Pharmacokinetic profile and metabolite identification of bornyl caffeate and caffeic acid in rats by high performance liquid chromatography coupled with mass spectrometry

Shi, Bowen; Yang, Lingjian; Gao, Tian; Ma, Cuicui; Li, Qiannan; Yang, Nan; Wang, Shixiang; Xiao, Chaoni; Jia, Ping; Zheng, Xiaohui

doi:10.1039/c8ra07972b

Cited by 15 publications

(11 citation statements)

References 40 publications

(31 reference statements)

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“…Correspondingly, the peak area of caffeic acid increased significantly at 4 hr, but disappeared at 24 hr, as displayed in Figure b. This is because chlorogenic acid could be hydrolyzed to caffeic acid and quininic acid by intestinal bacteria, while caffeic acid could be further metabolized (Shi et al, ). However, the levels of p ‐hydroxyacetophenone only increased to a small extent until 48 hr of incubation, suggesting that p ‐hydroxyacetophenone was hardly metabolized in the gut.…”

Section: Resultsmentioning

confidence: 94%

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Liu

Sun

Tian

et al. 2020

Biomedical Chromatography

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Qushi Huayu Fang (QHF) is a clinic-empirical prescription for treating non-alcoholic fatty liver disease (NAFLD) in China, which is composed of five herbs. However, the bioactive constituents responsible for the efficacy of QHF remain unclear. Thus, a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was established and adopted to identify the constituents of QHF, and profile its metabolism in vivo and in vitro. Among the 66 constituents in QHF, only 14 compounds of six structural types were absorbed, and 34 metabolites were generated through eight metabolic pathways. A total of 20 metabolites were first reported, including four organic acids, one iridoid, two flavones, five naphthols, three anthraquinones, and five stilbenes. Glucuronidation and sulfation were the main metabolic pathways, and the intestinal metabolism played an important role in the biotransformation of QHF. Many compounds, especially those detected in the liver, the target organ of QHF, were reported to display the anti-NAFLD activity. This is the first study to explore the constituents of QHF and its metabolism in vivo and in vitro, thus realizing the first step to clarify the chemical basis of QHF qualitatively, and laying the foundation for further research on the anti-NAFLD mechanism of QHF. K E Y W O R D S HPLC-Q-TOF, intestinal bacteria, liver, metabolism, Qushi Huayu FangAbbreviations: HPLC-Q-TOF, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry; IRE1α, inositol-requiring transmembrane kinase/endoribonuclease 1α; NAFLD, non-alcoholic fatty liver disease; Nrf-2, nuclear erythroid 2-related factor 2; PI3K, phosphatidylinositol 3-kinase; PPARα, peroxisomal proliferator-activated receptor α; QHF, Qushi Huayu Fang; SD, Sprague Dawley; SREBP-1c, sterol regulatory element-binding protein-1c; TCM, traditional Chinese medicines; TNF-α, tumor necrosis factor-α; XBP1s, X-box binding protein 1; YCHD, Yinchenhao Decoction. # Huan Liu and Zhaolin Sun contributed equally to this study.

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Section: Resultsmentioning

confidence: 94%

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Liu

Sun

Tian

et al. 2020

Biomedical Chromatography

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“…No clear difference was found between the two groups of data, which confirmed the equal dosage of mogroside V during the administration LHG and the compound alone. The ratio of the AUC value was below 120%, providing a proof of rationale time points for blood collection to pursue the pharmacokinetic investigation (Shi et al, 2019). Taking the identical pharmacokinetic behavior into account, we inferred that mogroside V is the representative compounds of LHG for treatment of β 2 -AR-mediating ailments.…”

Section: Resultsmentioning

confidence: 99%

Screening Bioactive Compounds of Siraitia grosvenorii by Immobilized β2-Adrenergic Receptor Chromatography and Druggability Evaluation

et al. 2019

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As the first and key step of traditional Chinese medicine (TCM)-guided drug development, lead discovery necessitates continuous exploration of new methodology for screening bioactive compounds from TCM. This work intends to establish a strategy for rapidly recognizing β 2-adrenergic receptor (β 2-AR) target compounds from the fruit of Siraitia grosvenorii (LHG). The method involved immobilization of β 2-AR onto amino-microsphere to synthesize the receptor column, the combination of the column to high-performance liquid chromatography (HPLC) to screen bioactive compounds of LHG, the identification of the compounds by HPLC coupled with mass spectrometry (MS), and the evaluation of druggability through pharmacokinetic examination by HPLC-MS/MS. Mogroside V was screened and identified as the β 2-AR-targeted bioactive compounds in LHG. This compound exhibited desired pharmacokinetic behavior including the time to reach peak plasma concentrations of 45 min, the relatively low elimination of 138.5 min, and the high bioavailability. These parameters indicated that mogroside V has a good druggability for the development of new drugs fighting β 2-AR-mediated respiratory ailments like asthma. The combination of the methods in this work is probably becoming a powerful strategy for screening and early evaluating the bioactive compounds specifically binding to G-proteincoupled receptor target from complex matrices including TCM.

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“…As there is a risk of overdose and/or of side effects, research has focused on new, accurate methods for determining CA. Up to the present, various analytical methods have been developed for CA determination which include liquid chromatography, capillary electrophoresis, as well as electrochemical methods [7,[66][67][68][69]. Among the methods mentioned, the electrochemical ones are preferred due to their excellent sensitivity, fast response, good selectivity, low cost, reliability, and simplicity [1,69].…”

Section: Caffeic Acid and Its Role In The Human Bodymentioning

confidence: 99%

“…electrochemical methods [7,[66][67][68][69]. Among the methods mentioned, the electrochemical ones are preferred due to their excellent sensitivity, fast response, good selectivity, low cost, reliability, and simplicity [1,69].…”

Section: Sensors Based On Carbon Nanotubesmentioning

confidence: 99%

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

Bounegru

Apetrei

2020

IJMS

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Caffeic acid is one of the most important hydroxycinnamic acids found in various foods and plant products. It has multiple beneficial effects in the human body such as antioxidant, antibacterial, anti-inflammatory, and antineoplastic. Since overdoses of caffeic acid may have negative effects, the quality and quantity of this acid in foods, pharmaceuticals, food supplements, etc., needs to be accurately determined. The present paper analyzes the most representative scientific papers published mostly in the last 10 years which describe the development and characterization of voltamperometric sensors or biosensors based on carbon nanomaterials and/or enzyme commonly used for detecting caffeic acid and a series of methods which may improve the performance characteristics of such sensors.

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Pharmacokinetic profile and metabolite identification of bornyl caffeate and caffeic acid in rats by high performance liquid chromatography coupled with mass spectrometry

Abstract: We revealed the metabolic profile of bornyl caffeate by HPLC-Q-TOF/MS, and then simultaneously examined the pharmacokinetics of bornyl caffeate and CA after administration of a single dose of bornyl caffeate by HPLC ion trap MS.

Cited by 15 publications

References 40 publications

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Screening Bioactive Compounds of Siraitia grosvenorii by Immobilized β2-Adrenergic Receptor Chromatography and Druggability Evaluation

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

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