Pharmacognostical study of Desmodium caudatum

Li, Junni; Lin, Xiaojing; Tang, Gengqiu; Li, Rong; Wang, Dong; Sheng-guo, JI

doi:10.1590/0001-3765201920180637

Cited by 9 publications

(4 citation statements)

References 22 publications

(9 reference statements)

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“…Identi cation of the varieties and assurance of the quality of herbal medicines are therefore of the utmost importance [25]. and the authentication of raw material is the rst step to assure purity in this route.…”

Section: Discussionmentioning

confidence: 99%

Pharmacognostical assessment, antioxidant ability and determination of phytomolecules using GC-MS in Gloriosa superba Linn

Singh

Rai

Kumar

et al. 2023

Preprint

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Gloriosa superba Linn (family Colchicaceae) is a tendril climber with cylindrical and V or L shaped rhizomes that has received much attention due to its ancient pharmacological uses and being a rich source of colchicine. The pharmacognostical profile of leaf, stem and rhizome were analysed to assure the grade of raw material in the proposed investigation. Moreover, the antioxidant capacity of ethanolic rhizome extract of G. superba (EREG) was assessed using DPPH radical scavenging assay. Simultaneously, we have evaluated the phytochemical profile of EREG by GC-MS and total phenolic content was performed as well. Transverse section of rhizome revealed thin layer of brownish scales that fabricate the outer skin. In addition, the cortical area made up of homogeneous fleshy and parenchymal cells filled with starch granules which is one of the superabundant diagnostic features of this plant. Pharmacognostical profile of leaf, stem and rhizome of the plant demonstrated the evidence of authenticity of this plant. Anti-scavenging capacity (IC50) and total phenolic content was 77.20% and 9.54 mg, GAE/g, respectively. The GC-MS library identified two phytoconstituents as diethyl phthalate (100% peak area) and arsenous acid, tris(trimethylsilyl) ester (22.68% peak area) whose vast benefits are reported in the fields of pharmaceuticals, industry and personal care products. Frivolous research work in the field of pharmacognosy, antioxidant ability and instrumental analysis for phytomolecules provides a vast avenue for research on G. superba.

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Section: Discussionmentioning

confidence: 99%

Pharmacognostical assessment, antioxidant ability and determination of phytomolecules using GC-MS in Gloriosa superba Linn

Singh

Rai

Kumar

et al. 2023

Preprint

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“…A small amount of the FRL powder was placed on a white porcelain reaction plate. Specific reagents were added dropwise to observe the color reaction of fluorescence presented under a UV lamp in both wavelengths (254 and 365 nm), as well as in visible light (Junni, Lin, Tang, Rong, & Shengguo, 2019).…”

Section: Methodsmentioning

confidence: 99%

Microscopic investigations and pharmacognostic techniques for the standardization of the fruits of Rosa laxa Retz

Wang

Sun

Zhang

et al. 2021

Microscopy Res & Technique

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Rosa laxa Retz., a shrub belonging to the family Rosaceae, is widely distributed in the northern foothills of the Tianshan Mountains in Xinjiang, China. The fruits of R. laxa (FRL) has antibacterial, hypolipidemic, and antioxidant effects. In this study, FRL was subjected to pharmacognostic identification of its source, morphology, microscopic characteristics, and physicochemical properties. The microscope showed that the cross-sectional features of FRL were obvious, and the FRL powder contained vessel, parenchyma cells, exocarp cells, pollen grains, and cluster crystals. Scanning electron microscopy (SEM) analysis results show that numerous villi and many small particles (particle size of 5-50 μm) were observed in the FRL powder, and there are many gullies on the surface of the particles. In addition, the secondary metabolites of FRL were characterized via ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and thin-layer chromatography (TLC). Results showed that FRL contains various secondary metabolites, including flavonoids, phenolic acids, glycosides, and tannins. Water as the extraction solvent had the highest extraction rate and the contented of total flavonoids was 2.88 mg/g, and the contented of total polyphenols was 54.89 mg/g. Moreover, TLC identification revealed that it contains catechin and tiliroside. These parameters of FRL, which are reported herein, are important to the development of the pharmacognostic standards, as well as in the identification and quality control of FRL.

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“…In the leaf epidermis analysis, two to three fresh leaves were taken and torn directly using tweezers (Li et al 2019). The steps were as follows (Zahara (2019).…”

Section: Micromorphological Characteristicsmentioning

confidence: 99%

Morphological and molecular characterization of Distichochlamys citrea M.F. Newman in Bach Ma National Park, Thua Thien Hue Province, Vietnam

et al. 2022

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Abstract. Chen TV, Tuan ND, Triet NT, An NH, Nguyen PTT, Hai NTT, Nhi NTT, Co NQ, Nhi HTH, Huong HV, Phuong TTB, Nhung NTA. 2022. Morphological and molecular characterization of Distichochlamys citrea M.F. Newman in Bach Ma National Park, Thua Thien Hue Province, Vietnam. Biodiversitas 23: 2066-2079. Distichochlamys citrea (Black Ginger or gung den) is a medicinal plant endemic to Vietnam. However, this species is not easily identified due to the lack of a detailed description. Therefore, this study aimed to characterize morphological and molecular aspects of D. citrea from Bach Ma National Park, Vietnam. Six representative plants were selected for the following analyses. Macromorphological features were observed and compared with previous studies. The rhizomes, roots, petioles, and leaves were then histologically analyzed using iodine green-carmine staining. The ground rhizomes and leaves were also microscopically examined for powder characteristics. Finally, the D. citrea DNA barcode was amplified by Internal Transcribe Spacer (ITS) primers. Macromorphologically, D. citrea differs from other Distichochlamys species. Black Ginger, particularly, has elongated rhizomes (with scars from the shoots of previous years), green leaves, spread inflorescences, and yellow labellum (with deep slits). Additionally, D. citrea’s micromorphological structures (epidermis, exodermis, hypodermis, cortex, endodermis, and root pith) are similar to the genus Zingiber. However, the absence of calcium oxalate and silica crystals in the root is unique and can be used to distinguish this plant from other Zingiberaceae members. The sequenced amplicons (96.54% similar to Genbank's D. citrea ITS) demonstrated the ITS marker’s ability to identify Black Ginger.

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Pharmacognostical study of Desmodium caudatum

Cited by 9 publications

References 22 publications

Pharmacognostical assessment, antioxidant ability and determination of phytomolecules using GC-MS in Gloriosa superba Linn

Pharmacognostical assessment, antioxidant ability and determination of phytomolecules using GC-MS in Gloriosa superba Linn

Microscopic investigations and pharmacognostic techniques for the standardization of the fruits of Rosa laxa Retz

Morphological and molecular characterization of Distichochlamys citrea M.F. Newman in Bach Ma National Park, Thua Thien Hue Province, Vietnam

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