Pharmacokinetics of Superoxide Dismutase in Rats After Oral Administration

Regnault, C.; Soursac, Mireille; Roch-Arveiller, M; Postaire, E.; Hazebroucq, G.

doi:10.1002/(sici)1099-081x(199603)17:2<165::aid-bdd945>3.0.co;2-n

Cited by 38 publications

(14 citation statements)

References 2 publications

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“…This finding raises the question of the putative mechanism of action of Glisodin w when administered by oral route. Indeed, while vegetal SOD, as any orally administered protein, is usually deteriorated by gastric digestion and thus ineffective, [4] combining SOD with wheat gliadin renders it biologically effective. In addition, the vehicle gliadin per se might also have an additional effect at the gut wall level.…”

Section: Discussionsupporting

confidence: 45%

“…flavonoids) are effective when administered orally, until recently efficient oral delivery of antioxidant enzymes has been limited by the gastro-intestinal digestive processes. [4] A new nutritional formula (Glisodin w ) containing a plant (Cucumis melo LC) superoxide dismutase (SOD) extract chemically combined with a gliadin biopolymer system allows overcoming this limitation. [5,6] In a prospective, double-blind randomised placebocontrolled study with healthy volunteers, we therefore tested the hypothesis that an orally effective mixture of the antioxidant enzyme SOD with gliadin allows preventing DNA strand-breaks affiliated with exposure to HBO.…”

Section: Introductionmentioning

confidence: 98%

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Influence of an Orally Effective SOD on Hyperbaric Oxygen-related Cell Damage

Muth

Glenz

Klaus

et al. 2004

Free Radical Research

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In a prospective, double-blind, randomised placebocontrolled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress.Twenty healthy volunteers were exposed to 100% oxygen breathing at 2.5 ATA for a total of 60 min. DNA strand breaks (tail moments) were determined using the alkaline version of the comet assay. Whole blood concentrations of reduced (GSH) and oxidised (GSSG) glutathione and F 2 -isoprostanes, SOD, glutathione peroxidase (GPx) and catalase (Cat) activities and red cell malondialdehyde (MDA) content were determined.After HBO exposure the tail moment ðp ¼ 0:03Þ and isoprostane levels ðp ¼ 0:049Þ were significantly lower in the group that received the vegetal formulation. Neither SOD and Cat nor GSH and GSSG were significantly affected by this preparation or HBO exposure. By contrast, blood GPx activity, which tended to be lower in the SODgroup already before the HBO exposure ðp ¼ 0:076Þ; was significantly lower afterwards ðp ¼ 0:045Þ:We conclude that an orally effective SOD-wheat gliadin mixture is able to protect against DNA damage, which coincided with reduced blood isoprostane levels, and may therefore be used as an antioxidant.

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Section: Discussionsupporting

confidence: 45%

Section: Introductionmentioning

confidence: 98%

Influence of an Orally Effective SOD on Hyperbaric Oxygen-related Cell Damage

Muth

Glenz

Klaus

et al. 2004

Free Radical Research

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“…[15] However, it is quickly eliminated from the bloodstream [16] and rapidly degraded in the gastrointestinal tract, thereby compromising its delivery. [17] Attempts have been made to improve its delivery by protecting the protein via covalent modification with poly(ethylene glycol) (PEG) or phosphatidylcholine [18,19] and by encapsulation into liposomes. [20,21] However, these approaches did not solve the low bioavailability of SOD because an excessive covalent modification with PEG compromises SOD activity, [22] and encapsulation of SOD into liposomes has serious drawbacks, such as mechanical instability, interaction with high-density lipoproteins, and a short circulation lifetime.…”

Section: Introductionmentioning

confidence: 99%

SOD Antioxidant Nanoreactors: Influence of Block Copolymer Composition on the Nanoreactor Efficiency

Onaca

Hughes

Balasubramanian

et al. 2010

Macromolecular Bioscience

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The bioavailability limitations of proteins make them difficult to be directly delivered, particularly in diseases caused by insufficient amounts or inactive variants of those proteins. Nanoreactors represent a new promising approach to overcome these limitations because they serve both to protect the protein in their aqueous interior, and simultaneously to allow the protein to act in situ. Here we examine an antioxidant nanoreactor based on SOD encapsulated in amphiphilic block copolymer nanovesicles, and analyze its behavior as a function of the copolymer composition. The membrane of the triblock copolymer nanovesicles plays a double role, both to shield the sensitive protein and selectively to let superoxide and dioxygen penetrate to its inner space. The encapsulation efficiency for different triblock copolymer vesicles was quantified by fluorescence correlation spectroscopy using a fluorescently labeled SOD. Pulse radiolysis experiments and an enzymatic assay were used to compare the permeability of the wall-forming membranes towards superoxide anions. While the encapsulation efficiency mainly depends on the vesicle dimensions, the membrane permeability is mainly affected by the length of the hydrophobic PDMS middle blocks of our polymers. For polymers with very long PDMS chains superoxide anion transport across the membranes was too slow to be detected by our experiments.

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“…SOD) as nutraceutical products but the results were disappointing because of the poor bioavailability of these non-protected molecules (Regnault et al, 1996;Giri and Misra, 1984;Zidenberg-Cherr et al, 1983). In the present study we demonstrated that the SOD activity presents in a Cucumis melo LC.…”

Section: Introductionmentioning

confidence: 99%