical medication that could directly influence the progression of keratoconus and other corneal ectasias.…”

Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

“…This result is in accordance with previously published data on the toxicity of this crosslinking agent. 29 It is worth mentioning that this toxic effect was clearly concentration-dependent. Zhong et al reported the use of vapors of GA to efficiently crosslink polysaccharide structures without toxic effect associated.…”