Pharmacokinetics of eCG and induction of fertile estrus in bitches using eCG followed by hCG

Mitacek, María Carla García; Gimenez, Fernanda; Bonaura, María Candela; Dorna, I. Videla; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra

doi:10.1016/j.theriogenology.2012.04.012

Cited by 10 publications

(31 citation statements)

References 34 publications

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“…These results are in agreement with previous findings from Stornelli et al. (), who observed that 50 UI of eCG induced follicular growth in all animals studied.…”

Section: Discussionsupporting

confidence: 94%

“…The results of the present study are in agreement with those of Stornelli et al. () and England et al. (), who used a protocol combining eCG followed hCG for oestrus induction in the bitch obtaining an increase of progesterone in 100% of treatment bitches (England et al.…”

Section: Discussionsupporting

confidence: 93%

“…Briefly, each study animal was observed for SB and CSE and scored from 1 to 3 (Table ; Stornelli et al. ). Swabs were introduced 6–10 cm into the vagina, and quickly and gently rotated against the floor and lateral walls of the vagina.…”

Section: Methodsmentioning

confidence: 99%

“…According to the percentage and type of cells present, samples were scored from 1 to 5 (Table ; Stornelli et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…; Stornelli et al. ). A protocol using 50 IU/kg of eCG combined 7 days later with 500 IU of hCG induced normal and fertile oestrus at 164 days post‐oestrus with an 80% pregnancy rate (Stornelli et al.…”

Section: Introductionmentioning

confidence: 96%

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Prolactin, Androstenedione and IGF1 Serum Concentrations During Induced Follicular Growth by eCG Administration in the Bitch

Stornelli

Mitacek

Praderio

et al. 2015

Reprod Domestic Animals

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The oestrus cycle in the domestic bitch, a monoestrous species, differs considerably from that of other veterinary domestic animals species. In the bitch the combined use of eCG and hCG is effective to induce oestrus predictably and safely (Stornelli et al., Theriogenology, 78, 2012 and 1056). Although several studies were done to describe the hormonal changes during the canine oestrus cycle, to our knowledge none was done to describe the hormonal changes during induced follicular growth after the administration of eCG. The aim of this work was to study prolactin (PRL), insulin-like growth factor (IGF1) and androstenedione (ANDR) serum concentrations during follicular growth induced by a single dose of eCG administered to late anoestrous bitches. PRL and ANDR concentrations were lower before than after eCG TRT (before eCG vs pro-oestrus, oestrus and dioestrus; 4.3 ± 1.8 ng/ml vs 6.5 ± 1.6 ng/ml, p < 0.05; 0.08 ± 0.2 ng/ml vs 0.42 ± 0.16 ng/ml, p < 0.05). Conversely, IGF1 concentrations were similar before and after eCG TRT (286.0 ng/ml ±32.2, p > 0.53). Additionally, PRL concentrations were similar before oestrus compared to during oestrus and dioestrus (6.9 ± 1.7 ng/ml, p > 0.19). Furthermore, IGF1 concentrations were higher before and during oestrus compared to first day of dioestrus (286.1 ± 29.8vs 200.4 ± 29.2 ng/ml, p < 0.01). On the contrary, ANDR concentrations were lower before and during oestrus compared to first day of diestrum (0.35 ± 0.17 ng/ml and 0.38 ± 0.15 vs 0.68 ± 0.17 ng/ml, p < 0.05). These results show that treatment with a single injection of 50 IU/kg of eCG in late anoestrous bitches successfully induced changes in follicular growth which were paralleled with changes in PRL, IGF1 and ANDR serum concentration similar to those occurring during a normally occurring oestrous cycle. In addition, our results suggest that IGF1 in the bitch could play an important role in ovarian folliculogenesis.

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“…These results are in agreement with previous findings from Stornelli et al. (), who observed that 50 UI of eCG induced follicular growth in all animals studied.…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

“…According to the percentage and type of cells present, samples were scored from 1 to 5 (Table ; Stornelli et al. ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 3 more Smart Citations

Prolactin, Androstenedione and IGF1 Serum Concentrations During Induced Follicular Growth by eCG Administration in the Bitch

Stornelli

Mitacek

Praderio

et al. 2015

Reprod Domestic Animals

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Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG

Hirata

Tanihara

Taniguchi

et al. 2018

Veterinary Medicine & Sci

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Ovarian follicular dynamics is not well known in dogs. Imaging of ovaries is technically difficult; however, ovaries clamped at a subcutaneous site can more easily be monitored using ultrasound imaging. This study investigated the follicular development of canine ovaries stimulated by hormone treatment using ultrasound imaging of the ovaries clamped at a subcutaneous site. Oestrus was induced using subcutaneous administration of 500 IU equine chorionic gonadotropin (eCG) and 1000 IU human chorionic gonadotropin (hCG) (eCG/hCG). Five bitches were given 1000 IU hCG 11 days after eCG/hCG administration. Examinations with ovarian ultrasonography using a 7.5‐MHz sector transducer, vaginal cytology, and assays of serum oestrogen and progesterone were performed daily until 20 days after eCG/hCG administration. Serosanguineous vaginal discharges and vaginal cytology of two of the bitches were observed. New follicular growth (>1.0 mm in diameter) was observed in all bitches from 2 to 8 days after eCG/hCG administration. The mean diameter of follicles and maximum numbers of follicles per ovary ranged from 2.8 to 5.5 mm and 4 to 16, respectively. The elevation in oestrogen concentrations after eCG/hCG administration was observed in all bitches, and elevation in progesterone concentration (>2 ng mL−1) was observed in three bitches. However, no follicles ovulated until 9 days after hCG administration. In conclusion, although the number of examined bitches were limited, follicular growth in ovaries clamped at a subcutaneous site can be monitored using ultrasound imaging. Ovarian ultrasonography showed that eCG/hCG administration induced new follicular growth and hCG administration induced increases in oestrogen concentrations but not ovulation by hCG administration.

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Estrous Cycle Manipulation in Dogs

Kutzler

2018

Veterinary Clinics of North America: Small Animal Practice

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Pharmacokinetics of eCG and induction of fertile estrus in bitches using eCG followed by hCG

Cited by 10 publications

References 34 publications

Prolactin, Androstenedione and IGF1 Serum Concentrations During Induced Follicular Growth by eCG Administration in the Bitch

Prolactin, Androstenedione and IGF1 Serum Concentrations During Induced Follicular Growth by eCG Administration in the Bitch

Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG

Estrous Cycle Manipulation in Dogs

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