Pharmacognostic evaluation, and development and validation of a HPLC-DAD technique for gallocatechin and epigallocatechin in rhizomes from Limonium brasiliense

Blainski, Andressa; Antonelli-Ushirobira, Tânia Mara; Godoy, Guilherme; Leite-Mello, Eneri Vieira de Souza; Mello, João Carlos Palazzo de

doi:10.1016/j.bjp.2016.08.009

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…As in the case of antioxidant activity, most EAF samples showed higher polyphenol content compared to their respective CEs. Indeed, many studies have shown a significant correlation between antioxidant capacity and TPC [19,21,36,37], reinforcing our results.…”

Section: Resultssupporting

confidence: 90%

“…Through assessment of the scatter plots ( Scores and Loadings , S1 Fig), it was possible to distinguish which plant extracts were most strongly associated with phenolic compounds, antioxidant activity and AChE inhibitory activity. The samples obtained as EAFs showed the highest content of phenolic compounds and better antioxidant and AChE inhibitory activity, as expected and already discussed [21,36,37]. The EAFs also showed better AChE inhibitory activity, probably because the relative abundance of the active constituents in these fractions was higher [34].…”

Section: Resultssupporting

confidence: 78%

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In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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Alzheimer’s disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia , Limonium brasiliense , Paullinia cupana , Poincianella pluviosa , Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ 25–35 toxicity in SH-SY5Y cells. The effect of the EAF of S . adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 ( S . adstringens , P . pluviosa and L . brasiliense ) protected SH-SY5Y cells from Aβ 25-35 -induced toxicity. The EAF of S . adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ 25-35 -induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S . adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ 25–35 , which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G . ulmifolia , L . brasiliense , P . cupana , P . pluviosa and S . adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 78%

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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“…Blainski and colleagues when validating a method by HPLC-DAD for quantification of gallocatechin and epigallocatechin in Limonium brasiliense rhizomes obtained r]esults of analysis of variance for linearity compatible and consistent with the present work [41].…”

Section: Determination Of Linearitysupporting

confidence: 71%

Development and Validation of High-Performance Liquid Chromatography With Diode Array Detector Method to Analysis of Kaempferol Marker From Extracts of Poincianella Pyramidalis (Tul) L.P Queiroz

Oliveira

Souza

et al. 2017

Asian J Pharm Clin Res

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Objective: This study aims to develop the extraction of the marker kaempferol in the fluid extract (FE) and validate an analytical method that monitors the quality of extracts of P. pyramidalis. Methods:The P. pyramidalis leaves were collected and then were dried to milling process. The extracts were drawn up at 20% weight: Volume (w/v) by maceration, and the extraction system used was hydroethanol solution ratio at 50:50 volume: Volume (v: v). From the hydroalcoholic extract, a method of extracting the kaempferol biomarker was developed and validated by high-performance liquid chromatography coupled with diode array detector. To validate a method, the following parameters were evaluated: Specificity, selectivity, linearity, limit of quantification (LOQ) and detection (LOD), precision, accuracy, robustness, and stability. Results:The method developed proved to be efficient, as it allowed the analysis of the interferents free marker, with recovery above 90%, linear over the range 1.4-26.6 µg/mL, correlation coefficient R 2 =0.999, and LOD and LOQ 0.07 and 0.22 µg/mL, respectively, specificity, precision, accuracy, and robustness. Conclusion:The extraction methodology of the kaempferol marker was successfully developed interferents free and the validated method by HPLC-DAD represents a useful tool in the quality control of P. pyramidalis herbal medicines.

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“…Therefore, there is an urgent need to ascertain the identity and consistency of plant crude drugs and their phytopharmaceutical products (Mukherjee 2002). Generally, botanical and chemical analyses are age-long quality control methods of herbal medicines (Blainski et al 2017).…”

Section: Introductionmentioning

confidence: 99%

HPLC Analysis of Gedunin in MAMA Decoction, an Antimalarial Herbal Remedy in Nigeria

Adepiti

Agbaje

Adehin

et al. 2020

Rev. Bras. Farmacogn.

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MAMA decoction is an herbal preparation certified in Nigeria for the treatment of malaria. This study evaluated some parameters with a view to provide quality control standards for the decoction. A mixture of the component leaves of Mangifera indica L., Anacardiaceae, Azadirachta indica A. Juss., Meliaceae, Morinda lucida Benth., Rubiaceae, and Alstonia boonei De Wild., Apocynaceae, was extracted using the decoction method. The filtrate was freeze-dried into a powdered extractive. Pharmacognostical and physicochemical standards, qualitative and quantitative determinations of gedunin by thin-layer chromatography and high-performance liquid chromatography were determined. The extractive yield of the decoction was about 19%, and the moisture content of the plant leaves (6% to 16%) was within the pharmacopoeial limit. Gedunin was present at 0.42 μg/ml of decoction (0.04% w/v). The freeze-dried preparation contained 0.22 μg/100 μg of extractive (0.2% w/w), while A. indica leaf had 0.011 μg gedunin/100 μg of dried leaf (0.01% w/w). The study provided a set of reproducible analytical conditions for the quality control of MAMA decoction.

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Pharmacognostic evaluation, and development and validation of a HPLC-DAD technique for gallocatechin and epigallocatechin in rhizomes from Limonium brasiliense

Cited by 12 publications

References 22 publications

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Development and Validation of High-Performance Liquid Chromatography With Diode Array Detector Method to Analysis of Kaempferol Marker From Extracts of Poincianella Pyramidalis (Tul) L.P Queiroz

HPLC Analysis of Gedunin in MAMA Decoction, an Antimalarial Herbal Remedy in Nigeria

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