Pharmacokinetic study of dendrobine in rat plasma by ultra‐performance liquid chromatography tandem mass spectrometry

Abstract

Dendrobine, considered as the major active alkaloid compound, has been used for the quality control and discrimination of Dendrobium which is documented in the Chinese Pharmacopoeia. In this work, a sensitive and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for determination of dendrobine in rat plasma is developed. After addition of caulophyline as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 (2.1 ×100 mm, 1.7 µm) column with acetonitrile and 0.1% formic acid as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 264.2 → 70.0 for dendrobine and m/z 205.1 → 58.0 for IS. Calibration plots were linear throughout the range 2-1000 ng/mL for dendrobine in rat plasma. The RSDs of intra-day and inter-day precision were both <13%. The accuracy of the method was between 95.4 and 103.9%. The method was successfully applied to pharmacokinetic study of dendrobine after intravenous administration. Copyright © 2015 John Wiley & Sons, Ltd.

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

“…A variety of sample preparation techniques have been applied in a bioanalytical method such as protein precipitation, liquid-liquid extraction, and solid-phase extraction [32]. Among these, the most widely used are protein precipitation and liquid-liquid extraction followed by solid-phase extraction which are used in different category such as antibiotic [33][34][35][36][37][38][39][40], anticancer [36,[41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62], antiviral [63][64][65][66][67], antifungal [68][69][70][71][72], cardiovascular [73][74][75][76][77]…”

Bioactive Secondary Metabolites from Orchids (Orchidaceae)

“…Despite the quite unusual structure of some of these compounds, limited information is available on their bioactivities. One of the most important alkaloids in Dendrobium genus is dendrobin ( 45 ), which has been used in the Chinese Pharmacopoeia for the quality control of Dendrobium raw material . A possible use of dendrobine was evaluated for the protective effects on cortical neurons injured by oxygen‐glucose deprivation/reperfusion (OGD/RP) in vitro .…”

Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MS/MS

“…Proteins and other potential interference would affect the analysis of the mass spectrometry system [ 34 , 35 ]. Therefore, an effective and simple sample preparation was a key point for establishing the UPLC-MS/MS method of TBMS-I.…”