Pharmacokinetics of oxolinic acid in sea‐bass, Dicentrarchus labrax (L., 1758), after a single rapid intravascular injection

Abstract

The pharmacokinetics of oxolinic acid was studied in sea-bass (Dicentrarchus labrax). The fish were kept in seawater at 15.2 degrees C with a 12 h/12 h photoperiod. Oxolinic acid was injected in the caudal vein of anaesthetized sea-bass in a single rapid intravascular administration at a dose of 10 mg/kg of body weight. Plasma concentrations of oxolinic acid were determined using two analytical methods, a classic plate diffusion bioassay using Escherichia coli and a high performance liquid chromatography (HPLC) using solid phase extraction with an internal standard and a U.V. detection. The mean recoveries were 99.6% and 110.8% and determination limits were 0.04 microg/mL and 0.02 microg/mL, for the bioassay and the HPLC respectively. Compared to other fish species, the oxolinic acid was rapidly (absorption half life, t(a1/2) = 0.69 h) distributed to body tissues outside the blood volume (volume of central compartment, Vc = 0.4 L/kg) and presented a large volume of distribution (Vdss = 2.55 L/kg). Considering its disappearance from the central compartment (rate constant: central-eliminated, k10 = 0.16 h[-1]) and its total body clearance (Cl[t] = 0.066 L/kg x h), the elimination phase of the oxolinic acid in sea-bass was shorter than in trout kept in freshwater, and longer than in salmon in seawater. Consequently, the area under the concentration-time curve (AUC = 157 microg x h/mL) and the mean residence time (MRT = 42 h) were relatively low and short, respectively.

Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L.

“…1993; Kleinow et al . 1994; Martinsen & Horsberg 1995; Poher et al . 1997; Samuelsen & Ervik 1999; Samuelsen et al .…”

“…1994). Longer t 1/2α of OA have been calculated in sea bass (0.69 h) (Poher et al . 1997), in Atlantic halibut (7 h) (Samuelsen & Ervik 1999) and in Atlantic salmon (0.7–4.7 h) (Rogstad et al .…”

Antibacterial Agents in Mediterranean Finfish Farming: A Synopsis of Drug Pharmacokinetics in Important Euryhaline Fish Species and Possible Environmental Implications

“…Although the penetration of intravascularyinjected OA in the tissue compartment of euryhaline fish is moderate (V d(ss) =2.1-2.6 L kg )1 ) (Poher et al, 1997;Rigos et al, 2002cRigos et al, , 2004d it is higher than that of FLU (0.6-1.5 L kg )1 ) (Rigos et al, 2002e, 2003c. A study of the tissue distribution of OA following multiple dosing, revealed that liver, bile and skin act as reservoirs of the drug treatment in both gilthead and sharpsnout sea bream with rapid depletion of the drug from all tissues to ''consumer safe levels'' (MRL = 100 ng g )1 ; EMEA, 2003) 24 h after completion of treatment at 19°C (Rigos et al, 2003b).…”

Comparative pharmacokinetics and bioavailability of oxytetracycline in giant tiger prawn

“…These parameters are related to the duration and intensity of drug action and can be used to obtain a meaningful measure of the drug's usefulness, possible toxicity, residues etc. In aquatic animals, several researchers have investigated the pharmacokinetics of antibiotics and antibacterial agents 1–5 . However, few papers have been concerned with pharmacokinetics of drugs in shrimp 6 and lobster 7,8 …”