Pharmacokinetics of Tetracycline and Tetracycline Loaded Nanoemulsion Formula in Rabbits

Amer, Aziza M.; Badawy, Shymaa A. El; Saber, Mohamed; Ahmed-Farid, Omar A.; Abd-Elsalam, Wessam H.; Amer, M. M.

doi:10.17582/journal.aavs/2020/8.2.130.139

Cited by 3 publications

(8 citation statements)

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“…Also, higher k12 0.765 ± 0.361 1/h and slow k21 1.431 ± 0.75 1/h were recorded in TC-nm as compared with those ofTC-hcl k12 0.683 ± 0.511 1/hand k21 2.1 ± 1.8 1/h., respectively. These results are in accordance with those reported previously by many animal species inrats [48], female rats and male guinea-pigs [52], adult white Californian rabbits [30,54] and dogs [49]. These findings represented by higher and prolonged tetracycline plasma concentration after TC-nmadministration than TC-hcl powder [30].…”

Section: Table1 Plasma Concentration Of Tc-hcland Tc-nm After Oral Osupporting

confidence: 93%

“…The mean plasma tetracycline concentrationtime relationship following a single oral administration of 50 mg/kg of BW (Table 1) Tc-hcl concentration in plasma started to be detected at 0.25 h, reach the maximum at 0.5 h followed by decline at 12 hours as 4.26 ± 1.458μg/ml ,7.60±1.102μg/ ml and 0.03±0.005μg/ml, respectively. While, TC-nm was determined at 0.25 h and reach the maximum at 1 h and decline to the minimum value at 12 h as 3.77 ± 0.923μg/ml, 7.41±2.184μg/ ml and 0.08± 0.008μg/ml, respectively.Similar results were detected in rat [48], in dogs [49], in man [50], in rabbit [30]. The drug had a rapid distribution phase [31,51].The non-detected concentration at 24 was reported in dog [27].…”

Section: Pharmacokineticssupporting

confidence: 77%

“…The drug had a rapid distribution phase [31,51].The non-detected concentration at 24 was reported in dog [27]. μNanoemulsion showed higher concentrations persisted higher than MIC for longer time (more PHARMACOLOGICAL STUDIES ON TETRACYCLINE AND TETRACYCLINE … than 10 hours) than that for powder form 6 hours ( Table 1, Fig 1) [30].…”

Section: Pharmacokineticsmentioning

confidence: 95%

“…Animals of group 1 given TC-nm and animals of group 2 given Tc-hcl. Single dose of 50 mg/kg body weight (BW)from each preparation will be given for each rabbit using oral and intravenous (i.v) route, with 14 day interval to insure complete drug clearance from rabbits body [30,32,33]. Blood samples were collected at different time intervals at 0.083 (5 min), 0.15 (0.15 min), 0.5 (30 min), 1, 2, 4, 6, 8, 10, 12 and 24 hours after each dose administration.Individual non-coagulated blood samples were collected from ear vein through i.v catheter for separation of plasma [34,35].The collected plasma was stored at -80 ºC till determination of tetracycline using microbiological technique.…”

Section: Groups and Administrationmentioning

confidence: 99%

“…A significant higher AUC0inf. ( [29,30], in sheep [23]. While tetracyclinewasdetected for 30 hours after oral dose of 40 mg/kg in pigs [55].…”

Section: Table1 Plasma Concentration Of Tc-hcland Tc-nm After Oral Omentioning

confidence: 99%

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Pharmacological studies on tetracycline and tetracycline nanoemulsion formulas. Mohamed S. Saber1, Aziza M. Amer2, Shymaa A. ElBadawy2, Mohamed M. Amer3, 1. PhD student in Pharmacol. , Vet. Service Division, Ministry of Defense, Egypt.. 2. 2. Dept. of Pharmacol., Facult. Vet. Med., Cairo University, Giza, Egypt. 3. 3. Dept. of Poult. Dis., Facult. Vet. Med., Cairo University, P.O. 12211, Giza, Egypt.

et al. 2019

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T HE study was done to compare the pharmacokinetic and pharmacodynamics of 50 mg/ kg b.wttetracycline hydrochloride (TC-hcl) and tetracycline nanoemulsion (TC-nm) formulasin rabbits and detection of their effect on stander and field bacterial strains. After oral TC concentration in plasma started to be detected at 0.25 h, reach the maximum at (0.5 h Tc-hcl) and 1 h.(TC-nm) and decline at 12 hours. Following a single i.v administration a volume of distribution V2 (0.292±0.111 L/kg) in TC-nm than for TC-hcl (0.216 ± 0.183L/kg) and was slowly cleared (0.393±0.183 L.h/kg) in Tc-nm than in TC-hcl (0.415±0.311 L.h/kg). After oral administration a rapidly absorbed with significant slowly absorption half-life t1/2 alpha (0.550±0.090 h and 0.176±0.058 h.) and elimination half-life t1/2 beta (4.215±1.661 h. and 1.58±1.447 h.) with higher calculated Cmax of (4.215±1.661 μg/ml and 1.58±1.447 μg/ml) achieved at prolonged calculated tmax (0.759±0.149h. and 0.356±0.305 h.) in TC-nm than in TC-hcl, respectively. The value of TC-hcl and TC-nm MIC was the same for Staph. Aureus 6538, Staph. Epidermidis12228 ,E.coli 8739 were 0.14, 0.8 and 0.12 µg , respectively, and interpreted as sensitive. Field sensitive Corynebacterium, E.coli , S. typhimurium, S. enteriditisand Staph.leutus isolates MIC value was 1.4, 8, 2, 1.6 and 2 µg for , respectively. Tetracycline resistant2Staph.scuiri (18 and 16µg) and 2 Staph. xylosis (18 and 6µg).

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Section: Table1 Plasma Concentration Of Tc-hcland Tc-nm After Oral Osupporting

confidence: 93%

Section: Pharmacokineticssupporting

confidence: 77%

Section: Pharmacokineticsmentioning

confidence: 95%

Section: Groups and Administrationmentioning

confidence: 99%

“…A significant higher AUC0inf. ( [29,30], in sheep [23]. While tetracyclinewasdetected for 30 hours after oral dose of 40 mg/kg in pigs [55].…”

Section: Table1 Plasma Concentration Of Tc-hcland Tc-nm After Oral Omentioning

confidence: 99%

See 3 more Smart Citations

Pharmacological studies on tetracycline and tetracycline nanoemulsion formulas. Mohamed S. Saber1, Aziza M. Amer2, Shymaa A. ElBadawy2, Mohamed M. Amer3, 1. PhD student in Pharmacol. , Vet. Service Division, Ministry of Defense, Egypt.. 2. 2. Dept. of Pharmacol., Facult. Vet. Med., Cairo University, Giza, Egypt. 3. 3. Dept. of Poult. Dis., Facult. Vet. Med., Cairo University, P.O. 12211, Giza, Egypt.

et al. 2019

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Nano Spray-Dried Drugs for Oral Administration: A Review

Öztürk

Arpagaus

2021

ASSAY and Drug Development Technologies

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Spray drying is an important technology that is fast, simple, reproducible, and scalable. It has a wide application range, that is, in food, chemicals, and encapsulation of pharmaceuticals. The technology can be divided into conventional spray drying and nano spray drying. The key advantage of nano spray drying is the production of drug-loaded nanosized particles for various drug delivery applications. The recent developments in nano spray dryer technology and the market launch of the Nano Spray Dryer B-90 by Bu ¨chi Labortechnik AG in 2009 enabled the production of submicron spray-dried particles. This review focuses on nanosized drug delivery systems intended for oral administration produced by nano spray drying. First, the nano spray drying concept, the basic technologies implemented in the equipment, and the effects of the various process parameters on the final dry submicron powder properties are presented. Then, the topics of new formulation strategies of oral drugs are highlighted with examples that have entered the research literature in recent years. Next, the subjects of direct conversion of poorly watersoluble drugs, encapsulation of drugs, and drying of preformed nanoparticles are considered. Finally, topics such as morphology, particle size, size distribution, surface analysis, bioavailability, drug release, release kinetics, and solid-state characterization (by differential scanning calorimetry, X-ray diffraction, Fourier transform infrared spectroscopy, nuclear magnetic resonance) of oral drug delivery systems produced by nano spray drying are discussed. The review attempts to provide a comprehensive knowledge base with current literature and foresight to researchers working in the field of pharmaceutical technology and nanotechnology and especially in the field of nano spray drying.

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Protective potential of frankincense essential oil and its loaded solid lipid nanoparticles against UVB-induced photodamage in rats via MAPK and PI3K/AKT signaling pathways; A promising anti-aging therapy

Kotb,

El-Shiekh,

Abd-Elsalam

et al. 2023

PLoS ONE

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Frankincense oil has gained increased popularity in skin care, yet its anti-aging effect remains unclear. The current study aimed to investigate the anti-photoaging effect of frankincense (Boswellia papyrifera (Del.) Hochst., Family Burseraceae) essential oil in an in vivo model. The oil was initially extracted by two methods: hydro-distillation (HD) and microwave-assisted hydro-distillation (MAHD). GC/MS analysis revealed the dominance of n-octyl acetate, along with other marker compounds of B. papyrifera including octanol and diterpene components (verticilla 4(20) 7, 11-triene and incensole acetate). Thereafter, preliminary investigation of the anti-collagenase and anti-elastase activities of the extracted oils revealed the superior anti-aging effect of HD-extracted oil (FO), comparable to epigallocatechin gallate. FO was subsequently formulated into solid lipid nanoparticles (FO-SLNs) via high shear homogenization to improve its solubility and skin penetration characteristics prior to in vivo testing. The optimimal formulation prepared with 0.5% FO, and 4% Tween® 80, demonstrated nanosized spherical particles with high entrapment efficiency percentage and sustained release for 8 hours. The anti-photoaging effect of FO and FO-SLNs was then evaluated in UVB-irradiated hairless rats, compared to Vitamin A palmitate as a positive standard. FO and FO-SLNs restored the antioxidant capacity (SOD and CAT) and prohibited inflammatory markers (IL6, NFκB p65) in UVB-irradiated rats via downregulation of MAPK (pERK, pJNK, and pp38) and PI3K/AKT signaling pathways, alongside upregulating TGF-β expression. Subsequently, our treatments induced Procollagen I synthesis and downregulation of MMPs (MMP1, MMP9), where FO-SLNs exhibited superior anti-photoaging effect, compared to FO and Vitamin A, highlighting the use of SLNs as a promising nanocarrier for FO. In particular, FO-SLNs revealed normal epidermal and dermal histological structures, protected against UVβ-induced epidermal thickness and dermal collagen degradation. Our results indicated the potential use of FO-SLNs as a promising topical anti-aging therapy.

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Pharmacokinetics of Tetracycline and Tetracycline Loaded Nanoemulsion Formula in Rabbits

Cited by 3 publications

References 1 publication

Nano Spray-Dried Drugs for Oral Administration: A Review

Protective potential of frankincense essential oil and its loaded solid lipid nanoparticles against UVB-induced photodamage in rats via MAPK and PI3K/AKT signaling pathways; A promising anti-aging therapy

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